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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (6): 440-444.doi: 10.3736/jcim20040611

• Original Experimental Research • Previous Articles     Next Articles

Effect of madecassoside on depression behavior of mice and activities of MAO in different brain regions of rats

Mu-rong Liu1, Ting Han2, Yao Chen2, Lu-ping Qin2, Han-chen Zheng2, Yao-cheng Rui2   

  1. 1. Hangzhou Biomedical Hi-Tech Incubator Co., Ltd., Hangzhou, Zhejiang Province 310018, China
    2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received:2004-06-20 Online:2004-11-20 Published:2018-10-20

Objective: To evaluate the effects of madecassoside (MC) on the depression behavior of mice and the activities of monoamine oxidase (MAO) in different rat brain regions.Methods: Imipramine as the positive contrast medicine, effects of MC on the depression behavior of mice were observed by forced swimming test and reserpine antagonist test. Moclobemide and pargyline as the positive controlled medicines, the activities of monoamine oxidase-A (MAO-A) and monoamine oxidase-B (MAO-B) in different rat brain regions were determined after intragastric administration of MC in 3 different dosages for 3 days or 21 days.Results: (1) The low, middle and high dosages of MC (i.g.) significantly reduced the immobility time of mice in forced swimming test (P<0.05). (2) MC in dosages of 10 mg/kg and 20 mg/kg prevented the lowering of temperature induced by reserpine (P<0.05), while 40 mg/kg had no significant effects on it (P>0.05). (3) With acute administration (3 days), the low, middle and high dosagey of MC (i.g.) significantly inhibited the activity of MAO-A in hippocampus (P<0.01), and the high dosage significantly inhibited the activity of MAO-A in hypothalamus (P<0.01), while the 3 dosages had no significant effects on the activity of MAO-A in cortex (P>0.05). With chronic administration (21 days), MC in 3 dosages had no significant effects on the activities of MAO-A in cortex and hypothalamus (P>0.05), and the high dosage (40 mg/kg) significantly enhanced the activity of MAO-A in hippocampus (P<0.01). (4) With acute administration, MC in dosages of 10 mg/kg and 20 mg/kg significantly inhibited the activity of MAO-B in cortex (P<0.05), and MC in dosage of 10 mg/kg significantly inhibited the activity of MAO-B in hypothalamus (P<0.05), and MC in dosage of 20 mg/kg significantly enhanced the activity of MAO-B in hippocampus (P<0.01). With chronic administration, MC of 3 dosages produced no significant effects on the activities of MAO-B in 3 different rat brain regions (P>0.05).Conclusion: These results support the idea that MC produces antidepressant effects through MAO inhibition in rat brain, which seems stronger with acute administration than chronic administration, while its mechanism remains to be further studied.

Key words: madecassoside, depression, monoamine oxidase, animals, laboratory

CLC Number: 

  • R285.5

Tab 1

Effect of MC on immobility time of mice in forced swimming test ($\bar{x}$±s )"

Group n Immobility time (s)
Untreated 10 90.7±22.0
Imipramine (20 mg/kg) 10 30.0±23.6**
Low dosage of MC (10 mg/kg) 10 47.7±24.6**
Middle dosage of MC (20 mg/kg) 10 49.0±17.2**
High dosage of MC (40 mg/kg) 10 38.3±17.0**

Tab 2

Effect of MC on temperature-lowering of mice induced by reserpine ($\bar{x}$±s )"

Group n Temperature (℃)
Normal control 10 37.73±0.15
Model control 10 37.29±0.21
Imipramine (20 mg/kg) 10 37.70±0.28*
Low dosage of MC (10 mg/kg) 10 37.60±0.10*
Middle dosage of MC (20 mg/kg) 10 37.63±0.18*
High dosage of MC (40 mg/kg) 10 37.57±0.12

Tab 3

Effect of acute MC treatment on activity of MAO-A in different rat brain regions ($\bar{x}$±s )"

Group n Activity of MAO-A (U·h-1·mg-1)
Cortex Hippocampus Hypothalamus
Untreated 8 425.4±10.3 451.7±11.5 433.7±5.8
Moclobemide (12 mg/kg) 8 426.9±6.0 427.2±8.0** 416.6±4.0**
Low dosage of MC (10 mg/kg) 8 425.6±8.7 428.9±7.3** 435.5±8.4△△
Middle dosage of MC (20 mg/kg) 8 420.9±7.7 419.2±6.4** 425.1±9.3*
High dosage of MC (40 mg/kg) 8 419.7±4.7 424.9±10.1** 421.3±7.8**

Tab 4

Effect of chronic MC treatment on activity of MAO-A in different rat brain regions($\bar{x}$±s )"

Group n Activity of MAO-A (U·h-1·mg-1)
Cortex Hippocampus Hypothalamus
Untreated 8 430.9±7.1 423.8±5.9 423.2±7.5
Moclobemide (12 mg/kg) 8 429.2±6.3 433.3±5.4** 423.1±7.3
Low dosage of MC (10 mg/kg) 8 432.5±8.3 427.9±4.9 426.5±9.2
Middle dosage of MC (20 mg/kg) 8 428.3±8.8 426.3±4.7 425.3±7.9
High dosage of MC (40 mg/kg) 8 428.6±9.0 437.3±7.5** 423.6±7.4

Tab 5

Effect of acute MC treatment on activity of MAO-B in different rat brain regions($\bar{x}$±s )"

Group n Activity of MAO-B (U·h-1·mg-1)
Cortex Hippocampus Hypothalamus
Untreated 8 14.4±1.5 18.4±1.0 25.9±2.0
Pargyline (10 mg/kg) 8 10.7±1.2** 16.4±0.7* 21.4±1.1*
Low dosage of MC (10 mg/kg) 8 9.5±2.8* 22.0±3.3 21.9±2.1*
Middle doage of MC (20 mg/kg) 8 10.4±2.1* 24.6±3.0**△△ 23.4±2.5
High doage of MC (40 mg/kg) 8 17.7±2.3*△△ 19.2±2.5 27.8±3.5

Tab 6

Effect of chronic MC treatment on activity of MAO-B in different rat brain regions ($\bar{x}$±s )"

Group n Activity of MAO-B (U·h-1·mg-1)
Cortex Hippocampus Hypothalamus
Untreated 8 13.4±2.2 17.8±2.6 19.0±3.3
Pargyline (10 mg/kg) 8 13.0±1.3 19.7±2.1 16.3±3.4
Low dosage of MC (10 mg/kg) 8 14.5±2.0 18.2±3.2 23.5±4.0
Middle dosage of MC (20 mg/kg) 8 13.9±1.6 17.8±2.4 23.5±2.5
High dosage of MC (40 mg/kg) 8 13.3±1.8 16.7±3.4 22.0±1.8
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