益气开秘方对结肠慢输型便秘大鼠肠动力和肠神经肽的影响

doi:10.3736/jcim20070212

Abstract

Objective: To observe the effects of Yiqi Kaimi Recipe (YQKMR), a traditional Chinese compound medicine, on gastrointestinal motility and neuropeptides in rats with colonic slow transit constipation.

Methods: Eighty specific pathogen free rats were included, and there were sixty rats with slow transit constipation (STC) and twenty normal rats. Sixty rats with STC were randomly divided into untreated group, cisapride-treated group and YQKMR-treated group, and twenty normal rats were allocated to normal control group. Percentage of carbon propelling, contraction frequency and amplitude of the muscle segments in vitro, and expressions of nitric oxide synthase-1 (NOS1) and substance P proteins were observed.

Results: Compared with the normal control group, the percentage of carbon propelling, and the contraction frequency and amplitude of the muscle segments in the untreated group decreased obviously (P＜0.05). The percentage of carbon propelling, and the contraction frequency and amplitude of the muscle segments in the YQKMR-treated group and cisapride-treated group were higher than those in the untreated group (P＜0.05). The expression of NOS1 protein in the untreated group was higher than that in the normal control group, YQKMR-treated group and cisapride-treated group. However, there were no significant differences between the YQKMR-treated group and the normal control group (P>0.05). The expression of substance P protein in the untreated group was lower than that in the normal control group, and there was no significant difference between the YQKMR-treated group and the normal control group too (P＞0.05).

Conclusion: Yiqi Kaimi Recipe could promote colon motility by increasing the contraction frequency and amplitude of the smooth muscle, and this may be due to its effect of regulating the expressions of NOS1 and substance P proteins in the enteric plexus.

Key words: colonic disease, functional, constipation, herbal preparations, gastrointestinal motility, neuropeptides, rats

CLC Number:

R574.62

Chun-mei He, Jin-gen Lu, Yong-qing Cao. Effects of Yiqi Kaimi Recipe on gastrointestinal motility and neuropeptides in rats with colonic slow transit constipation. Journal of Chinese Integrative Medicine, 2007, 5(2): 160-164.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20070212

http://www.jcimjournal.com/EN/Y2007/V5/I2/160

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References 7

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Group	n	Percentage of carbon propelling
Normal control group	10	58.56±3.25
Untreated group	10	35.91±1.40^△
Cisapride treated-group	10	55.16±2.47*^△
YQKMR treated-group	10	57.30±1.87*

Group	n	Frequency (contraction/min)	Amplitude (mV)
Ileocecal junction
Normal control group	10	28.50±1.67	0.96±0.05
Untreated group	10	21.40±2.07^△	0.44±0.05^△
Cisapride treated-group	10	27.20±1.48*^△	0.81±0.08*^△
YQKMR treated-group	10	26.70±2.30*^△	0.87±0.04*^△
Ascending colon
Normal control group	10	28.00±1.58	0.72±0.06
Untreated group	10	20.20±2.49^△	0.35±0.05^△
Cisapride treated-group	10	27.00±2.12*	0.69±0.07*
YQKMR treated-group	10	27.60±3.08*	0.72±0.05*
Descending colon
Normal control group	10	21.60±1.82	0.51±0.09
Untreated group	10	16.30±3.39^△	0.24±0.07^△
Cisapride treated-group	10	20.40±2.07*^△	0.40±0.03*^△
YQKMR treated-group	10	20.10±3.05*^△	0.41±0.03*^△

Effects of Yiqi Kaimi Recipe on gastrointestinal motility and neuropeptides in rats with colonic slow transit constipation

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