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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (6): 440-444.doi: 10.3736/jcim20040611

• Original Experimental Research • Previous Articles     Next Articles

Effect of madecassoside on depression behavior of mice and activities of MAO in different brain regions of rats

Mu-rong Liu1, Ting Han2, Yao Chen2, Lu-ping Qin2, Han-chen Zheng2, Yao-cheng Rui2   

  1. 1. Hangzhou Biomedical Hi-Tech Incubator Co., Ltd., Hangzhou, Zhejiang Province 310018, China
    2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received:2004-06-20 Online:2004-11-20 Published:2018-10-20

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Objective: To evaluate the effects of madecassoside (MC) on the depression behavior of mice and the activities of monoamine oxidase (MAO) in different rat brain regions.Methods: Imipramine as the positive contrast medicine, effects of MC on the depression behavior of mice were observed by forced swimming test and reserpine antagonist test. Moclobemide and pargyline as the positive controlled medicines, the activities of monoamine oxidase-A (MAO-A) and monoamine oxidase-B (MAO-B) in different rat brain regions were determined after intragastric administration of MC in 3 different dosages for 3 days or 21 days.Results: (1) The low, middle and high dosages of MC (i.g.) significantly reduced the immobility time of mice in forced swimming test (P<0.05). (2) MC in dosages of 10 mg/kg and 20 mg/kg prevented the lowering of temperature induced by reserpine (P<0.05), while 40 mg/kg had no significant effects on it (P>0.05). (3) With acute administration (3 days), the low, middle and high dosagey of MC (i.g.) significantly inhibited the activity of MAO-A in hippocampus (P<0.01), and the high dosage significantly inhibited the activity of MAO-A in hypothalamus (P<0.01), while the 3 dosages had no significant effects on the activity of MAO-A in cortex (P>0.05). With chronic administration (21 days), MC in 3 dosages had no significant effects on the activities of MAO-A in cortex and hypothalamus (P>0.05), and the high dosage (40 mg/kg) significantly enhanced the activity of MAO-A in hippocampus (P<0.01). (4) With acute administration, MC in dosages of 10 mg/kg and 20 mg/kg significantly inhibited the activity of MAO-B in cortex (P<0.05), and MC in dosage of 10 mg/kg significantly inhibited the activity of MAO-B in hypothalamus (P<0.05), and MC in dosage of 20 mg/kg significantly enhanced the activity of MAO-B in hippocampus (P<0.01). With chronic administration, MC of 3 dosages produced no significant effects on the activities of MAO-B in 3 different rat brain regions (P>0.05).Conclusion: These results support the idea that MC produces antidepressant effects through MAO inhibition in rat brain, which seems stronger with acute administration than chronic administration, while its mechanism remains to be further studied.

Key words: madecassoside, depression, monoamine oxidase, animals, laboratory

CLC Number: 

  • R285.5

Tab 1

Effect of MC on immobility time of mice in forced swimming test ($\bar{x}$±s )"

Group n Immobility time （s）
Untreated 10 90.7±22.0
Imipramine (20 mg/kg) 10 30.0±23.6**
Low dosage of MC (10 mg/kg) 10 47.7±24.6**
Middle dosage of MC (20 mg/kg) 10 49.0±17.2**
High dosage of MC (40 mg/kg) 10 38.3±17.0**

Tab 2

Effect of MC on temperature-lowering of mice induced by reserpine ($\bar{x}$±s )"

Group n Temperature （℃）
Normal control 10 37.73±0.15
Model control 10 37.29±0.21
Imipramine (20 mg/kg) 10 37.70±0.28*
Low dosage of MC (10 mg/kg) 10 37.60±0.10*
Middle dosage of MC (20 mg/kg) 10 37.63±0.18*
High dosage of MC (40 mg/kg) 10 37.57±0.12

Tab 3

Effect of acute MC treatment on activity of MAO-A in different rat brain regions ($\bar{x}$±s )"

Group n Activity of MAO-A (U·h-1·mg-1)
Cortex Hippocampus Hypothalamus
Untreated 8 425.4±10.3 451.7±11.5 433.7±5.8
Moclobemide (12 mg/kg) 8 426.9±6.0 427.2±8.0** 416.6±4.0**
Low dosage of MC (10 mg/kg) 8 425.6±8.7 428.9±7.3** 435.5±8.4△△
Middle dosage of MC (20 mg/kg) 8 420.9±7.7 419.2±6.4** 425.1±9.3*△
High dosage of MC (40 mg/kg) 8 419.7±4.7△ 424.9±10.1** 421.3±7.8**

Tab 4

Effect of chronic MC treatment on activity of MAO-A in different rat brain regions($\bar{x}$±s )"

Group n Activity of MAO-A (U·h-1·mg-1)
Cortex Hippocampus Hypothalamus
Untreated 8 430.9±7.1 423.8±5.9 423.2±7.5
Moclobemide (12 mg/kg) 8 429.2±6.3 433.3±5.4** 423.1±7.3
Low dosage of MC (10 mg/kg) 8 432.5±8.3 427.9±4.9 426.5±9.2
Middle dosage of MC (20 mg/kg) 8 428.3±8.8 426.3±4.7△ 425.3±7.9
High dosage of MC (40 mg/kg) 8 428.6±9.0 437.3±7.5** 423.6±7.4

Tab 5

Effect of acute MC treatment on activity of MAO-B in different rat brain regions($\bar{x}$±s )"

Group n Activity of MAO-B (U·h-1·mg-1)
Cortex Hippocampus Hypothalamus
Untreated 8 14.4±1.5 18.4±1.0 25.9±2.0
Pargyline (10 mg/kg) 8 10.7±1.2** 16.4±0.7* 21.4±1.1*
Low dosage of MC (10 mg/kg) 8 9.5±2.8* 22.0±3.3△ 21.9±2.1*
Middle doage of MC (20 mg/kg) 8 10.4±2.1* 24.6±3.0**△△ 23.4±2.5
High doage of MC (40 mg/kg) 8 17.7±2.3*△△ 19.2±2.5 27.8±3.5△

Tab 6

Effect of chronic MC treatment on activity of MAO-B in different rat brain regions ($\bar{x}$±s )"

Group n Activity of MAO-B (U·h-1·mg-1)
Cortex Hippocampus Hypothalamus
Untreated 8 13.4±2.2 17.8±2.6 19.0±3.3
Pargyline (10 mg/kg) 8 13.0±1.3 19.7±2.1 16.3±3.4
Low dosage of MC (10 mg/kg) 8 14.5±2.0 18.2±3.2 23.5±4.0△
Middle dosage of MC (20 mg/kg) 8 13.9±1.6 17.8±2.4 23.5±2.5△
High dosage of MC (40 mg/kg) 8 13.3±1.8 16.7±3.4 22.0±1.8△
[1] 秦路平, 郑汉臣, 丁如贤 , 等. 中药积雪草原植物的本草考证[J]. 中国野生植物资源, 1997,16(1):19-21
[2] 张中启, 袁莉, 罗质璞 . 积雪草提取物抑制小鼠体外脑单胺氧化酶A的活性[J]. 军事医学科学院院刊, 2000,24(2):158
doi: 10.3969/j.issn.1674-9960.2000.02.026
[3] 陈瑶, 秦路平, 芮耀诚 , 等. 积雪草提取物抗抑郁作用实验研究[J]. 中国药理学会通讯, 2002,19(1):70
[4] 张均田 . 现代药理实验方法学(第1版)[M]. 北京: 北京医科大学中国协和医科大学联合出版社, 1998,( 第1版):1062
[5] 赵维中, 章家胜, 明亮 , 等. 国产吗氯贝胺的抗抑郁作用[J]. 中国药理学通报, 1995,11(5):395-397
[6] 吕宝璋, 卢建, 安明榜 .受体学 ( 第1版)[M]. 合肥: 安徽科学技术出版社, 2000,( 第1版):355
[7] Robinson DS . Monoamine oxidase inhibitors: a new generation[J]. Psychopharmacol Bull, 2002,36(3):124-138
[8] Du L, Faludi G, Palkovits M , et al. High activity-related allele of MAO-A gene associated with depressed suicide in males[J]. Neuroreport, 2002,13(9):1195-1198
doi: 10.1097/00001756-200207020-00025
[9] Bonnet U . Moclobemide: therapeutic use and clinical studies[J]. CNS Drug Rev, 2003,9(1):97-140
doi: 10.1111/j.1527-3458.2003.tb00245.x pmid: 12595913
[10] 郑莉, 刘国卿 . 贯叶连翘提取物对单胺氧化酶与单胺递质的影响[J]. 中国药科大学学报, 2002,33(2):138-141
doi: 10.3321/j.issn:1000-5048.2002.02.016
[11] Wells DG, Bjorksten AR . Monoamine oxidase inhibitors revisited[J]. Can J Anaesth, 1989,36(1):64-74
doi: 10.1007/BF03010890
[13] Da Prada M, Kettler R, Keller HH , et al. Neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type A[J]. J Pharmacol Exp Ther, 1989,248(1):400-414
[14] Panova NG, Axenova LN, Medvedev AE . The stimulating effects of ethanol consumption on synjournal of rat brain monoamine oxidases and their sensitivity to the irreversible inhibitor, pargyline[J]. Neurosci Lett, 2000,292(1):66-68
doi: 10.1016/S0304-3940(00)01439-7
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