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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (1): 46-49.doi: 10.3736/jcim20050114

• Original Experimental Research • Previous Articles     Next Articles

Effects of Corydalis ambailis migo total alkaloids on experimental cerebral ischemia

Xue-yong Hu1,An-sheng Sun2,Li-mei Yu2,Qin Wu2   

  1. 1. Department of Pharmacy, Nanchang Railway Central Hospital, Nanchang, Jiangxi Province 330003, China
    2. Department of Pharmacology, Zunyi Medical College, Zunyi, Guizhou Province 563003, China
  • Received:2004-04-16 Online:2005-01-20 Published:2005-01-20
  • Contact: An-sheng Sun E-mail:anshengsun@yahoo.com.cn

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Objective

To observe the protective effects of Corydalis ambailis migo total alkaloids (COAMTA) on cerebral ischemia/reperfusion injury in rats and to investigate its mechanism.

Methods

The effects of COAMTA on decapitated gasping mouse model and rat model of middle cerebral artery ischemia (2 h) / reperfusion (22 h) were observed. The neurological scale, cerebral infarcted volume and cerebral water content subjected to cerebral middle artery ischemia/reperfusion in rats were recorded. The activities of nitric oxide synthase (NOS) and superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the rats' brain were measured. Cell apoptosis in ischemic penumbral area was observed with light microscope in the method of terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL).

Results

The average gasping time of the mice (6.0 mg/kg or 9.0 mg/kg COAMTA) was significantly prolonged, the cerebral infarcted volume and cerebral water content of the rats (5.0 mg/kg or 7.5 mg/kg COAMTA) were significantly decreased, as compared with the control groups. The average activity of SOD in cerebral tissue of the rats (5.0 mg/kg or 7.5 mg/kg COAMTA) was significantly higher than that of the control groups, meanwhile, the average activity of NOS and the content of MDA declined significantly. The cell apoptosis in ischemic penumbral area of the rats (5.0 mg/kg COAMTA) was significantly inhibited as compared with the control groups.

Conclusion

COAMTA can facilitate the protection against cerebral ischemia / reperfusion damage. The mechanism is related to inhibiting the activity of NOS and lipoperoxidation, increasing the activity of SOD and decreasing the neuronal apoptosis.

Key words: Corydalis decumbens, Brain ischemia, Neurologic manifestations, Nitric oxide synthase, Superoxide dismutase, Malondialdehyde

CLC Number: 

  • R285.5

Tab 1

Effect of COAMTA on neurological scale of rats after cerebral ischemia/reperfusion ($\bar{x}$±s)"

Group n Score
Sham 14 0
NS 14 2.4±0.8
Nim （2.0 mg/kg） 14 0.5±0.5**
COAMAT （2.5 mg/kg） 14 2.1±0.8△
COAMAT （5.0 mg/kg） 14 1.8±0.7*△
COAMAT （7.5 mg/kg） 14 1.6±0.6**△

Tab 2

Effects of COAMTA on cerebral infarcted area and cerebral water content of rats after cerebral ischemia/reperfusion ($\bar{x}$±s)"

Group n Infarcted Area Water content
Sham 7 0 77.2±0.8
NS 7 41.9±3.8 81.0±1.0▲▲
Nim （2.0 mg/kg） 7 20.9±3.3** 78.0±0.9**
COAMAT （2.5 mg/kg） 7 40.6±3.0△△ 80.3±1.0△△
COAMAT （5.0 mg/kg） 7 37.8±2.9*△△ 79.9±0.9△△
COAMAT （7.5 mg/kg） 7 36.3±3.1*△△ 79.6±1.0*△

Tab 3

Effects of COAMTA on activities of NOS and SOD and content of MDA in brain of rats after cerebral ischemia/reperfusion ($\bar{x}$±s)"

Group n NOS (kU/L) SOD (nU/mg protein) MDA (nmol/mg protein)
Sham 7 1.66±0.09 50.44±4.37 1.45±0.09
NS 7 3.16±0.11▲▲ 31.87±3.38▲▲ 3.21±0.18▲▲
Nim (2.0 mg/kg) 7 1.67±0.12** 49.97±3.16** 1.58±0.51**
COAMAT (2.5 mg/kg) 7 3.06±0.14△△ 33.84±2.35△△ 3.06±0.25△△
COAMAT (5.0 mg/kg) 7 2.76±0.15**△△ 36.25±2.25*△△ 2.97±0.18*△△
COAMAT (7.5 mg/kg) 7 2.30±0.15**△△ 41.28±1.90**△△ 2.76±0.19**△
[1] 中国药科大学. 中药辞海(第2版)[M]. 北京: 中国医药科技出版社, 1996,( 第2版):2019
[2] 江西中医学院药学系. 夏天无生物碱的初步药理研究[J]. 中成药研究, 1978,1(4):13-16
[3] 王大元, 程美璋, 万春根 , 等. 夏天无生物碱对麻醉犬脑循环与下肢循环的作用[J]. 中西医结合杂志, 1986,6(8):447-479
[4] 刘忞, 冯高闳 . 夏天无总碱对大鼠血小板功能的影响[J]. 中国药理学通报, 1988,4(5):301-304
[5] Kim SR, Hwang SY, Jang YP , et al. Protopine from Corydalis ternata has anticholinesterase and antiamnesic activities[J]. Planta Med, 1999,65(3):218-221
doi: 10.1055/s-1999-13983
[6] 徐淑云, 卞如濂, 陈修 . 药理实验方法学(第2版)[M]. 北京: 人民卫生出版社, 1991,( 第2版):659
[7] Nagasawa H, Kogure K . Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion[J]. Stroke, 1989,20(8):1037-1043
doi: 10.1161/01.STR.20.8.1037
[8] Bederson JB, Pitts LH, Tsuji M , et al. Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination[J]. Stroke, 1986,17(3):472-476
doi: 10.1161/01.STR.17.3.472
[9] 张均田 . 现代药理实验方法(第1版)[M]. 北京: 北京医科大学中国协和医科大学联合出版社, 1998,( 第1版):1241
[10] 徐丽华, 顾振纶, 蒋小岗 , 等. 夏天无总碱中抗胆碱酯酶活性成分的研究[J]. 药学学报, 2002,37(11):902-903
[11] 吴淞, 沈岳良, 胡和成 , 等. 夏天无对复灌心血流动力学及心律失常的影响[J]. 中草药, 1994,25(5):259-260
[12] 刘辉, 陈俊抛 . 一氧化氮、一氧化氮合酶与脑缺血损伤[J]. 国外医学·生理病理科学与临床分册, 1999,19(2):121-123
[13] Linnik MD, Zobrist RH, Hatfield MD . Evidence supporting a role for programmed cell death in focal cerebral ischemia in rats[J]. Stroke, 1993,24(12):2002-2008
doi: 10.1161/01.STR.24.12.2002
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