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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (2): 111-114.doi: 10.3736/jcim20050209

• Original Experimental Research • Previous Articles     Next Articles

Effects of Yiqi Huayu Recipe on gene expression pattern of normal and apoptotic chondrocytes in cervical intervertebral disc in rats

Yong-jun Wang1,Peng Sun2,Quan Zhou1,Mei Liu1,Zhi-jun Hu1,Chong-jian Zhou1,Qi Shi1   

  1. 1. Spinal Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. College of Acupuncture and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2004-09-20 Online:2005-03-20 Published:2005-03-20
  • Contact: Yong-jun Wang


To study the effects of Yiqi Huayu Recipe on the gene expression pattern in normal and apoptotic chondrocytes in the cervical intervertebral disc of rats.


The intervertebral disc endplates of rats were digested enzymatically. The apoptosis of the chondrocytes was induced by anti-Fas antibody. BiostarR-40s microarray chips were used to investigate the gene expression pattern in chondrocytes in the normal control, apoptosis and Yiqi Huayu Recipe groups. The results were scanned with Scan Array 4000 and analyzed with GenePix Pro 3.0, and then subjected to standardization and ratio analysis.


In the apoptosis group, 30 kinds of genes expressed in cervical intervertebral disc chondrocytes were screened out, in which 10 were up-regulated (ratio>2) and 20 down-regulated (ratio<0.5) as compared with the normal control group. On the other hand, in the Yiqi Huayu Recipe group, 97 kinds of genes expressed in cervical intervertebral disc chondrocytes were screened out, in which 44 were up-regulated (ratio>2) and 53 down-regulated (ratio<0.5) as compared with the apoptosis group.


There are some signal transduction pathways that control the apoptosis of the intervertebral disc chondrocytes. Yiqi Huayu Recipe regulates the gene express of the apoptotic chondrocytes in intervertebral disc. The study gives a further understanding to the mechanism of the cervical spondylosis and enriches the theories of qi and blood of traditional Chinese medicine.

Key words: Yiqi Huayu Recipe, Intervertebral disc, Chondrocytes, Apoptosis, Gene chip, Signal transduction

CLC Number: 

  • R289.5

Tab 1

mRNA preparation result"

Group mRNA result (μg) OD260/OD280
Normal control 23.04 2.09
Apoptosis 84.72 1.97
Yiqi Huayu Recipe 146.64 2.05

Fig 1

Gene chip hybridized signal of normal control and apoptosis groupsA: Fluorescent marked overlay; B: Scatter"

Fig 2

Gene chip hybridized signal of Yiqi Huayu Recipe and apoptosis groups A: Fluorescent marked overlay; B: Scatter"

[1] Gruber HE, Hanley EN Jr . Recent advances in disc cell biology[J]. Spine, 2003,28(2):186-193
doi: 10.1097/01.BRS.0000041589.59203.E3 pmid: 12544938
[2] 孙鹏, 王拥军, 施杞 . 椎间盘软骨终板细胞的形态及表型特征研究[J]. 脊柱外科杂志, 2003,1(6):346-349
doi: 10.3969/j.issn.1672-2957.2003.06.008
[3] Hashimoto S, Sctareh M, Ochs RL , et al. Fas/Fas ligand expression and induction of apoptosis in chondrocytes[J]. Arthritis Rheum, 1997,40(10):1749-1755
doi: 10.1002/(ISSN)1529-0131
[4] Gruber HE, Norton HJ, Hanley EN Jr . Anti-apoptotic effects of IGF-1 and PDGF on human intervertebral disc cells in vitro[J]. Spine, 2000,25(17):2153-2157
doi: 10.1097/00007632-200009010-00002 pmid: 10973395
[5] Lotz JC, Colliou OK, Chin JR , et al. Compression-induced degeneration of the intervertebral disc: an in vivo mouse model and finite-element study[J]. Spine, 1998,23(23):2493-2506
doi: 10.1097/00007632-199812010-00004
[6] 王拥军, 施杞, 李家顺 , 等. 大鼠退变颈椎间盘组织基因表达谱的研究[J]. 第二军医大学学报, 2002,23(12):1335-1338
[7] 王拥军, 施杞, 周重建 , 等. 益气化瘀方对大鼠颈椎间盘软骨终板内血管的影响[J]. 中国中医骨伤科杂志, 2002,10(4):1-4
doi: 10.3969/j.issn.1005-0205.2002.04.001
[8] 施杞, 周红海, 沈培芝 , 等. 益气化瘀补肾法对退变颈椎间盘II型胶原mRNA表达的影响[J]. 中国中医骨伤科杂志, 2001,9(1):1-9
doi: 10.3969/j.issn.1005-0205.2001.01.001
[9] 王拥军, 施杞, 石印玉 , 等. 芪麝颈康丸对颈椎间盘内IL-1α、IL-6、TNFα含量的影响[J]. 中国中医骨伤科杂志, 2000,8(1):23-25
[10] 周重建, 王拥军, 施杞 , 等. 益气化瘀方对大鼠腰神经根受压后神经肌肉终板再生修复的作用[J]. 中西医结合学报, 2003,1(4):277-280
[11] 王拥军, 施杞, 李家顺 , 等. 益气化瘀方调控颈椎间盘细胞基因表达谱的研究[J]. 中国中医基础医学杂志, 2003,9(4):32-37
[12] Zamorano J, Wang HY, Wang R , et al. Regulation of cell growth by IL-2: role of STAT5 in protection from apoptosis but not in cell cycle progression[J]. J Immunol, 1998,160(7):3502-3512
[13] Ariga K, Yonenobu K, Nakase T , et al. Mechanical stress-induced apoptosis of endplate chondrocytes in organ-cultured mouse intervertebral discs: an ex vivo study[J]. Spine, 2003,28(14):1528-1533
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