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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (5): 514-517.doi: 10.3736/jcim20060515

• Original Experimental Research • Previous Articles     Next Articles

Effects of Cordyceps sinensis on dimethylnitrosamine-induced liver fibrosis in rats

Feng-hua Li1,Ping Liu1,Wei-guo Xiong1,Guang-fu Xu2   

  1. 1. Institute of Liver Diseases,Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Department of Traditonal Chinese Medicine, Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China
  • Online:2006-09-30 Published:2006-09-15

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Objective

to study the effects of cordyceps sinensis on dimethylnitrosamine-induced liver fibrosis in rats.

Methods

SD rats were divided into normal control group, untreated group and cordyceps sinensis-treated group. the rats in each group were fed with corresponding drug for 4 weeks. the rat's liver collagen deposition was observed with collagen staining. hydroxyproline (hyp) contents in liver tissue of the rats in 3 groups were determined with HCI hydrolysis. the tissue inhibitor of metalloproteinase-2(timp-2) and type Ⅳ collagen contents were observed by envision, and matrix metalloproteinases-2 (mmp-2) activity was detected by the method of enzyme-picture. type Ⅰ collagen was detected by western blotting. 

Results

the contents of hyp, timp-2, type Ⅳ collagen, and the expression of type Ⅰ collagen in untreated group were significantly higher than those in the normal control group, while those in cordyceps sinensis-treated group were significantly lower than those in the untreated group. the content of mmp-2 in untreated group was significantly lower than that in the normal control group, while that in cordyceps sinensis-treated group was significantly higher than that in the untreated group.

Conclusion

cordyceps sinensis can considerably relieve the liver fibrosis, and the mechanism may be related to promoting the degradation of the collagens.

Key words: Liver fibrosis, Dimethylnitrosamine, Corydyceps sinensis, Hypha, Rats

CLC Number: 

  • R285.5

Table 1

Liver tissue collagen deposition in rats"

Group n Semi-quantitative analysis
– + ++ +++ ++++
Normal control 7 7 0 0 0 0
Untreated 11 0 1 1 4 5
CS-treated 7 0 2 4 1 0

Figure 1

Liver tissue collagen staining (Masson staining×100)A: Normal control group; B: Untreated group; C: CS-treated group."

Table 2

Hyp content in liver tissue of rats（ヌ±S）"

Group n Hyp (mg/gTP)
Normal control 7 3.82±1.32
Untreated 11 18.82±4.84**
CS-treated 7 9.65±4.32△△

Table 3

Type Ⅳ collagen and TIMP-2 contents and MMP-2 activity in liver tissue of rats （ヌ±S ）"

Group n Type Ⅳ collagen (%) MMP-2 (A·g-1·L-1) TIMP-2 (%)
Normal control 6 5.3±0.4 3558.1±245.9 4.5±0.7
Untreated 6 8.1±0.5** 2351.2±551.1** 11.5±2.4**
CS-treated 6 6.0±0.5△ 3552.8±245.9△△ 5.9±1.2△△

Figure 2

TIMP-2 staining in liver tissue of rats (Envision×200)"

Figure 3

Expression of type Ⅰ collagen in liver tissue of rats (Western blotting)"

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