平喘方对哮喘模型小鼠肺组织炎症及γ干扰素和白细胞介素4的影响

doi:10.3736/jcim20120712

Abstract

Objective: To observe the changes of interferon-γ (IFN-γ) and interleukin-4 (IL-4), factors related to T helper 1/T helper 2 (Th1/Th2), in airway inflammation in an asthmatic model of mice and the effects of Pingchuan Formula (PCF), a compound traditional Chinese herbal medicine.
Methods: Eighty BALB/c mice were randomly divided into normal group, model group, dexamethasone group and PCF group, with 20 mice in each group. For inducing asthma, the BALB/c mice were sensitized by intraperitoneal injection of mixed ovalbumin, and then activated by inhaling ovalbumin. After 2 weeks of sensitizing, asthmatic mice were intragastrically administered with the drugs once a day for four weeks. Bronchoalveolar lavage fluid (BALF) and lung tissue were collected respectively after 7-day activation or 2 weeks after activation. The general histological changes were observed by hematoxylin and eosin staining and collagen staining, meanwhile the levels of IFN-γ and IL-4 in BALF were detected by enzyme-linked immunosorbent assay.
Results: After 7 d of activation, compared with the normal group, significant decrease in IFN-γ and increase in IL-4 were detected in the model group; compared with the model group, IL-4 level of the two treated groups was decreased and the IFN-γ/IL-4 ratio was increased significantly; no significant difference was found between the two treated groups. Two weeks after activation, compared with the normal group, IFN-γ was decreased, IL-4 was increased, and the IFN-γ/IL-4 ratio was decreased in the model group; compared with the model group, IL-4 level in the dexamethasone group was significantly decreased and IFN-γ/IL-4 ratio was significantly elevated, while the level of IL-4 in the PCF group was significantly decreased, the level of IFN-γ was increased simultaneously, and the IFN-γ/IL-4 ratio was significantly elevated. There was no significant difference between the two treated groups in IFN-γ and IL-4 levels, nor in the IFN-γ/IL-4 ratio. The area of collagen fiber in airway wall epithelium in the model group was smaller than that in the normal group, and those in the treated groups were lower than that in the model group; there was no significant difference between the two treated groups.
Conclusion: PCF has regulating function in correcting the imbalance of Th1/Th2 so as to improve airway inflammation. The therapeutic effects of PCF are basically similar to dexamethasone.

Key words: bronchial asthma, drugs, Chinese herbal, interferon-γ, interleukin-4, Th1 cells, Th2 cells, mice

Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20120712

http://www.jcimjournal.com/EN/Y2012/V10/I7/807

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[1]	Finotto S, Glimcher L . T cell directives for transcriptional regulation in asthma[J]. Springer Semin Immunopathol, 2004,25(3-4):281-294 doi: 10.1007/s00281-003-0143-1 pmid: 15007632
[2]	Liu LG, Hou AC, Fang HQ, Hao YY . Changes of T helper cells in peripheral blood of children with bronchial asthma[J]. Shi Yong Er Ke Lin Chuang Za Zhi, 2006,21(4):217-218 doi: 10.3969/j.issn.1003-515X.2006.04.013
	刘力戈, 侯安存, 方惠秋, 郝艳艳 . 支气管哮喘患儿血T辅助淋巴细胞的变化[J]. 实用儿科临床杂志, 2006,21(4):217-218 doi: 10.3969/j.issn.1003-515X.2006.04.013
[3]	Szabo SJ, Kim ST, Costa GL, Zhang X, Fathman CG, Glimcher LH . A novel transcription factor, T-bet, directs Th1 lineage commitment[J]. Cell, 2000,100(6):655-669 doi: 10.1016/S0092-8674(00)80702-3 pmid: 10761931
[4]	Chakir H, Wang H, Lefebvre DE, Webb J, Scott FW . T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3[J]. J Immunol Methods, 2003,278(1-2):157-169 doi: 10.1016/S0022-1759(03)00200-X
[5]	Zhu HH, Yu JE, Zhang XF, Chen YP, Pang HF, Wang GH, Guan Y, Wu J . Experimental effects of “Pingchuan Decoction”on bronchial asthma macrophage inflammatory protein-1α and expression of CD86 in mice[J]. Shanghai Zhong Yi Yao Za Zhi, 2008,42(9):58-62 doi: 10.3969/j.issn.1007-1334.2008.09.025
	朱慧华, 虞坚尔, 张晓峰, 陈燕萍, 庞惠芳, 王国华, 管宇, 吴杰 . 平喘方对支气管哮喘模型小鼠巨噬细胞炎性蛋白-1α水平及CD86分子表达的实验研究[J]. 上海中医药杂志, 2008,42(9):58-62 doi: 10.3969/j.issn.1007-1334.2008.09.025
[6]	Li YK. Experimental methodology of traditional Chinese medicine pharmacology.2nd ed[M]. Shanghai: Shanghai Scientific and Technical Publishers, 2006: 119-120
	李仪奎 . 中药药理实验方法学.第2版[M]. 上海: 上海科学技术出版社, 2006: 119-120
[7]	Sun JF. Methodology of experimental animals[M]. Beijing: People’s Medical Publishing House, 2002: 357
	孙敬方 . 动物实验方法学[M]. 北京: 人民卫生出版社, 2002: 357
[8]	Li YY. Modern research and treatment of phlegm diseases in traditional Chinese medicine[M]. Beijing: Academy Press, 2003: 298-360
	李以义 . 中医痰病的现代研究与治疗[M]. 北京: 学苑出版社, 2003: 298-360
[9]	Zeng CC, Zhou JL . Theory of the formation and study feature in phlegm syndrome[J]. Zhong Yi Yan Jiu, 2003,16(6):4-6
	曾常春, 周俊亮 . 痰证理论的形成及其研究特点[J]. 中医研究, 2003,16(6):4-6
[10]	Xu JZ, Wu YG, Li MH. TCM combined with Western medicine in treatment of asthma[M]. Beijing: People’s Medical Publishing House, 2000: 19
	许建中, 吴银根, 李明华 . 中西医结合哮喘病学[M]. 北京: 人民卫生出版社, 2000: 19

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Group	n	Collagen area (mm²)		Optical density (J/mm²)		Integrated optical density (J)
Group	n	Activation 7 d	2 weeks after activation	Activation 7 d	2 weeks after activation	Activation 7 d	2 weeks after activation
Normal	10	480.71±214.58	527.56±230.23	0.13±0.03	0.16±0.02	64.24±35.14	84.85±40.46
Model	10	4 784.62± 2 336.15^▲▲	8 200.99± 3 741.15^▲	0.17±0.02^**	0.18±0.03	824.02± 403.88^▲▲	1 366.02± 525.78^▲▲
DXM	10	1 518.62± 649.04^▲▲□□	2 280.76± 811.85^▲▲□□	0.15±0.03^△	0.15±0.02	216.90± 76.33^▲▲□□	354.38± 145.03^▲▲□□
PCF	10	1 358.84± 251.40^▲▲□□	2 116.10± 822.23^▲▲□□	0.13±0.02^△△	0.16±0.02	177.07± 29.56^▲▲□□	321.75± 104.19^▲▲□□

Group	n	Interferon-γ (ng/L)		Iterleukin-4 (ng/L)		Interferon-γ/iterleukin-4
Group	n	Activation 7 d	2 weeks after activation	Activation 7 d	2 weeks after activation	Activation 7 d	2 weeks after activation
Normal	10	16.57±4.72	18.03±2.46	1.46±0.17	1.50±0.43	11.48±3.65	12.13±3.20
Model	10	10.11±5.56^**	14.04±2.14^**	2.13±0.55^**	3.12±0.78^**	4.89±3.20^**	4.80±1.59^**
DXM	10	12.41±2.06^*	14.53±1.74^**	1.65±0.56^△	1.73±1.03^△△	8.50±3.46^△	9.20±3.86^△△
PCF	10	14.35±3.98^△	16.23±2.60^△	1.57±0.33^△△	2.07±0.74^△△	9.68±4.02^△△	9.07±4.78^△

Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma

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