Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (6): 606-610.doi: 10.3736/jcim20060613

• Original Experimental Research • Previous Articles     Next Articles

Study of Huoxue Qianyang Granules in revising the left ventricular hypertrophy of spontaneous hypertension rats

Duan Zhou1,Mei-fang Xiao2,Rong Hu1   

  1. 1. Department of Cardiovascular Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Department of Traditional Chinese Internal Medicine, Central Hospital of Huangpu District, Shanghai 200002, China
  • Online:2006-11-30 Published:2006-11-15


To study the mechanism of Huoxue Qianyang Granule (HXQYG), a traditional Chinese compound medicine, in revising the left ventricular hypertrophy of hypertension.


Spontaneous hypertension rats (SHR) were randomly divided into seven groups: untreated group, Songling Xuemaikang (SLXMK)-treated group, captopril-treated group, high-, medium- and low-dose HXQYG-treated groups, and normal control group. The systolic blood pressure (SBP) and left ventricular mass index (LVMI) were measured. The content of angiotensin Ⅱ (AngⅡ) in left ventricular tissue was determined by radioimmunoassay. The expressions of angiotensin converting enzyme (ACE) protein and mRNA in left ventricular tissue were analyzed separately by immunohistochemical method and RT-PCR.


(1) SBP and LVMI were higher in the untreated group than those in the normal control group, and they were lower in the high- and medium-dose HXQYG-treated groups than those in the untreated group, but higher than those in the captopril-treated group, and without significant difference as compared to those in the SLXMK-treated group. (2) The content of AngⅡ and expressions of ACE protein and mRNA in the left ventricular tissue in the untreated group were higher than those in the normal control group, and they were lower in the HXQYG-treated groups than those in the untreated group, but higher than those in the captopril-treated group, and without significant difference as compared to those in the SLXMK-treated group.


HXQYG can reverse the left ventricular hypertrophy of SHR, which may be due to decreasing the amount of AngⅡ and expressions of ACE protein and mRNA in the left ventricular tissue.

Key words: Hypertension, Left ventricular hypertrophy, Angiotensin Ⅱ, Angiotensin converting enzyme, Rats

CLC Number: 

  • R322.1+1

Table 1

Results of SBP of rats in 7 groups (ヌ±S, mm Hg)"

Group n The first SBP The last SBP
Normal control 9 133±2* 133±2*
Untreated 9 193±5 214±4
Captopril-treated 9 194±7 168±4**
SLXMK-treated 9 194±9 201±4*
High-dose HXQYG-treated 9 193±5 193±6*
Medium-dose HXQYG-treated 9 194±7 201±3*
Low-dose HXQYG-treated 9 194±6 207±4*

Table 2

Results of LVMI of rats in 7 groups(ヌ±S, mg/kg)"

Group n LVMI
Normal control 12 2.14±0.22**
Untreated 12 2.73±0.11
Captopril-treated 13 2.45±0.11**
SLXMK-treated 13 2.62±0.15*△△
High-dose HXQY-treated 10 2.58±0.05*△△
Medium-dose HXQY-treated 11 2.58±0.11*△△
Low-dose HXQY-treated 10 2.65±0.08△△

Table 3

Content of AngⅡ and expressions of ACE protein and mRNA in left ventricle of rats in 7 groups(ヌ±S)"

Group Content of AngⅡ
n=11, pg/100 mg)
ACE protein integral optical
density (n=7)
ACE mRNA/β-actin
Normal control 1343±62* 13.61±2.28**△△ 0.39±0.18*
Untreated 1408±41 26.88±2.99 0.86±0.30
Captopril-treated 1316±45** 15.41±4.10** 0.57±0.13*
SLXMK-treated 1349±97 20.67±5.16**△△ 0.59±0.22*
High-dose HXQY-treated 1341±47* 18.17±1.46** 0.59±0.17*
Medium-dose HXQY-treated 1379±39 19.94±3.32** 0.57±0.27*
Low-dose HXQY-treated 1376±59 23.28±2.39△△ 0.73±0.37

Figure 1

Expression of ACE protein in left ventricle of rats (SABC, ×200) ACE mostly expressed in vascular endothelia cells and myocardial matrix cells, few in plasma and membrane of myocardial cells. The nigger-brown granules are indication of ACE (Red arrowhead). A: Normal control group, few expression of ACE; B: Untreated group, high expression of ACE; C: captopril-treated group, low expression of ACE ; D: High-dose HXQYG-treated group."

Figure 2

Electrophoretogram of ACE cDNAM: Marker; A: High-dose HXQYG-treated group; B: Medium-dose HXQYG-treated group; C: Low-dose HXQYG-treated group; D: Captopril-treated group; E: SLXMK-treated group; F: Untreated group; W: Normal control group. ACE: 317 bp; β-actin: 169 bp."

[1] Krizanova O, Kiss A, Zacikova L , et al. Nitric oxide synthase mRNA levels correlate with gene expression of angiotensin II type-1 but not type-2 receptors, renin or angiotensin converting enzyme in selected brain areas[J]. Physiol Res, 2001,50(5):473-480
[2] 周端, 苑素云, 顾仁樾 , 等. 活血潜阳胶囊改善高血压病血管重建的临床与实验研究[J]. 中国医药学报, 2001,16(4):310-312
doi: 10.3969/j.issn.1673-1727.2001.04.024
[3] Harrap SB, Dominiczak AF, Fraser R , et al. Plasma angiotensin II, predisposition to hypertension, and left ventricular size in healthy young adults[J]. Circulation, 1996,93(6):1148-1154
[4] Tokuda K, Kai H, Kuwahara F , et al. Pressure-independent effects of angiotensin II on hypertensive myocardial fibrosis[J]. Hypertension, 2004,43(2):499-503
doi: 10.1161/01.HYP.0000111831.50834.93 pmid: 14699000
[5] 余振球, 马长生, 赵连友 , 等. 实用高血压学(第2版)[M]. 北京: 科学出版社, 2000,( 第2版):66-69
[6] Schlaich MP, Kaye DM, Lambert E , et al. Relation between cardiac sympathetic activity and hypertensive left ventricular hypertrophy[J]. Circulation, 2003,108(5):560-565
[7] Klingbeil AU, Schneider M, Martus P , et al. A meta-analysis of the effects of treatment on left ventricular mass in essential hypertension[J]. Am J Med, 2003,115(1):41-46
doi: 10.1016/S0002-9343(03)00158-X pmid: 12867233
[8] Tanaka Y, Nagai M, Date T , et al. Effects of bradykinin on cardiovascular remodeling in renovascular Hypertensive Rats[J]. Hypertens Res, 2004,27(11):865-875
doi: 10.1291/hypres.27.865 pmid: 15824469
[9] Sayegh F, Topouchian J, Hlawaty M , et al. Regression of left ventricular hypertrophy with moexipril, an angiotensin-converting enzyme inhibitor, in hypertensive patients[J]. Am J Ther, 2005,12(1):3-8
[10] 符德玉, 罗海明, 黄蔚 , 等. 活血潜阳方治疗血瘀、阳亢证高血压病患者左心室肥厚的临床研究[J]. 上海中医药杂志, 2003,37(12):6-9
doi: 10.3969/j.issn.1007-1334.2003.12.003
[11] 肖梅芳 . 周端应用活血潜阳法治疗高血压病经验[J]. 上海中医药杂志, 2004,38(4):12-13
[12] 周端, 符德玉, 顾仁樾 . 活血潜阳胶囊治疗高血压病的临床与实验研究[J]. 上海中医药杂志, 2000,34(4):22-25
doi: 10.3969/j.issn.1007-1334.2000.04.008
[13] 符德玉, 周端, 顾仁樾 , 等. 活血潜阳胶囊治疗高血压病(血瘀、阳亢证)临床研究[J]. 中成药, 1999,2l(9):464-466
[14] 周端, 符德玉, 顾仁樾 . 活血潜镇胶囊对自发性高血压大鼠心肌组织ATⅡ、ET和血浆NO含量的影响[J]. 上海中医药大学上海市中医药研究院学报, 1998,12(2):70-72
[1] Shuai Chen, Jin Hu, Deng-cheng Lu, Hong-yi Liu, Shan-shan Wei. Metabolomic characteristics of spontaneously hypertensive rats under chronic stress and the treatment effect of Danzhi Xiaoyao Powder, a traditional Chinese medicine formula. Journal of Integrative Medicine, 2022, 20(1): 73-82.
[2] Rakhi Chakraborty, Swarnendu Roy. Angiotensin-converting enzyme inhibitors from plants: A review of their diversity, modes of action, prospects, and concerns in the management of diabetes-centric complications. Journal of Integrative Medicine, 2021, 19(6): 478-492.
[3] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[4] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[5] Pranay Soni, Rajesh Choudhary, Surendra H. Bodakhe. Effects of a novel isoflavonoid from the stem bark of Alstonia scholaris against fructose-induced experimental cataract. Journal of Integrative Medicine, 2019, 17(5): 374-382.
[6] Sonia Chew Wen Phang, Uma Devi Palanisamy, Khalid Abdul Kadir. Effects of geraniin (rambutan rind extract) on blood pressure and metabolic parameters in rats fed high-fat diet. Journal of Integrative Medicine, 2019, 17(2): 100-106.
[7] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[8] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[9] Nathalia Gomes Ribeiro de Moura, Ivan Cordovil, Arthurde Sá Ferreira. Traditional Chinese medicine wrist pulse-taking is associated with pulse waveform analysis and hemodynamics in hypertension. Journal of Integrative Medicine, 2016, 14(2): 100-113.
[10] Nouran A. Aleyeidi, Khaled S. Aseri, Shadia M. Matbouli, Albaraa A. Sulaiamani, Sumayyah A. Kobeisy. Effects of wet-cupping on blood pressure in hypertensive patients: A randomized controlled trial. Journal of Integrative Medicine, 2015, 13(6): 391-399.
[11] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[12] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[13] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[14] Ya Yuwen, Yu-qi Liu, Yan-ping Wang, Jin-gang Dai, Da-sheng Liu, Yue-xi Wang, Xue-jie Han. The add-on effect of a Chinese herbal formula for patients with resistant hypertension: Study protocol for a pilot cohort study. Journal of Integrative Medicine, 2015, 13(2): 122-128.
[15] Vamsi Reddy, Arvind Sridhar, Roberto F. Machado, Jiwang Chen. High sodium causes hypertension: Evidence from clinical trials and animal experiments. Journal of Integrative Medicine, 2015, 13(1): 1-8.
Full text



[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239