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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 526-530.doi: 10.3736/jcim20070510

• Original Experimental Research • Previous Articles     Next Articles

Effects of Wenyang Chubi Decoction on connective tissue growth factor and collagen-Ⅰ in a mouse model of scleroderma

Xiao-ning Yan, Jie Feng, Wen-bin Li, Rong Cui, Bin-jun Sui   

  1. Department of Dermatology , Second Hospital of Xi＇an Jiaotong University , Xi＇an , Shannxi Province 710004 , China
  • Online:2007-09-20 Published:2007-09-15

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Objective: To study the effects of Wenyang Chubi Decoction (WYCBD), a compound traditional Chinese herbal medicine, on connective tissue growth factor (CTGF) and collagen-Ⅰ (COL-Ⅰ) in a mouse model of scleroderma.Methods: Scleroderma was induced in BALB/c mice by daily local injection of bleomycin for three weeks and the mice were randomly divided into untreated, WYCBD-treated and normal saline (NS) treated groups, with another group of BALB/c mice as normal control. WYCBD and NS were given orally for one month respectively. Histopathology in the skin and lungs of the mice were examined. The CTGF and COL-Ⅰ expressions in the skin or skin lesions were detected by immunohistochemical Elivision assay.Results: The expression levels of CTGF and COL-Ⅰ in the untreated group were significantly higher than those in the normal control group (P＜0.05). Compared with the NS-treated group, the WYCBD-treated group had significant improvement in the skin and lung histopathology and remarkably decreased expression levels of CTGF and COL-Ⅰ (P＜0.05).Conclusion: Scleroderma mice showed high expressions of CTGF and COL-Ⅰin the skin. WYCBD had the effects of decreasing the CTGF and COL-Ⅰ expressions and improving the skin fibrosis.

Key words: scleroderma, drugs, Chinese herbal, experimental animal models, bleomycin, connective tissue, growth factors, collagen type Ⅰ, mice

CLC Number: 

  • R593.25

Figure 1

Morphology of skin tissue in different groups of mice (HE, ×10) A: Normal control group; B: Untreated group; C: NS-treated group; D: WYCBD-treated group."

Figure 2

Morphology of lung tissue in different groups of mice (HE, ×10) A: Normal control group; B: Untreated group; C: NS-treated group; D: WYCBD-treated group."

Table 1

Depth of dermis in different groups of mice (ヌ±S)"

Group n Depth of dermis
Normal control 8 134.37±89.61
Untreated 8 217.78±27.25*
NS-treated 12 204.83±42.32*
WYCBD-treated 12 149.14±32.81△

Table 2

Immunohistochemical parameters of CTGF and COL-Ⅰin skin of mice in different groups (ヌ±S)"

Group n CTGF COL-Ⅰ
Normal control 8 42 256.74±2 447.86 28 456.50±3 112.62
Untreated 8 164 003.65±7 824.88* 159 948.20±8 965.22*
NS-treated 12 156 418.97±8 725.96* 149 894.68±7 963.87*
WYCBD-treated 12 64 896.79±7 264.83△ 78 659.72±2 764. 87△
[1] Yamamoto T, Takagawa S, Katayama I , et al. Animal model of sclerotic skin. I: Local injections of bleomycin induce sclerotic skin mimicking scleroderma[J]. J Invest Dermatol, 1999,112(4):456-462
[2] 朱鹭冰, 李明 . 博来霉素诱导硬皮病小鼠模型及其抗体测定的初步研究[J]. 中华皮肤科杂志, 2003,36(9):496-498
Zhu LB, Li M . Establishment and antibody verification of the animal model of sclerotic skin induced by bleomycin[J]. Zhonghua Pi Fu Ke Za Zhi, 2003,36(9):496-498
[3] 屠文震, 赵凯, 陆磊 . 建立硬皮病小鼠模型的实验研究[J]. 中华风湿病学杂志, 2003,7(8):478-481
Tu WZ, Zhao K, Lu L . Experimental study of establishing mouse model for scleroderma[J]. Zhonghua Feng Shi Bing Xue Za Zhi, 2003,7(8):478-481
[4] 章元沛, 苏怀德 . 药理学实验(第2版)[M]. 北京: 人民卫生出版社, 1996,( 第2版):238-240
Zhang YP, Su HD. Pharmacology experiment(2nd ed)[M]. Beijing: People's Medical Publishing House, 1996,( 2nd ed):238-240
[5] Bradham DM, Igarashi A, Potter RL , et al. Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10[J]. J Cell Biol, 1991,114(6):1285-1294
[6] Igarashi A, Nashiro K, Kikuchi K , et al. Significant correlation between connective tissue growth factor gene expression and skin sclerosis in tissue sections from patients with systemic sclerosis[J]. J Invest Dermatol, 1995,105(2):280-284
[7] Querfeld C, Eckes B, Huerkamp C , et al. Expression of TGF-beta 1, -beta 2 and -beta 3 in localized and systemic scleroderma[J]. J Dermatol Sci, 1999,21(1):13-22
[8] Margotta V, Capogreco M . Soft tissue pathologies of the oral cavity[J]. Minerva Stomatol, 2003,52(1-2):47-51
pmid: 12686915
[9] Pasco S, Ramont L, Maquart FX , et al. Biological effects of collagen I and IV peptides[J]. J Soc Biol, 2003,197(1):31-39
pmid: 12868264
[10] Stumpf M, Cao W, Klinge U , et al. Collagen distribution and expression of matrix metalloproteinases l and 13 in patients with anastomotic leakage after large-bowel surgery[J]. Langenbecks Arch Surg, 2002,386(7):502-506
[11] Cutroneo KR . How is type 1 procollagen synjournal regulated at the gene level during tissue fibrosis[J]. J Cell Biochem, 2003,90(1):1-5
[12] 李明, 王强, 胡东艳 , 等. 温阳补肾中药对系统性硬皮病患者皮肤成纤维细胞增殖的影响[J]. 中国麻风皮肤病杂志, 2000,16(2):106-107
Li M, Wang Q, Hu DY , et al. Effects of traditional Chinese herbs for warming yang and invigorating the kidney on skin fibroblast proliferation in patients with systemic scleroderma[J]. Zhongguo Ma Feng Pi Fu Bing Za Zhi, 2000,16(2):106-107
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