固本化痰通脉方对痰阻血脉大鼠主动脉巨噬细胞和黏附分子表达的影响

doi:10.3736/jcim20060517

Abstract

Objective

To observe the effects of Guben Huatan Tongmai Recipe (GBHTTMR), a compound Chinese herbal recipe, on expressions of macrophages and cell adhesion molecules (CAMs) of aortic endothelia in rats with syndrome of phlegm blocking blood vessel, and to explore the pathogenesis of the phlegm-pathogen.

Methods

Fifty normal male Wistar rats, 7-week in age, were randomly divided into five groups: normal control group, untreated group, high-dose GBHTTMR-treated group, low-dose GBHTTMR-treated group and simvastatin-treated group, with 10 rats in each group. Syndrome of phlegm blocking blood vessel was induced in rats of the latter 4 groups by feeding the rats with high lipid diet. Levels of blood lipid were compared among the 5 groups. The expressions of macrophages and CAMs in aortic endothelia were tested by immunohistochemical staining method.

Results

The level of blood lipid, and the expressions of macrophages and CAMs showed statistical differences between the normal control group and the untreated group (P＜0.01), and between the untreated group and the low-, high-dose GBHTTMR-treated and simvastatin-treated groups as well (P＜0.05).

Conclusions

GBHTTMR can decrease the level of serum cholesterol and triglycerides, and increase the level of high density lipoprotein. It also can inhibit the expressions of macrophages, intercellular adhesion molecule-1, vascular cell adhesion molecule-1, P-selectin, and E-selectin.

Key words: Phlegm zheng, Simvastatin, Metabolism of blood lipid, Macrophage, cell adhesion molecules, Rats

CLC Number:

R289.5

Ping Wang, Fan Guo, Song-lin Liu, Dai-zhi Tian, Xiao-juan Zou, Fang-ping Cheng. Effects of Guben Hutan Tongmai Recipe on expressions of macrophages and cell adhesion molecules of aortic endothelia in rats with syndrome of phlegm blocking blood vessel. Journal of Chinese Integrative Medicine, 2006, 4(5): 522-525.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060517

http://www.jcimjournal.com/EN/Y2006/V4/I5/522

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Figures/Tables 4

Table 1

Table 2

Figure 1

Table 3

References 5

[1]	金惠铭, 卢建, 殷莲华.细胞分子病理生理学(第1版)[M].郑州: 郑州大学出版社,2002,(第1版):125
[2]	郝群, 李大金, 朱影, 等.补肾宁心方对单核-内皮细胞在动脉粥样硬化中相互作用的影响[J].中国老年学杂志,2004,24(4):337-339 doi: 10.3969/j.issn.1005-9202.2004.04.026
[3]	Davies MJ, Gordon JL, Gearing AJ, et al.The expression of the adhesion molecules ICAM-1, VCAM-1, PECAM, and E-selectin in human atherosclerosis[J].J Pathol,1993,171(3):223-229
[4]	王平, 田代志, 张茂林, 等.肺虚痰阻大鼠巨噬细胞功能和黏附分子CD11b的表达研究[J].中医药学刊,2004,22(7):1167, 1195 doi: 10.3969/j.issn.1673-7717.2004.07.005
[5]	Woolf N.Pathology of atherosclerosis[J].Br Med Bull,1990,46(4):960-985

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Group	n	TC	TG	HDL-C
Normal control	10	1.94±0.75	0.52±0.24	1.28±0.50
Untreated	10	5.83±0.56**	2.65±0.68**	0.24±0.08**
Low-dose GBHTTMR-treated	10	4.31±0.90^△△□□	1.92±0.76^△□	0.42±0.25^△□□
High-dose GBHTTMR-treated	10	3.46±0.59^△△▲	1.03±0.60^△△▲▲	0.74±0.22^△△▲▲
Simvastatin-treated	10	3.12±0.72^△△	1.25±0.50^△△	0.86±0.10^△△

Group	n	OD value of macrophages
Normal control	10	0.060±0.007
Untreated	10	0.426±0.013**
Low-dose GBHTTMR-treated	10	0.366±0.036^△△
High-dose GBHTTMR-treated	10	0.228±0.013^{△△▲▲□□}
Simvastatin-treated	10	0.365±0.021^△△

Group	n	OD value
Group	n	ICAM-1	VCAM-1	E-selectin	P-selectin
Normal control	10	0.091±0.005	0.087±0.002	0.084±0.006	0.073±0.003
Untreated	10	0.567±0.045*	0.325±0.051*	0.406±0.024*	0.419±0.029*
Low-dose GBHTTMR-treated	10	0.523±0.039*^△	0.301±0.048*	0.410±0.013*	0.406±0.038*
High-dose GBHTTMR-treated	10	0.334±0.088*^△△	0.174±0.030*^△△	0.290±0.052*^△△	0.296±0.021*^△△
Simvastatin-treated	10	0.532±0.010*^△	0.336±0.022*	0.398±0.043*	0.432±0.005*

Effects of Guben Hutan Tongmai Recipe on expressions of macrophages and cell adhesion molecules of aortic endothelia in rats with syndrome of phlegm blocking blood vessel

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