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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (2): 160-164.doi: 10.3736/jcim20070212

• Original Experimental Research • Previous Articles     Next Articles

Effects of Yiqi Kaimi Recipe on gastrointestinal motility and neuropeptides in rats with colonic slow transit constipation

Chun-mei He, Jin-gen Lu, Yong-qing Cao   

  1. Department of Anorectal Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Online:2007-03-31 Published:2007-03-15

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Objective: To observe the effects of Yiqi Kaimi Recipe (YQKMR), a traditional Chinese compound medicine, on gastrointestinal motility and neuropeptides in rats with colonic slow transit constipation.

Methods: Eighty specific pathogen free rats were included, and there were sixty rats with slow transit constipation (STC) and twenty normal rats. Sixty rats with STC were randomly divided into untreated group, cisapride-treated group and YQKMR-treated group, and twenty normal rats were allocated to normal control group. Percentage of carbon propelling, contraction frequency and amplitude of the muscle segments in vitro, and expressions of nitric oxide synthase-1 (NOS1) and substance P proteins were observed.

Results: Compared with the normal control group, the percentage of carbon propelling, and the contraction frequency and amplitude of the muscle segments in the untreated group decreased obviously (P＜0.05). The percentage of carbon propelling, and the contraction frequency and amplitude of the muscle segments in the YQKMR-treated group and cisapride-treated group were higher than those in the untreated group (P＜0.05). The expression of NOS1 protein in the untreated group was higher than that in the normal control group, YQKMR-treated group and cisapride-treated group. However, there were no significant differences between the YQKMR-treated group and the normal control group (P>0.05). The expression of substance P protein in the untreated group was lower than that in the normal control group, and there was no significant difference between the YQKMR-treated group and the normal control group too (P＞0.05).

Conclusion: Yiqi Kaimi Recipe could promote colon motility by increasing the contraction frequency and amplitude of the smooth muscle, and this may be due to its effect of regulating the expressions of NOS1 and substance P proteins in the enteric plexus.

Key words: colonic disease, functional, constipation, herbal preparations, gastrointestinal motility, neuropeptides, rats

CLC Number: 

  • R574.62

Table 1

Comparison of percentage of carbon propelling in 4 groups ($\bar{x}±S$, %)"

GroupnPercentage of carbon propelling
Normal control group1058.56±3.25
Untreated group1035.91±1.40△
Cisapride treated-group1055.16±2.47*△
YQKMR treated-group1057.30±1.87*

Table 2

Comparison of contraction frequency and amplitude of the muscle segments in 4 groups ($\bar{x}±S$)"

GroupnFrequency (contraction/min)Amplitude (mV)
Ileocecal junction
Normal control group1028.50±1.670.96±0.05
Untreated group1021.40±2.07△0.44±0.05△
Cisapride treated-group1027.20±1.48*△0.81±0.08*△
YQKMR treated-group1026.70±2.30*△0.87±0.04*△
Ascending colon
Normal control group1028.00±1.580.72±0.06
Untreated group1020.20±2.49△0.35±0.05△
Cisapride treated-group1027.00±2.12*0.69±0.07*
YQKMR treated-group1027.60±3.08*0.72±0.05*
Descending colon
Normal control group1021.60±1.820.51±0.09
Untreated group1016.30±3.39△0.24±0.07△
Cisapride treated-group1020.40±2.07*△0.40±0.03*△
YQKMR treated-group1020.10±3.05*△0.41±0.03*△

Figure 1

Contraction frequency and amplitude of smooth muscle segment of ascending colon of the rats in 4 groups A: Normal control group; B: Untreated group; C: YQKMR treated-group; D: Cisapride treated-group."

Figure 2

Expression of NOS1 protein in colon wall tissue of the rats (SABC,10×10) A: Normal control group; B: Untreated group; C: YQKMR treated-group; D: Cisapride treated-group."

Figure 3

Expression of SP in colon wall tissue of the rats (SABC, 10×20)A: Normal control group; B: Untreated group; C: YQKMR treatedgroup; D: Cisapride treatedgroup."

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