[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (6): 634-638.doi: 10.3736/jcim20060619

• Original Experimental Research • Previous Articles     Next Articles

Effects of Yiweining Recipe on expressions of metalloproteinase-2 and cyclooxygenase-2 mRNAs in ectopic endometrium of rats with endometriosis

Fan Qu1,Jue Zhou2,Dong-Xia Yang1,Wen-guang Ma1,Bao-zhang Ma1   

  1. 1. Department of Gynecology and Obstetrics, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China
    2. College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China
  • Online:2006-11-30 Published:2006-11-15

RichHTML

0

PDF (PC)

161

Cited

6

Like

Objective

To explore the effects of Yiweining Recipe (YWNR), a compound Chinese herbal medicine, on expressions of metalloproteinase-2 (MMP-2) and cyclooxygenase-2 (COX-2) mRNAs in rats with endometriosis (EM).

Methods

Operational self-transplantation was applied in establishing the rat models. Detection of MMP-2 and COX-2 mRNAs was conducted with hybridization in situ.

Results

There were significant differences in the expressions of MMP-2 and COX-2 mRNAs between the untreated group and the high-dose YWNR-treated group. YWNR could reduce the expressions of MMP-2 and COX-2 mRNAs.

Conclusion

YWNR can treat EM through reducing the positive expressions of MMP-2 and COX-2 mRNAs.

Key words: Endometriosis, Matrix metalloproteinase-2, Cyclooxygenase-2, Rats

CLC Number: 

  • R71

"

GroupnNumber of cases that exhibits various staining conditions
of the positive cells representing the positive expression of MMP-2 mRNA		Score
–++++++
Normal control925209**
Untreated9022521
High-dose YWNR-treated9243010*
Low-dose YWNR-treated9152112
Danazol-treated9233112

"

GroupnNumber of cases that exhibits various staining conditions
of the positive cells representing the positive expression of COX-2 mRNA		Score
–++++++
Normal control962104**
Untreated9023420
High-dose YWNR-treated935107*
Low-dose YWNR-treated9242111**
Danazol-treated9151112**
[1] Chishima F, Hayakawa S, Sugita K, et al.Increased expression of cyclooxygenase-2 in local lesions of endometriosis patients[J].Am J Reprod Immunol,2002,48(1):50-56
[2] Jones RL, Kelly RW, Critchley HO.Chemokine and cyclooxygenase-2 expression in human endometrium coincides with leukocyte accumulation[J].Hum Reprod,1997,12(6):1300-1306
[3] Matsuzaki S, Canis M, Pouly JL, et al.Cyclooxygenase-2 expression in deep endometriosis and matched eutopic endometrium[J].Fertil Steril,2004,82(5):1309-1315
doi: 10.1016/j.fertnstert.2004.03.059 pmid: 15533352
[4] Ota H, Igarashi S, Sasaki M, et al.Distribution of cyclooxygenase-2 in eutopic and ectopic endometrium in endometriosis and adenomyosis[J].Hum Reprod,2001,16(3):561-566
[5] Huang HF, Hong LH, Tan Y, et al.Matrix metalloproteinase 2 is associated with changes in steroid hormones in the sera and peritoneal fluid of patients with endometriosis[J].Fertil Steril,2004,81(5):1235-1239
doi: 10.1016/j.fertnstert.2003.10.027
[6] Togawa D, Koshino T, Saito T, et al.Highly activated matrix metalloproteinase-2 secreted from clones of metastatic lung nodules of nude mice injected with human fibrosarcoma HT1080[J].Cancer Lett,1999,146(1):25-33
[7] Rivilis I, Milkiewicz M, Boyd P, et al.Differential involvement of MMP-2 and VEGF during muscle stretch-versus shear stress-induced angiogenesis[J].Am J Physiol Heart Circ Physiol,2002,283(4):H1430-H1438
[8] Curry TE Jr, Osteen KG.Cyclic changes in the matrix metalloproteinase system in the ovary and uterus[J].Biol Reprod,2001,64(5):1285-1296
[9] Chung HW, Lee JY, Moon HS, et al.Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium[J].Fertil Steril,2002,78(4):787-795
doi: 10.1016/S0015-0282(02)03322-8
[10] Boyd RS, Balkwill FR.MMP-2 release and activation in ovarian carcinoma: the role of fibroblasts[J].Br J Cancer,1999,80(3-4):315-321
doi: 10.1038/sj.bjc.6690357 pmid: 2362343
[11] Hla T, Neilson K.Human cyclooxygenase-2 cDNA[J].Proc Natl Acad Sci U S A,1992,89(16):7384-7388
[12] Appleby SB, Ristimaki A, Neilson K, et al.Structure of the human cyclo-oxygenase-2 gene[J].Biochem J,1994,302(Pt 3):723-727
doi: 10.1006/bbrc.1994.2334
[13] Smith SK, Kelly RW.The release of PGF2 and PGE2 from separated cells of human endometrium and decidua[J].Prostagland Leukot Essent Fatty Acids,1988,33(2):91-96
doi: 10.1016/0952-3278(88)90146-9 pmid: 3174721
[14] Chishima F, Hayakawa S, Sugita K, et al.Increased expression of cyclooxygenase-2 in local lesions of endometriosis patients[J].Am J Reprod Immunol,2002,48(1):50-56
[15] Wu MH, Sun HS, Lin CC, et al.Distinct mechanisms regulate cyclooxygenase-1 and -2 in peritoneal macrophages of women with and without endometriosis[J].Mol Hum Reprod,2002,8(12):1103-1110
[16] Bartley J, Mechsner S, Beutler C, et al.COX-2-expression in extragenital endometriosis lesions as a novel therapeutical approach?[J].Zentralbl Gynakol,2003,125(7-8):252-255
doi: 10.1055/s-2003-42279 pmid: 14505259
[17] Fagotti A, Ferrandina G, Fanfani F, et al.Analysis of cyclooxygenase-2(COX-2) expression in different sites of endometriosis and correlation with clinico-pathological parameters[J].Hum Reprod,2004,19(2):393-397
[18] De Leon FD, Vijayakumar R, Brown M, et al.Peritoneal fluid volume, estrogen, progesterone, prostaglandin, and epidermal growth factor concentrations in patients with and without endometriosis[J].Obstet Gynecol,1986,68(2):189-194
[19] Gebel HM, Braun DP, Tambur A, et al.Spontaneous apoptosis of endometrial tissue is impaired in women with endometriosis[J].Fertil Steril,1998,69(6):1042-1047
doi: 10.1016/S0015-0282(98)00073-9 pmid: 9627290
[20] Dmowski WP, Ding J, Shen J, et al.Apoptosis in endometrial glandular and stromal cells in women with and without endometriosis[J].Hum Reprod,2001,16(9):1802-1808
doi: 10.1093/humrep/16.9.1802 pmid: 11527879
[21] Imai A, Takagi A, Tamaya T.Gonadotropin-releasing hormone analog repairs reduced endometrial cell apoptosis in endometriosis in vitro[J].Am J Obstet Gynecol,2000,182(5):1142-1146
[22] Sivula A, Talvensaari-Mattila A, Lundin J, et al.Association of cyclooxygenase-2 and matrix metalloproteinase-2 expression in human breast cancer[J].Breast Cancer Res Treat,2005,89(3):215-220
[23] Siironen P, Ristimaki A, Nordling S, et al.Expression of COX-2 is increased with age in papillary thyroid cancer[J].Histopathology,2004,44(5):490-497
doi: 10.1111/j.1365-2559.2004.01880 pmid: 15139997
[24] Yu CQ, Shi SF, Liu YH, et al.Primary culture and morphologic observation of eutopic and ectopic endometrial cells from patients with endometriosis[J].Zhong Xi Yi Jie He Xue Bao,2006,4(2):189-193
[1] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[2] Lawrence A. Olatunji, Taofeek O. Usman, Joseph O. Adebayo, Victoria A. Olatunji . Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats. Journal of Chinese Integrative Medicine, 2012, 10(9): 1049-1055.
[3] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[4] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[5] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[6] Karuna A. Talpate, Uma A. Bhosale, Mandar R. Zambare. Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 939-947.
[7] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[8] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[9] Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[10] Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[11] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[12] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[13] Ling Xu, Qiao-ling Zhou, Jie Han, Dan-ying Zhang, Hui-juan Dong, Zai-long Cai, Chao-qin Yu, Dong-xia Zhai . Effects of Chinese herbal medicine Neiyi Recipe-medicated serum on angiopoiesis of endometriosis in the chick chorioallantoic membrane model. Journal of Chinese Integrative Medicine, 2012, 10(7): 800-806.
[14] Feng Xin-ying, Zhu Min, Zhang Qi-qi, Chen Yi-ping, Li Wen-wei. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. Journal of Chinese Integrative Medicine, 2012, 10(7): 777-783.
[15] Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[an error occurred while processing this directive]