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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (6): 480-482.doi: 10.3736/jcim20050616

• Original Clinical Research • Previous Articles     Next Articles

Growth-inhibiting effect of psoralen plus ultraviolet-A light therapy on K562 cells

De-jie Zhang1,Shi-lin Hhuang1,Nan-nan Chen1,Yang Xiang1,Pei-man Yang2,Jin-yao Zhao2   

  1. 1. Institute of Traditional Chinese Medicine and Hematology of the People’s Liberation Army, Dalian, Liaoning Province 116021, China;
    2. Department of Histology and Embryology, Dalian Medical University, Dalian, Liaoning Province 116027, China
  • Received:2004-11-12 Online:2005-11-20 Published:2005-11-20
  • Contact: De-Jie ZHANG E-mail:zdjdl210@yahoo.com.cn

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Objective

To observe the effects of psoralen plus ultraviolet-A light (PUVA) on K562 cells and the relative mechanism.

Methods

The effects of psoralen, ultraviolet-A light and PUVA on K562 cells were assayed by monotetrazolium test (MTT). DNA content was analyzed by flow cytometry (FCM). The apoptotic rates of K562 cells treated with 40 and 80 μg/ml psoralen for 24 and 48 hours were assayed by Annexin-V-FITC/PI reagent kit on FCM respectively. The ultrastructures of apoptotic cells were observed by a transmission electron microscope (TEM).

Results

Either single psoralen therapy or single ultraviolet-A irradiation had inhibiting effect on K562 cells. The inhibiting effect of PUVA on K562 cells was stronger than that of the single psoralen therapy or single ultraviolet-A light irradiation (P<0.05). Apoptotic peak (AP) was detected by FCM. TEM test showed that K562 cells treated with PUVA were smaller, having condensed cell nucleus, assembled chromatin, disintegrated nucleus body and the majority of the cells appeared to be apoptotic conformation.

Conclusion

Psoralen has inhibiting effect on K562 cells, and the effect of PUVA is more significant. It is suggested that 10 min irradiation and 40 μg/ml terminal concentration of psoralen be probably the best choice for PUVA. The inhibiting effect of PUVA is due to apoptosis.

Key words: Psoralen, Photochemotherapy, Leukemia, Myeloid, Chronic, K562 cell line, Apoptosis

CLC Number: 

  • R733.72

Tab 1

Inhibition rates of K562 cells 48 h after PUVA ($\bar{x}$±s, %)"

Group Inhibition rates of K562 cells in culture media with different psoralen terminal concentrations
0 μg/ml 10 μg/ml 20 μg/ml 40 μg/ml 80 μg/ml
0 min 9.57±6.49 21.92±6.34 24.53±5.39 28.64±4.12 61.05±1.23
5 min 50.43±1.53* 67.10±4.59* 73.17±1.38* 78.63±1.01* 80.64±1.20*
10 min 67.28±0.75*△ 69.37±1.08*△ 77.49±0.18*△ 79.38±0.76*△ 81.11±0.31*△
15 min 65.50±1.14*△ 70.33±0.95*△ 77.26±0.53*△ 78.01±0.43*△ 79.58±0.31*△

Tab 2

Apoptotic rates of K562 cells 24 h after PUVA ($\bar{x}$±s, %)"

Group Apoptotic rates of K562 cells in culture media with different psoralen terminal concentrations
0 μg/ml 10 μg/ml 20 μg/ml 40 μg/ml 80 μg/ml
0 min 1.18±0.24 1.41±0.11 1.47±0.05 1.52±0.12 2.73±0.04
5 min 6.51±0.20* 7.99±0.34* 9.23±0.39* 11.94±0.50 17.84±0.57*
10 min 8.18±0.14*△ 10.41±0.60*△ 12.64±0.77*△ 25.58±1.50*△ 57.45±0.58*△
15 min 12.63±0.76*△▲ 15.94±0.27*△▲ 20.43±0.91*△▲ 26.61±0.39*△▲ 58.58±0.54*△▲
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