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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (6): 476-479.doi: 10.3736/jcim20050615

• Original Clinical Research • Previous Articles     Next Articles

Experimental study on effects of Shengmai Injection: enhancing 5-FU anti-tumor efficacy and reducing its toxicity

Zhen Chen,Peng Wang,Wen-xia Huang,Lu-ming Liu   

  1. Department of Traditional Chinese Medicine, Cancer Hospital, Fudan University, Shanghai 200032, China
  • Received:2004-12-20 Online:2005-11-20 Published:2005-11-20
  • Contact: Zhen CHEN E-mail:cz@133sh.com

Objevtive

To observe the effects of Shengmai Injection on enhancing efficacy and reducing toxicity of 5-fluorouracil (5-FU).

Methods

Fifty hepatoma 22 bearing mice were randomly divided into five groups: control group, 5-Fu group, Shengmai Injection (low, medium and high dose) combined with 5-FU groups. There were 10 mice in each group. Mice in the five groups were injected introperitoneally the same amount of normal saline, 5-FU (20 mg·kg –1·d –1) and Shengmai Injection (3.5 ml·kg –1·d –1, 7 ml·kg –1·d –1 and 14 ml·kg –1·d –1) combined with 5-FU respectively, once a day for 14 days. After that, all mice were killed and the tumor inhibiting rates, index of immunological function, liver and kidney function and the blood cells in the peripheral blood were observed.

Results

The tumor inhibiting rates were higher in each Shengmai Injection combined with 5-FU group than that in the 5-FU group (P<0.05). The levels of CD3, CD4, CD4/CD8, IgG, IgM in 5-FU group were lower (P<0.05), while those in the three Shengmai Injection combined with 5-FU groups were higher than those in the control group (P<0.05). The level of serum alanine aminotransferase (ALT) was higher and the WBC and PLT counts in the peripheral blood were lower in 5-FU group than those in the control group (P<0.05). But the levels of serum ALT in the three Shengmai Injection combined with 5-FU groups were consistent with that in the control group and the amounts of WBC decreased slightly.

Conclusion

Shengmai Injection can enhance the anti-tumor effect of 5-FU. It can also improve the immunological function and reduce the adverse reactions of chemotherapy.

Key words: Tumor inhibiting rates, Immunity, Adverse reaction, 5-Fluorouracil, Shengmai Injection

CLC Number: 

  • R73-36

Tab 1

Effect of Shengmai Injection on T-lymphocyte subgroups ($\bar{x}$±s )"

Group n CD3 (%) CD4 (%) CD8 (%) CD4/CD8
Control group 10 68.21±2.02 40.53±6.62 20.63±3.47 2.00±0.40
5-FU group 10 64.70±4.00* 34.78±4.06* 21.30±3.16 1.66±0.24*
High-dose Shengmai Injection + 5-FU group 10 70.17±1.33* 46.97±6.51* 17.80±2.03* 2.68±0.49*
Medium-dose Shengmai Injection + 5-FU group 10 71.69±3.55* 46.80±4.45* 16.92±2.99* 2.85±0.61*
Low-dose Shengmai Injection + 5-FU group 10 70.98±2.44* 46.77±4.14* 16.78±2.41* 2.84±0.52*

Tab 2

Effect of Shengmai Injection on humoral immune function ($\bar{x}$±s, g/L)"

Group n IgA IgG IgM
Control group 10 0.036±0.014 1.306±0.038 0.168±0.017
5-FU group 10 0.026±0.005 1.266±0.038* 0.151±0.012*
High-dose Shengmai Injection + 5-FU group 10 0.030±0.012 1.369±0.076* 0.188±0.019*
Medium-dose Shengmai Injection + 5-FU group 10 0.030±0.009 1.380±0.077* 0.184±0.014*
Low-dose Shengmai Injection + 5-FU group 10 0.031±0.009 1.351±0.037* 0.191±0.021*

Tab 3

Protective effect of Shengmai Injection on liver and renal function ($\bar{x}$±s )"

Group n ALT (U/L) Alb (g/L) BUN (mmol/L)
Control group 10 33.8±2.7 31.6±2.3 6.1±1.3
5-FU group 10 41.6±3.5* 30.6±2.9 7.0±0.4
High-dose Shengmai Injection + 5-FU group 10 35.8±2.6 30.5±1.5 6.5±0.6
Medium-dose Shengmai Injection + 5-FU group 10 35.0±8.0 31.3±2.9 6.2±0.9
Low-dose Shengmai Injection + 5-FU group 10 36.7±4.2 31.0±2.9 6.2±0.8

Tab 4

Effect of Shengmai Injection on peripheral blood cell count ($\bar{x}$±s )"

Group n WBC (×109/L) Hb (g/L) PLT (×109/L)
Control group 10 6.4±2.0 117.8±11.0 68.7±7.0
5-FU group 10 3.6±1.3* 107.9±11.0 56.3±10.5*
High-dose Shengmai Injection+5-FU group 10 4.6±0.4 112.0±14.0 66.2±7.0
Medium-dose Shengmai Injection+5-FU group 10 4.6±0.5 109.2±14.2 66.1±7.5
Low-dose Shengmai Injection+5-FU group 10 4.8±0.6 112.4±11.3 65.9±7.7
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