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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (8): 939-947.doi: 10.3736/jcim20120816

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Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats

Karuna A. Talpate1, Uma A. Bhosale2(), Mandar R. Zambare1   

  1. 1. Department of Pharmacology, Sinhgad College of Pharmacy, Vadgaon, Pune 411041, Maharashtra, India
    2. Department of Pharmacology, Smt. Kashibai Navale Medical College and General Hospital, Narhe, Pune 411041, Maharashtra, India
  • Received:2012-02-08 Accepted:2012-02-27 Online:2012-08-20 Published:2018-08-15
  • Contact: A. Bhosale Uma E-mail:umabhosale2000@gmail.com

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Objective: To study the antidiabetic, neurochemical-antioxidant and cognition protective effects of Clitorea ternatea leaves on a rat model of diabetic cognitive decline.

Methods: Antidiabetic activity was evaluated by serum glucose and body weight estimation in ethanol extract of Clitorea ternatea (EECT)-treated diabetic rats. Effects of EECT on spatial working memory (SWM) and spatial reference memory (SRM) were evaluated by Y-maze and Morris water maze tests respectively. Neurochemical-antioxidant effects of EECT were studied by acetylcholinesterase assay, and measurements of thiobarbituric acid reactive substances (TBARSs), superoxide dismutase (SOD) and catalase (CAT) levels in diabetic rats.

Results: : The 200 and 400 mg/kg of EECT showed a significant antidiabetic activity by decreasing serum glucose level (P<0.05, P<0.01), and there was a significant increase in the body weight in 400 mg/kg of EECT-treated diabetic rats (P<0.01). EECT was found to cause significant increases in SWM and SRM in retention trials on Y-maze and Morris water maze respectively (P<0.05, P<0.01). Significant decreases in acetylcholinesterase activity and TBARS level, and significant increase in CAT level were observed in rats treated with 200 and 400 mg/kg of EECT compared with rats in the diabetic control group (P<0.05 or P<0.01). Significant increase was also found in SOD in rats treated with 400 mg/kg of EECT.

Conclusion: Clitorea ternatea exhibits antidiabetic and antioxidant activities, offers the protection against diabetes-induced cognitive decline, and warrants the need for further studies to elucidate its mode of action.

Key words: diabetes mellitus, hypoglycemic agents, plants, medicinal, neurobehavioral manifestations, rats, Sprague-Dawley

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Group n Serum glucose level (mg/dL)
Normal control 6 94.20±6.96
Diabetic control 6 280.60±18.11**
EECT (200 mg/kg) 6 153.60±22.59△
EECT (400 mg/kg) 6 125.20±19.26△△
Metformin (200 mg/kg) 6 80.88±7.53△△
Piracetam (200 mg/kg) 6 248.50±14.59

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Group n Body weight (g)
Normal control 6 206.00±6.78
Diabetic control 6 150.00±7.07**
EECT (200 mg/kg) 6 164.00±9.27
EECT (400 mg/kg) 6 207.00±11.58△△
Metformin (200 mg/kg) 6 204.00±5.09△△
Piracetam (200 mg/kg) 6 160.00±8.94

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Group n Acetylcholinesteras
(μmol/(L·min·g) of tissue)
Normal control 6 3.67±0.47
Diabetic control 6 6.36±0.30**
EECT (200 mg/kg) 6 5.45±0.25△
EECT (400 mg/kg) 6 4.07±0.42△△
Metformin (200 mg/kg) 6 6.65±0.44
Piracetam (200 mg/kg) 6 3.15±0.20△△

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Group n TBARS (MDA nmol/mg of protein) SOD (μg/mg of protein) CAT (μg/mg of protein)
Normal control 6 23.83±0.57 108.40±0.37 4.33±0.05
Diabetic control 6 52.70±2.36** 47.85±2.98** 2.19±0.26**
EECT (200 mg/kg) 6 35.50±2.09△△ 52.35±7.48 3.87±0.65△
EECT (400 mg/kg) 6 25.14±0.27△△ 86.61±1.09△△ 3.46±0.28△△
Metformin (200 mg/kg) 6 39.88±0.38△△ 83.99±1.05△△ 3.72±0.05△△
Piracetam (200 mg/kg) 6 25.80±0.32△△ 61.37±0.50△△ 2.64±0.34

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Group n Spontaneous alternations (%)
Normal control 6 50.40±10.50
Diabetic control 6 34.27±2.52*
EECT (200 mg/kg) 6 52.50±5.83△
EECT (400 mg/kg) 6 58.98±6.77△
Metformin (200 mg/kg) 6 56.24±9.10△
Piracetam (200 mg/kg) 6 61.76±1.57△△

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Group n Transfer latency (s)
Normal control 6 54.2±4.54
Diabetic control 6 77.8±1.42**
EECT (200 mg/kg) 6 63.2±6.00△
EECT (400 mg/kg) 6 56.4±1.28△△
Metformin (200 mg/kg) 6 68.8±3.83
Piracetam (200 mg/kg) 6 52.6±2.65△△
[1] American Diabetes Association . Office guide to diagnosis and classification of diabetes mellitus and other categories of glucose intolerance[J]. Diabetes Care, 1992,15(suppl 2):4
[2] Blennow K, de Leon MJ, Zetterberg H . Alzheimer’s disease[J]. Lancet, 2006,368(9533):387-403
doi: 10.1016/S0140-6736(06)69113-7
[3] Zhao Y, Ye W, Boye KS, Holcombe JH, Hall JA, Swindle R . Prevalence of other diabetes-associated complications and comorbidities and its impact on health care charges among patients with diabetic neuropathy[J]. J Diabetes Complications, 2010,24(1):9-19
doi: 10.1016/j.jdiacomp.2008.09.001
[4] Bartus RT , Dean RL 3rd, Beer B, Lippa AS.The cholinergic hypojournal of geriatric memory dysfunction[J]. Science, 1982,217(4558):408-414
doi: 10.1126/science.7046051
[5] Artola A . Diabetes-, stress- and ageing-related changes in synaptic plasticity in hippocampus and neocortex — the same metaplastic process? Eur[J] Pharmacol. 2008; 585(1):153-162.
[6] Mares D, Rai MK. Plant-derived antimycotics: current trends and future prospects[M]. Binghamton: Food Products Press, 2003, 343-351
[7] Valiathan MS.Healing plants[J].Curr Sci , 1998, 75: 1122-1127
[8] Ha H , Lee HB.Reactive oxygen species as glucose signaling molecules in mesangial cells cultured under high glucose[J].Kidney Int Suppl, 2000, 77: S19-S25
[9] Baynes JW . Role of oxidative stress in development of complications in diabetes[J]. Diabetes, 1991,40(4):405-412
doi: 10.2337/diabetes.40.4.405 pmid: 2010041
[10] White L, Petrovitch H, Hardman J, Nelson J, Davis DG, Ross GW, Masaki K, Launer L, Markesbery WR.Cerebrovascular pathology and dementia in autopsied Honolulu-Asia Aging Study participants[J].Ann N Y Acad Sci, 2002, 977: 9-23
doi: 10.1111/j.1749-6632.2002.tb04794.x pmid: 12480729
[11] S Roriz-Filho J, Sá-Roriz TM, Rosset I, Camozzato AL, Santos AC, Chaves ML, Moriguti JC, Roriz-Cruz M .( Pre)diabetes, brain aging, and cognition[J]. Biochim Biophys Acta, 2009,1792(5):432-443
doi: 10.1016/j.bbadis.2008.12.003 pmid: 19135149
[12] Mukherjee PK, Kumar V, Mal M, Houghton PJ . Acetylcholinesterase inhibitors from plants[J]. Phytomedicine, 2007,14(4):289-300
doi: 10.1016/j.phymed.2007.02.002
[13] Jain NN, Ohal CC, Shroff SK, Bhutada RH, Somani RS, Kasture VS, Kasture SB . Clitoria ternatea and the CNS[J]. Pharmacol Biochem Behav, 2003,75(3):529-536
doi: 10.1016/S0091-3057(03)00130-8 pmid: 12895670
[14] Parimaladevi B, Boominathan R, Mandal SC . Evaluation of antipyretic potential of Clitoria ternatea L. extract in rats[J]. Phytomedicine, 2004,11(4):323-326
doi: 10.1078/0944711041495191 pmid: 15185845
[15] Kulkarni C, Pattanshetty JR, Amruthraj G . Effect of alcoholic extract of Clitoria ternatea Linn. on central nervous system in rodents[J]. Indian J Exp Biol, 1988,26(12):957-960
pmid: 3254867
[16] Nadkarni KM, Nadkarni AK . Nadkarni’s Indian materia medica: With Ayurvedic, Unani-tibbi, Siddha, allopathic, homeopathic, naturopathic and home remedies, appendicces and indexes.6th ed[M]. Mumbai: Popular Prakashan, 1976, 354-355
[17] Clitorea ternatea Linn . on changes in serum sugar level and small intestinal mucosal carbohydrase activities in alloxan diabetes[J]. Calcut Med J, 1990,87(11-12):168-171
[18] Kirtikar KR, Basu BD . Indian medicinal plants.2nd ed[M]. Allahabad: L.M. Basu, 1935, 801-802
[19] Khandelwal KR . Practical pharmacognosy.19th ed[M]. Pune: Nirali Prakashan, 2008, 149-156
[20] Organization for Economic Co-operation and Development. OECD guidelines for the testing of chemicals. No. 423. 2001.
[21] Wu KK, Huan Y. Streptozocin-induced diabetic models in mice and rats. Curr Protoc Pharmacol . 2008; Chapter 5: Unit 5. 47.
doi: 10.1002/0471141755.ph0547s40 pmid: 22294227
[22] Govindarajan R, Vijayakumar M, Rao CV, Kumar V, Rawat AKS, Pushpangadan P . Action of Asparagus racemosus against streptozotocin-induced oxidative stress[J]. Nat Prod Sci, 2004,10(4):177-181
[23] Trinder P . Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor[J]. Ann Clin Biochem, 1969,6(24):24-27
doi: 10.1177/000456326900600108
[24] Stanely Mainzen Prince P, Menon VP, Gunasekaran G . Hypolipidaemic action of Tinospora cordifolia roots in alloxan diabetic rats[J]. J Ethnopharmacol, 1999,64(1):53-57
doi: 10.1016/S0378-8741(98)00106-8 pmid: 10075122
[25] Ellman GL, Courtney KD, Andres V Jr, Featherstone RM .A new and rapid colorimetric determination of acetylcholinesterase activity[J].Biochem Pharmacol, 1961, 7: 88-95
doi: 10.1016/0006-2952(61)90145-9
[26] Wills ED . Mechanisms of lipid peroxide formation in animal tissues[J]. Biochem J, 1966,99(3):667-676
doi: 10.1042/bj0990667
[27] Kono Y . Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase[J]. Arch Biochem Biophys, 1978,186(1):189-195
doi: 10.1016/0003-9861(78)90479-4
[28] Claiborne A. Catalase activity. In: Greenwald RA.Handbook of methods for oxygen radical research[M]. Boca Raton: CRC Press, 1985, 283-284
[29] Hritcu L, Clicinschi M, Nabeshima T . Brain serotonin depletion impairs short-term memory, but not long-term memory in rats[J]. Physiol Behav, 2007,91(5):652-657
doi: 10.1016/j.physbeh.2007.03.028 pmid: 17481676
[30] Morris JB. Legume genetic resources with novel “value added” industrial and pharmaceutical use. In: Janick J.Prospectives on new crops and new uses[M]. Alexandria: ASHS Press, 1999, 196-201
[31] Tuzcu M, Baydas G . Effect of melatonin and vitamin E on diabetes-induced learning and memory impairment in rats[J]. Eur J Pharmacol, 2006,537(1-3):106-110
doi: 10.1016/j.ejphar.2006.03.024 pmid: 16626697
[32] Ott A , Stolk RP , van Harskamp F , Pols HA,Hofman A , Breteler MM.Diabetes mellitus and the risk of dementia: The Rotterdam Study[J]. Neurology, 1999,53(9):1937-1942
doi: 10.1212/WNL.53.9.1937
[33] Peila R, Rodriguez BL, Launer LJ; Honolulu-Asia Aging Study.Type 2 diabetes, APOE gene, and the risk for dementia and related pathologies: The Honolulu-Asia Aging Study[J]. Diabetes, 2002,51(4):1256-1262
doi: 10.2337/diabetes.51.4.1256
[34] Miyajima H, Takahashi Y, Kono S . Aceruloplasminemia, an inherited disorder of iron metabolism[J]. Biometals, 2003,16(1):205-213
doi: 10.1023/A:1020775101654 pmid: 12572680
[35] Maiese K, Morhan SD, Chong ZZ . Oxidative stress biology and cell injury during type 1 and type 2 diabetes mellitus[J]. Curr Neurovasc Res, 2007,4(1):63-71
doi: 10.2174/156720207779940653
[36] Ghosh D, Konishi T . Anthocyanins and anthocyanin-rich extracts: role in diabetes and eye function[J]. Asia Pac J Clin Nutr, 2007,16(2):200-208
doi: 10.6133/apjcn.2007.16.2.01 pmid: 17468073
[37] Sanders RA, Rauscher FM , Watkins JB 3rd.Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats[J]. J Biochem Mol Toxicol, 2001,15(3):143-149
doi: 10.1002/jbt.11 pmid: 11424224
[38] Patil CS, Singh VP, Kulkarni SK . Modulatory effect of sildenafil in diabetes and electroconvulsive shock-induced cognitive dysfunction in rats[J]. Pharmacol Rep, 2006,58(3):373-380
[39] Aragno M, Mastrocola R, Brignardello E, Catalano M, Robino G, Manti R, Parola M, Danni O, Boccuzzi G . Dehydroepiandrosterone modulates nuclear factor-κB activation in hippocampus of diabetic rats[J]. Endocrinology, 2002,143(9):3250-3258
doi: 10.1210/en.2002-220182
[40] Fadda F, Melis F, Stancampiano R . Increased hippocampal acetylcholine release during a working memory task[J]. Eur J Pharmacol, 1996,307(2):R1-R2
doi: 10.1016/0014-2999(96)00289-0 pmid: 8832228
[41] Ragozzino ME, Gold PE . Glucose injections into the medial septum reverse the effects of intraseptal morphine infusions on hippocampal acetylcholine output and memory[J]. Neuroscience, 1995,68(4):981-988
doi: 10.1016/0306-4522(95)00204-V
[42] Dougherty KD, Turchin PI, Walsh TJ . Septocingulate and septohippocampal cholinergic pathways: involvement in working/episodic memory[J]. Brain Res, 1998,810(1-2):59-71
doi: 10.1016/S0006-8993(98)00870-1 pmid: 9813241
[43] Akkol EK, Orhan IE, Yesilada E . Anticholinesterase and antioxidant effects of the ethanol extract, ethanol fractions and isolated flavonoids from Cistus laurifolius L. leaves[J]. Food Chemistry, 2012,131(2):626-631
doi: 10.1016/j.foodchem.2011.09.041
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