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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (2): 115-118.doi: 10.3736/jcim20050210

• Original Experimental Research • Previous Articles     Next Articles

Effect of Niupo Zhibao Pellet on expression of neuronal nitric oxide synthase in brain of endotoxin-induced shock rats

Jian-hong Zhou1,Dong-feng Chen1,Shao-hui Du2,Hui Li1,Yi-wei Li1,Ru-dong Deng1,Sai-xia Zhang1   

  1. 1. Department of Anatomy, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510405, China
    2. Department of Internal Medicine, Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen, Guangdong Province 51800, China
  • Received:2004-05-27 Online:2005-03-20 Published:2005-03-20
  • Contact: Dong-feng Chen E-mail:cdf27212@21cn.com

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Objective

To investigate the effect of Niupo Zhibao Pellet (NPZBP) on the expression of neuronal nitric oxide synthase (nNOS) in the brain of endotoxin-induced shock rats.

Methods

SD rats were randomly divided into normal control group, endotoxin-induced shock model group and NPZBP-treated group. Lipopolysaccharide (LPS) (1.5 mg/kg i.v.) and D-galactosamine (D-GalN) (100 mg/kg i.p.) were administered to the rats in endotoxin-induced shock model group, as well as to the rats in NPZBP-treated group after seven-day treatment, to induce the shock. The expression of nNOS in the brain of the rats in each of the 3 groups was measured by immunohistochemical methods.

Results

In the 3 groups, nNOS immuno-positive cells distributed widely in layer Ⅱ, Ⅲ, Ⅳ of the cerebral cortex, the molecular layer of hippocampus, the polymorphic layer of the dentate gyrus, the reticular formation of brain stem, and the molecular, granular and Purkinje cell layer of the cerebellar cortex. The number of immuno-positive cells in the NPZBP-treated group was slightly higher than that of the normal control group, and signifficantly lower than that of the model group (P<0.05) in many regions of the brain, including cerebral cortex, hippocampus, brain stem and cerebellar cortex.

Conclusion

NPZBP can inhibit the over-expression of nNOS in wide area of the brain in endotoxin-induced shock rats.

Key words: Niupo Zhibao Pellet, Endotoxin-induced shock, Neuronal nitric oxide synthase, Immuno-histochemistry, Animals, Laboratory

CLC Number: 

  • R285.5

Tab 1

Numbers of nNOS immuno-positive cells in brain of rats in different groups($\bar{x}$±s, Cells)"

Group n Number of nNOS immuno-positive cells
Cerebral cortex Hippocampus Brain stem Cerebellum
Normal control 50 18.2±1.9 9.2±2.6 7.3±1.7 3.3±1.3
Model 50 23.7±3.8* 14.6±2.8* 11.4±1.5* 6.4±1.2*
NPZBP-treated 50 21.4±1.6△ 12.6±2.8△ 9.4±1.5△ 4.1±1.3△

Fig 1

nNOS immuno-positive cells in different parts of the rat brain in NPZBP-treated group (HE staining)A: Cerebral cortex (×40); B: Hippocampus (×20); C: Reticular formation of brain stem (×20); D: Cerebellar cortex (×40)"

Fig 2

nNOS immuno-positive cells in different parts of the rat brain in model group (HE staining)A: Cerebral cortex (×40); B: Hippocampus (×20); C: Reticular formation of brain stem (×20); D: Cerebellar cortex (×40)"

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