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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (2): 158-164.doi: 10.3736/jcim20110208

• Original Experimental Research • Previous Articles     Next Articles

Analysis of plasma metabonomics of mini-swines with qi deficiency and blood stasis syndrome due to chronic myocardial ischemia

Yong Wang1, Shu-zhen Guo1, Chun Li1, Jian-xin Chen1, Wen-jing Chuo1, Fang Dong2, Wei Wang 1(), Ying-feng Wang   

  1. 1. School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
    2. Center for Analytical Chemistry, Capital Normal University, Beijing 100037, China
  • Received:2010-10-17 Accepted:2010-11-08 Online:2011-02-20 Published:2011-02-15
  • Contact: Wang Wei E-mail:wangwei26960@126.com

Objective: To study the changes of plasma metabolites of mini-swines with qi deficiency and blood stasis syndrome due to chronic myocardial ischemia and to explore the relationship between plasma metabonomics and qi deficiency and blood stasis syndrome.
Methods: Twenty-two mini-swines were used in this study and were divided into sham-operation group (n=10) and model group (n=12). Ameroid constrictors were placed around the left anterior descending coronary artery of mini-swines in model group to induce chronic myocardial ischemia. Twelve weeks after Ameroid placement, physical signs, coronary angiography and echocardiography were used to evaluate qi deficiency and blood stasis syndrome; gas chromatography-mass spectrometry (GC-MS) and data mining method were used to analyze plasma metabolites of the mini-swines.
Results: Twelve weeks after operation, mini-swines in model group were confirmed with qi deficiency and blood stasis syndrome. Principal component analysis (PCA) found that plasma GC-MS spectra of the two groups were significantly different. Compared with sham-operation group, endogenous metabolites in plasma were changed in model group. The levels of lipid metabolites such as glycerol, acetic acid and tricosadiynoic acid, myo-inositol, as well as amino acid metabolite proline were raised, while concentrations of glucose (eg. d-glucose, and à-D-glucoside), amino acid (eg. alanine, phenylalanine, and urea) and lipid metabolites (eg. hexadecanoic acid, propionic acid, butanoic acid, and octadecanoic acid) were declined.
Conclusion: Significant differences in metabolic spectrum exist in mini-swines with qi deficiency and blood stasis syndrome due to chronic myocardial ischemia and normal mini-swines. Disorders in glucose, amino acid and lipid metabolism result in a deterioration of coronary artery disease; citric acid, glucose, octadecanoic acid, hexadecanoic acid, glycerol and acetic acid are contributory to separation of the healthy from qi deficiency and blood stasis syndrome. These metabolites pattern may be used in clinical diagnosis and treatment of coronary artery disease, and also can provide a new approach to objective research in traditional Chinese medicine syndrome.

Key words: coronary disease, qi deficiency, blood stasis, gas chromatography-mass spectrometry, principal component analysis, metabonomics, plasma, swine, miniature

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Figure 1

Coronary angiogram of sham-operation group and model group 12 weeks after operation A: Sham-operation group; B: Model group. Arrow points to the left anterior descending coronary artery in sham-operation or model swine."

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Figure 2

GC-MS spectra of sham-operation group and model group 12 weeks after operation A: Sham-operation group; B: Model group."

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Figure 3

Principal component score plots and loading plots from analysis of GC-MS spectra of sham-operation group and model group 12 weeks after operation"

[1] He J, Gu D, Wu X, Reynolds K, Duan X, Yao C, Wang J, Chen CS, Chen J, Wildman RP, Klag MJ, Whelton PK . Major causes of death among men and women in China[J]. N Engl J Med, 2005,353(11):1124-1134
doi: 10.1056/NEJMsa050467
[2] Qi QG, Wang W, Hu LS, Bi LF, Guo SZ . Documents statistical analysis on TCM syndrome differentiation types of coronary angina pectoris: a report of 9 790 cases[J]. Shanxi Zhong Yi, 2008,24(10):31-33
doi: 10.3969/j.issn.1000-7156.2008.10.025
其其格, 王伟, 胡立胜, 毕力夫, 郭淑贞 . 9 790例冠心病心绞痛辨证分型文献统计分析[J]. 山西中医, 2008,24(10):31-33
doi: 10.3969/j.issn.1000-7156.2008.10.025
[3] Pan M, Chen Q . On body constitution, disorders and metabonomics in Chinese medicine[J]. Shanghai Zhong Yi Yao Za Zhi, 2008,42(6):53-56
doi: 10.3969/j.issn.1007-1334.2008.06.023
潘明, 陈琼 . 中医体质、病证研究与代谢组学[J]. 上海中医药杂志, 2008,42(6):53-56
doi: 10.3969/j.issn.1007-1334.2008.06.023
[4] Nicholson JK, Wilson ID . Opinion: understanding ‘global’ systems biology: metabonomics and the continuum of metabolism[J]. Nat Rev Drug Discov, 2003,2(8):668-676
doi: 10.1038/nrd1157 pmid: 12904817
[5] Shulaev V . Metabolomics technology and bioinformatics[J]. Brief Bioinform, 2006,7(2):128-139
doi: 10.1093/bib/bbl012
[6] Schwartz RS, Holmes DR . Pigs, dogs, baboons and man: lessons for stenting from animal studies[J]. J Interv Cardiol, 1994,7(4):355-368
doi: 10.1111/j.1540-8183.1994.tb00469.x
[7] Xu WY, Wang W, Guo SZ, Liu T, Liu L, Yu YX . Duplication of an animal model of myocardial ischemia with blood stasis syndrome in mini-swines[J]. J Chin Integr Med, 2008,6(4):409-411
许文玉, 王伟, 郭淑贞, 刘涛, 刘蕾, 于永新 . 小型猪心肌缺血血瘀证动物模型的复制方法[J]. 中西医结合学报, 2008,6(4):409-411
[8] Wang J, Chen KJ, Weng WL, Qian ZH, Wang Y, Liu JG, Ge ZY . Research on diagnostic criteria of blood-stasis symptom-complex[J]. Zhong Xi Yi Jie He Za Zhi, 1988,8(10):585-589
王阶, 陈可冀, 翁维良, 钱振淮, 王怡, 刘剑刚, 葛争艳 . 血瘀证诊断标准的研究[J]. 中西医结合杂志, 1988,8(10):585-589
[9] Shen ZY, Wang WJ . Reference criteria for differentiation of deficiency syndromes in traditional Chinese medicine[J]. Zhong Xi Yi Jie He Za Zhi, 1986,6(10):598
沈自尹, 王文健 . 中医虚证辨证参考标准[J]. 中西医结合杂志, 1986,6(10):598
[10] Chen JX, Zhao HH, Wang W . Investigative strategy for research on biological basis of traditional Chinese medicine syndrome: feature selection-based data mining methods[J]. J Chin Integr Med, 2010,8(8):747-749
doi: 10.3736/jcim20100803
陈建新, 赵慧辉, 王伟 . 中医证候生物学基础研究思路初探——基于特征选择的数据挖掘方法[J]. 中西医结合学报, 2010,8(8):747-749
doi: 10.3736/jcim20100803
[11] Guo SZ, Chen JX, Zhao HH, Wang W, Yi JQ, Liu L, Qi QJ, Liu RQ, Qiu Q, Wang Y . Building and evaluating an animal model for syndrome in traditional Chinese medicine in the context of unstable angina(myocardial ischemia) by supervised data mining approaches[J]. J Biol Syst, 2009,17(4):531-546
doi: 10.1142/S0218339009003022
[12] Luo HG, Ding J, Yue GX, Chen JX . Metabonomic study of syndrome of liver qi stagnation and spleen deficiency in rats[J]. J Chin Integr Med, 2007,5(3):307-313
罗和古, 丁杰, 岳广欣, 陈家旭 . 大鼠肝郁脾虚证的代谢组学研究[J]. 中西医结合学报, 2007,5(3):307-313
[13] Nie J, Dai AG, Hu RC . Regulation of hypoxia-inducible factor proline hydroxylase activity and hypoxic/ischemic diseases[J]. Guo Ji Bing Li Ke Xue Yu Lin Chuang Za Zhi, 2006,27(1):53-56
doi: 10.3969/j.issn.1673-2588.2007.01.013
聂金, 戴爱国, 胡瑞成 . 缺氧诱导因子脯氨酸羟化酶的调控与缺血缺氧性疾病[J]. 国际病理科学与临床杂志, 2006,27(1):53-56
doi: 10.3969/j.issn.1673-2588.2007.01.013
[14] Sabatine MS, Liu E, Morrow DA, Heller E , McCarroll R, Wiegand R, Berriz GF, Roth FP, Gerszten RE.Metabolomic identification of novel biomarkers of myocardial ischemia[J]. Circulation, 2005,112(25):3868-3875
doi: 10.1161/CIRCULATIONAHA.105.569137 pmid: 16344383
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