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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (6): 601-605.doi: 10.3736/jcim20060612

• Original Experimental Research • Previous Articles     Next Articles

Effects of Tongluo Recipe on experimental diabetic peripheral neuropathy in rats

De-gang Zhang1,Ying Zhao1,Pei-jin Xia2,Xiao-qun Huang1,Zhi-min Liu2,Hui Zhou1   

  1. 1. Department of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
    2. Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Online:2006-11-30 Published:2006-11-15

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Objective

To investigate the effects of Tongluo Recipe (TLR), a compound Chinese herbal medicine, on experimental diabetic peripheral neuropathy in rats.

Methods

Diabetes was induced by streptozocin in rats. Eight weeks later, the diabetic rats were assigned to 3 groups according to different body weights: untreated group, low-dose TLR-treated group and high-dose TLR-treated group. Eight normal rats were assigned to normal control group. Systemic and sciatic nerve intrinsic antioxidant enzyme activities, lipid peroxide levels, hemorheology and sciatic nerve conduction velocity and ultrastructure changes were evaluated.

Results

Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were lower, and lipid peroxide levels were higher in plasma and sciatic nerve of the untreated group than those in the normal control group (P＜0.05). Blood glucose and viscosity in the untreated group were higher than those in the normal control group. Sciatic nerve showed demyelination changes and decrease of conduction velocity. Vasa nervorum expanded and erythrocyte deposited in the capillary in the untreated group. TLR obviously improved sciatic nerve conduction velocity and ameliorated morphology changes, decreased blood viscosity, improved SOD and GSH-Px activities.

Conclusion

TLR can obviously improve sciatic nerve function and ameliorate morphology changes in rats with diabetic peripheral neuropathy, probably through depleting free radical production, improving endothelial cell function and ameliorating hemorheology changes.

Key words: Diabetes mellitus, Peripheral nerves, Sciatic nerve, Hemorheology, Rats

CLC Number: 

  • R587.1

Table 1

Body weight and blood glucose of rats in four groups（?x±s）"

GroupnBody weight (g)Blood glucose (mmol/L)
8 weeks16 weeks8 weeks16 weeks
Normal control8330.25±30.56612.50±56.765.15±0.815.16±0.68
Untreated8283.00±37.94*233.00±25.20**21.35±3.19**30.11±2.65**
Low-dose TLR-treated8/7286.75±60.19*257.88±69.83**24.78±3.43**30.27±2.62**
High-dose TLR-treated8283.75±47.74*252.88±49.06**23.06±5.04**29.08±2.95**

Table 2

MNCV of rats in four groups（ヌ±S, m/s）"

GroupnMNCV
8 weeks16 weeks
Normal control850.93±3.7651.64±3.42
Untreated843.32±3.18**36.51±2.49**
Low-dose TLR-treated8/740.49±2.90**42.41±2.57**△△
High-dose TLR-treated841.98±3.05**45.48±2.38**△△

Figure 1

Ultrastructure of sciatic nerve in 4 groupsA: Normal control group (×3000). The structure is clear. Myelin sheath is in integrity and microfilaments line up in order. B: Untreated group (×3000). Morphous of myelinated nerve fiber is irregular, swelling and deformed, and layers are separated or disappeared. C: Low-dose TLR-treated group (×2000). Myelin sheath of sciatic nerve is abnormal, the densities of layers on myelin sheath are uneven and rarefaction, but the damage is slighter than the untreated group. D: High-dose TLR-treated group (×2500). The structure of myelin sheath and microfilament line up clearly, and there is rarefaction in part myelin sheath."

Figure 2

Ultrastructure of vasa nervorum in 4 groupsA: Normal control group (×4000). The structure is normal, and blood capillary is easy and smooth. B: Untreated group (×4000). Gelatinous fiber surrounding vessels is lightly hyperplasia. Erythrocytes deposited in blood capillary, and lumens are obstructed. C: Low-dose TLR-treated group (×4000). A small erythrocyte is deposited in blood capillary, and blood capillary is easy and smooth. D: High-dose TLR-treated group (×4000). A small erythrocyte is deposited in blood capillary, and blood capillary is easy and smooth."

Table 3

Blood viscosity and plasma viscosity of rats in four groups（?x±s, mPa·s）"

GroupnBlood viscosity
200 /s30 /s3 /sPlasma viscosity
Normal control84.70±0.425.61±0.6910.59±0.621.36±0.27
Untreated86.82±0.798.13±1.0115.69±1.771.40±0.14
Low-dose TLR-treated75.70±0.65**△6.96±1.02**△14.14±1.67**1.49±0.33
High-dose TLR-treated85.28±0.56*△△6.61±0.87*△△12.69±1.75**△△1.43±0.27

Table 4

Activities of plasma SOD, GSH-Px and MDA level of rats in four groups（ヌ±S）"

GroupnSOD (kU/L)GSH-Px (kU/L)MDA (μmol/L)
Normal control8465.09±46.13817.87±106.125.88±0.79
Untreated8372.92±35.11613.88±94.089.61±1.45
Low-dose TLR-treated7469.88±55.56**765.53±106.89*7.42±0.70**
High-dose TLR-treated8486.59±61.26**831.81±90.58**6.87±1.56**

Table 5

Activities of sciatic nerve SOD, GSH-Px and MDA level of rats in four groups（ヌ±S）"

GroupnSOD (U/mg prot)GSH-Px (U/mg prot)MDA (nmol/mg prot)
Normal control813.60±1.8528.36±3.803.80±1.08
Untreated89.81±1.2522.63±4.625.55±1.11
Low-dose TLR-treated711.70±1.69*36.04±5.35**3.31±0.62**
High-dose TLR-treated812.78±1.75**38.26±9.45**3.21±0.75**
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