Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (8): 911-917.doi: 10.3736/jcim20120812

Previous Articles     Next Articles

Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy

Xin-min Li(), Hui Chen, Yan-yan Ren, Yan-li Lu#br#   

  1. Department of Paediatrics, the First Affiliated Hospital, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • Received:2011-11-09 Accepted:2011-11-21 Online:2012-08-20 Published:2018-08-15
  • Contact: Xin-min Li

Objective: To study the effects of Xifeng Capsule, a compound traditional Chinese herbal medicine, combined with carbamazepine on spontaneous epileptic seizure induced by lithium and pilocarpine in rats and the expression level of multidrug resistance-associated protein 1 (MRP1).

Methods: Lithium and pilocarpine were used to induce epilepsies in rats. All epileptic rats were randomly divided into model, high-dose Xifeng Capsule, medium-dose Xifeng Capsule, low-dose Xifeng Capsule, high-dose Xifeng Capsule plus carbamazepine (CBZ) (combined high-dose group), high-dose Xifeng Capsule plus half dose of CBZ (combined low-dose group) and CBZ groups with 10 rats in each group. And another 10 normal rats served as control. After treating 28 d, immunohistochemical method was used to detect the MRP1 expression in cortex and hippocampus of the epileptic rats.

Results: : MRP1 expression in hippocampus of the treated groups was higher than that of the normal control group, with wider range and darker positive particles, but was lower than that of the model group. In the cortical areas, the differences between the combined high-dose group or the combined low-dose group and the model group were statistically significant (P<0.05). Regardless of the hippocampus CA1, CA3, gyrus or cortical areas, the influence of high-dose Xifeng Capsule on MRP1 distribution was superior to that of low-dose Xifeng Capsule; Xifeng Capsule combined with CBZ had better effects than low-dose Xifeng Capsule, medium-dose Xifeng Capsule and CBZ used alone (P<0.05).

Conclusion: Xifeng Capsule used alone or combined with CBZ can effectively inhibit MRP1 expression in hippocampus and cortex of epileptic rats.

Key words: epilepsy, drugs, Chinese herbal, pilocarpine, multidrug resistance-associated protein 1, rats

Table 1

Effects of Xifeng Capsule and CBZ on SRS attack in rats with lithium-pilocarpine-induced epilepsy ($\bar{x}$±s)"

Group n Times of SRS
Model 9 35.7±4.2
Low-dose Xifeng Capsule 10 26.6±2.7△△
Medium-dose Xifeng Capsule 10 21.1±2.0△△▲□
High-dose Xifeng Capsule 10 13.5±3.7△△▲
20 mg CBZ 10 17.0±3.9△△□
High-dose Xifeng Capsule plus 20 mg CBZ 10 7.9±3.2△△▲□■
High-dose Xifeng Capsule plus 10 mg CBZ 10 5.5±2.1△△▲□■

Figure 1

Expression of MRP1 in cerebral cortex of rats observed by immunohistochemical method (Light microscopy, ×400) A: Normal control group; B: Model group. C: Low-dose Xifeng Capsule group; D: Medium-dose Xifeng Capsule group; E: High-dose Xifeng Capsule group; F: CBZ group; G: High-dose Xifeng Capsule plus 20 mg CBZ group; H: High-dose Xifeng Capsule plus 10 mg CBZ group. CBZ: carbamazepine; MRP1: multidrug resistance-associated protein 1. "

Figure 2

Expression of MRP1 in hippocampus of rats observed by immunohistochemical method (Light microscopy, ×400) A: Normal control group; B: Model group. C: Low-dose Xifeng Capsule group; D: Medium-dose Xifeng Capsule group; E: High-dose Xifeng Capsule group; F: CBZ group; G: High-dose Xifeng Capsule plus 20 mg CBZ group; H: High-dose Xifeng Capsule plus 10 mg CBZ group. CBZ: carbamazepine; MRP1: multidrug resistance-associated protein 1. "

Table 2

Effects of Xifeng Capsule and CBZ on distribution of MRP1 in rats with lithium-pilocarpine-induced epilepsy ($\bar{x}$±s)"

Group n Expression of MRP1
Cerebral cortex CA1 region CA3 region Dentate gyrus
Normal control 6 0.33±0.18 0.16±0.07 1.48±0.35 2.00±0.36
Model 6 4.00±0.82* 11.75±2.43* 10.25±6.97* 12.02±1.64*
Low-dose Xifeng Capsule 6 3.37±0.39* 8.45±1.02*△ 7.07±1.82* 10.72±1.97*
Medium-dose Xifeng Capsule 6 3.07±0.41*△□☆ 4.82±2.22*△□☆ 6.97±0.81*□☆ 10.07±0.89*△□☆
High-dose Xifeng Capsule 6 2.28±0.28*△▲ 1.85±0.45*△▲ 3.05±0.46*△▲ 4.70±0.91*△▲
20 mg CBZ 6 2.53±0.86*△▲ 2.60±0.87*△ 3.02±0.26*△ 6.10±0.72*△□
High-dose Xifeng Capsule plus 10 mg CBZ 6 2.02±0.67*△▲ 1.55±0.61*△▲■ 2.32±0.35*△▲□■☆ 3.10±0.49*△▲□■
High-dose Xifeng Capsule plus 20 mg CBZ 6 1.17±0.74*△▲□■ 1.05±0.27*△▲□■ 1.90±0.15*△▲□■ 2.60±0.54*△▲□■
[1] Shen DL, Wang XF. Clinical epileptology.2nd ed[M]. Shanghai: Shanghai Scientific and Technical Publishers, 2007,403
沈鼎列, 王学峰 . 临床癫痫学.第2版[M]. 上海: 上海科学技术出版社, 2007,403
[2] Li R, Huang YG . P-glycoprotei and intractable epilepsy[J]. Cu Zhong Yu Shen Jing Ji Bing, 2003,10(5):315-316
doi: 10.3969/j.issn.1007-0478.2003.05.025
李锐, 黄远桂 . P糖蛋白与难治性癫痫[J]. 卒中与神经疾病, 2003,10(5):315-316
doi: 10.3969/j.issn.1007-0478.2003.05.025
[3] Ma R, Li XM, Wei XW, Zhang XL, Yang CQ, Xiong J, Sun XH, Liu YZ . The study of Xifeng capsule on epilepsy tonic-clonic seizure in epileptic children[J]. Tianjin Zhong Yi Yao, 2003,20(2):87
马融, 李新民, 魏小维, 张喜莲, 杨常泉, 熊杰, 孙希焕, 刘玉珍 . 熄风胶囊治疗小儿癫痫强直-阵挛性发作的研究[J]. 天津中医药, 2003,20(2):87
[4] Wang BG, Liao WP, Luo AH, Sun WW, Su T . Comparison study of behavioral and histological changes between pilocarpine-induced epilepsy model and pentylenetetrazole kindling model in rats[J]. Lin Chuang He Shi Yan Yi Xue Za Zhi, 2006,5(3):197-199
王本国, 廖卫平, 罗爱华, 孙卫文, 苏涛 . 匹罗卡品癫痫模型及戊四唑点燃模型的行为学、组织学对比研究[J]. 临床和实验医学杂志, 2006,5(3):197-199
[5] Mazarati AM, Telegdy G . Effects of somatostatin and anti-somatostatin serum on picrotoxin-kindled seizures[J]. Neuropharmacology, 1992,31(8):793-797
doi: 10.1016/0028-3908(92)90043-O pmid: 1356254
[6] Xiong J, Ma R, Yang CQ, Zhang GZ . The effect of Xifeng Capsule on the activity of nitric oxide synthase in hippocampal subareas of epileptic mice[J]. Shanxi Zhong Yi, 2003,4(2):52-53
熊杰, 马融, 杨常泉, 张果忠 . 熄风胶囊对癫痫小鼠海马一氧化氮合酶活力的影响[J]. 山西中医, 2003,4(2):52-53
[7] Sisodiya SM, Lin WR, Harding BN, Squier MV, Thom M . Drug resistance in epilepsy: expression of drug resistance proteins in common causes of refractory epilepsy[J]. Brain, 2002,125(Pt 1):22-31
doi: 10.1093/brain/awf002
[8] Seegers U, Potschka H, Löscher W . Expression of the multidrug transporter P-glycoprotein in brain capillary endothelial cells and brain parenchyma of amygdala-kindled rats[J]. Epilepsia, 2002,43(7):675-684
doi: 10.1046/j.1528-1157.2002.33101.x
[9] Rizzi M, Caccia S, Guiso G, Richichi C, Gorter JA, Aronica E, Aliprandi M, Bagnati R, Fanelli R , D’Incalci M , Samanin R , Vezzani A .Limbic seizures induce P-glycoprotein in rodent brain: functional implications for pharmacoresistance[J]. J Neurosci, 2002,22(14):5833-5839
doi: 10.1523/JNEUROSCI.22-14-05833.2002
[1] Shu-yi Chen, Qun-wei Chen, Liu-mei Shou, Hong Pan, Shan-ming Ruan, Zhe-hao Liang, Qi-jin Shu. Stevens-Johnson syndrome/toxic epidermal necrolysis successfully treated with Chinese herbal medicine Pi-Yan-Ning: A case report. Journal of Integrative Medicine, 2021, 19(6): 555-560.
[2] Xin Yin, Shu-bin Cai, Lan-ting Tao, Lu-ming Chen, Zhong-de Zhang, Su-hong Xiao, Arthur Yin Fan, Xu Zou. Recovery of a patient with severe COVID-19 by acupuncture and Chinese herbal medicine adjuvant to standard care. Journal of Integrative Medicine, 2021, 19(5): 460-466.
[3] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[4] Qing-lan Wang, Yan-yan Tao, Hong-dong Xie, Cheng-hai Liu, Ping Liu. Fuzheng Huayu recipe, a traditional Chinese compound herbal medicine, attenuates renal interstitial fibrosis via targeting the miR-21/PTEN/AKT axis. Journal of Integrative Medicine, 2020, 18(6): 505-513.
[5] Ming-sheng Lyu, De-ying Li, Shao-zhong Zhou, Cheng-jun Ban, Jun Yan. Adult-onset Still's disease successfully treated with Chinese herbal medicine: A case report with 15-month follow-up. Journal of Integrative Medicine, 2020, 18(6): 530-534.
[6] Arthur Yin Fan, Sherman Gu, Sarah Faggert Alemi, Research Group for Evidence-based Chinese Medicine. Chinese herbal medicine for COVID-19: Current evidence with systematic review and meta-analysis. Journal of Integrative Medicine, 2020, 18(5): 385-394.
[7] Bin Lan, Jin-wen Ge, Shao-wu Cheng, Xi-long Zheng, Jun Liao, Chao He, Zheng-qing Rao, Guo-zuo Wang. Extract of Naotaifang, a compound Chinese herbal medicine, protects neuron ferroptosis induced by acute cerebral ischemia in rats. Journal of Integrative Medicine, 2020, 18(4): 344-350.
[8] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[9] Deng-hai Zhang, Kun-lun Wu, Xue Zhang, Sheng-qiong Deng, Bin Peng. In silico screening of Chinese herbal medicines with the potential to directly inhibit 2019 novel coronavirus. Journal of Integrative Medicine, 2020, 18(2): 152-158.
[10] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[11] Carola Analía Torres, Cristina Marisel Pérez Zamora, María Beatriz Nuñez, Ana María Gonzalez. In vitro antioxidant, antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae. Journal of Integrative Medicine, 2018, 16(4): 255-262.
[12] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[13] Shui-jie Shen, Yong-hong Zhang, Xiao-xia Gu, Shui-ju Jiang, Ling-jun Xu. Yangfei Kongliu Formula, a compound Chinese herbal medicine, combined with cisplatin, inhibits growth of lung cancer cells through transforming growth factor-β1 signaling pathway. Journal of Integrative Medicine, 2017, 15(3): 242-251.
[14] Liliana Zazueta-Beltrán, Lorena Medina-Aymerich, Nadia Estela Díaz-Triste, Aracely Evangelina Chávez-Piña, Gilberto Castañeda-Hernández, Leticia Cruz-Antonio. Evidence against the participation of a pharmacokinetic interaction in the protective effect of single-dose curcumin against gastrointestinal damage induced by indomethacin in rats. Journal of Integrative Medicine, 2017, 15(2): 151-157.
[15] Majid Nimrouzi, Mehdi Pasalar. Role of dietary and lifestyle modifications in reflex epilepsy based on traditional Persian medicine. Journal of Integrative Medicine, 2017, 15(1): 8-10.
Full text



[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278