Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (6): 480-482.doi: 10.3736/jcim20050616

• Original Clinical Research • Previous Articles     Next Articles

Growth-inhibiting effect of psoralen plus ultraviolet-A light therapy on K562 cells

De-jie Zhang1,Shi-lin Hhuang1,Nan-nan Chen1,Yang Xiang1,Pei-man Yang2,Jin-yao Zhao2   

  1. 1. Institute of Traditional Chinese Medicine and Hematology of the People’s Liberation Army, Dalian, Liaoning Province 116021, China;
    2. Department of Histology and Embryology, Dalian Medical University, Dalian, Liaoning Province 116027, China
  • Received:2004-11-12 Online:2005-11-20 Published:2005-11-20
  • Contact: De-Jie ZHANG


To observe the effects of psoralen plus ultraviolet-A light (PUVA) on K562 cells and the relative mechanism.


The effects of psoralen, ultraviolet-A light and PUVA on K562 cells were assayed by monotetrazolium test (MTT). DNA content was analyzed by flow cytometry (FCM). The apoptotic rates of K562 cells treated with 40 and 80 μg/ml psoralen for 24 and 48 hours were assayed by Annexin-V-FITC/PI reagent kit on FCM respectively. The ultrastructures of apoptotic cells were observed by a transmission electron microscope (TEM).


Either single psoralen therapy or single ultraviolet-A irradiation had inhibiting effect on K562 cells. The inhibiting effect of PUVA on K562 cells was stronger than that of the single psoralen therapy or single ultraviolet-A light irradiation (P<0.05). Apoptotic peak (AP) was detected by FCM. TEM test showed that K562 cells treated with PUVA were smaller, having condensed cell nucleus, assembled chromatin, disintegrated nucleus body and the majority of the cells appeared to be apoptotic conformation.


Psoralen has inhibiting effect on K562 cells, and the effect of PUVA is more significant. It is suggested that 10 min irradiation and 40 μg/ml terminal concentration of psoralen be probably the best choice for PUVA. The inhibiting effect of PUVA is due to apoptosis.

Key words: Psoralen, Photochemotherapy, Leukemia, Myeloid, Chronic, K562 cell line, Apoptosis

CLC Number: 

  • R733.72

Tab 1

Inhibition rates of K562 cells 48 h after PUVA ($\bar{x}$±s, %)"

Group Inhibition rates of K562 cells in culture media with different psoralen terminal concentrations
0 μg/ml 10 μg/ml 20 μg/ml 40 μg/ml 80 μg/ml
0 min 9.57±6.49 21.92±6.34 24.53±5.39 28.64±4.12 61.05±1.23
5 min 50.43±1.53* 67.10±4.59* 73.17±1.38* 78.63±1.01* 80.64±1.20*
10 min 67.28±0.75* 69.37±1.08* 77.49±0.18* 79.38±0.76* 81.11±0.31*
15 min 65.50±1.14* 70.33±0.95* 77.26±0.53* 78.01±0.43* 79.58±0.31*

Tab 2

Apoptotic rates of K562 cells 24 h after PUVA ($\bar{x}$±s, %)"

Group Apoptotic rates of K562 cells in culture media with different psoralen terminal concentrations
0 μg/ml 10 μg/ml 20 μg/ml 40 μg/ml 80 μg/ml
0 min 1.18±0.24 1.41±0.11 1.47±0.05 1.52±0.12 2.73±0.04
5 min 6.51±0.20* 7.99±0.34* 9.23±0.39* 11.94±0.50 17.84±0.57*
10 min 8.18±0.14* 10.41±0.60* 12.64±0.77* 25.58±1.50* 57.45±0.58*
15 min 12.63±0.76*△▲ 15.94±0.27*△▲ 20.43±0.91*△▲ 26.61±0.39*△▲ 58.58±0.54*△▲
[1] Lebwohl M, Drake L, Menter A , et al. Consensus conference: acitretin in combination with UVB or PUVA in the treatment of psoriasis[J]. Am Acad Dermatol, 2001,45(4):544-553
doi: 10.1067/mjd.2001.116347
[2] Yoo EK, Rook AH, Elenitsas R , et al. Apoptosis induction of ultraviolet light A and photochemotherapy in cutaneous T-cell lymphoma: relevance to mechanism of therapeutic action[J]. J Invest Dermatol, 1996,107(2):235-242
doi: 10.1111/1523-1747.ep12329711
[3] 崔小庆, 杨易灿 . 补骨脂素加长波紫外线抗肿瘤的实验研究和应用[J]. 国外医学·肿瘤学分册, 1991,18(3):157-160
[4] 闫晓光, 周训银, 吴军正 . 补骨脂素类抗肿瘤作用的光敏特性研究[J]. 国外医学·肿瘤学分册, 1999,26(2):83-86
[5] 蔡宇, 曹克俭, 殷忠东 , 等. 补骨脂素胶囊对急性白血病多药耐药逆转作用的临床观察[J]. 中国中医药科技, 2002,9(1):53
[6] 李振波, 丘和明, 刘国普 , 等. 清毒饮和养正片对环磷酰胺所致的造血抑制小鼠粒系造血的影响[J]. 广州中医药大学学报, 1999,16(1):34-37
[1] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[2] Rebecca Kulbida, Alexander Mathes, Johannes Loeser. Beneficial effects of hirudotherapy in a chronic case of complex regional pain syndrome. Journal of Integrative Medicine, 2019, 17(5): 383-386.
[3] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Apirada Sucontphunt, Mattaka Khongkow, Thongchai Koobkokkruad, Nontima Vardhanabhuti. Flavonoids kaempferide and 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells. Journal of Integrative Medicine, 2019, 17(4): 288-295.
[4] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[5] Carlos R. Oliveira, Daniel G. Spindola, Daniel M. Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S. Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. Journal of Integrative Medicine, 2019, 17(2): 132-140.
[6] Bruno José Martins Da Silva, Sandro Wilson Gomes Pereira, Ana Paula Drummond Rodrigues, José Luiz Martins Do Nascimento, Edilene Oliveira Silva. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, 2018, 16(6): 404-410.
[7] Xin-fang Zhang, Shui-ying Xiang, Wen-ye Geng, Wen-juan Cong, Jing Lu, Chuan-wei Jiang, Kun Wang, Zi-bing Liu . Electro-acupuncture regulates the cholinergic anti-inflammatory pathway in a rat model of chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2018, 16(6): 418-426.
[8] Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan . Casticin from Vitex species: A short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 2018, 16(3): 147-152.
[9] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[10] Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.
[11] MesfinYimam, Young-Chul Lee, Breanna Moore, Ping Jiao, MeiHong, Jeong-Bum Nam, Mi-Ran Kim, Eu-Jin Hyun, Min Chu, Lidia Brownell, Qi Jia. Analgesic and anti-inflammatory effects of UP1304, a botanical composite containing standardized extracts of Curcuma longa and Morus alba. Journal of Integrative Medicine, 2016, 14(1): 60-68.
[12] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
[13] Arthur Yin Fan. The methodology flaws in Hinman’s acupuncture clinical trial, Part III: Sample size calculation. Journal of Integrative Medicine, 2015, 13(4): 209-211.
[14] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[15] Arthur Yin Fan. The methodology flaws in Hinman’s acupuncture clinical trial, Part II: Zelen design and effectiveness dilutions. Journal of Integrative Medicine, 2015, 13(3): 136-139.
Full text



[1] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[2] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[3] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[8] Bing Hu , Hong-mei An , Ke-ping Shen , Qin Du. Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism. Journal of Chinese Integrative Medicine, 2010, 8(11): 1048-1052
[9] Ya-bing Zhou, Jian-er Yu, Jie Wu, Li Bai, Li-li Huo, Xin-guang Zhang, Li-qing Li . Effects of Chinese herbal medicine Bushen Gubiao Recipe on toll-like receptor 4 and CD4 +CD25 +foxp3 +regulatory T cells in mice with recurrent respiratory tract infections. Journal of Chinese Integrative Medicine, 2010, 8(11): 1053-1059
[10] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074