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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 526-530.doi: 10.3736/jcim20070510

• Original Experimental Research • Previous Articles     Next Articles

Effects of Wenyang Chubi Decoction on connective tissue growth factor and collagen-Ⅰ in a mouse model of scleroderma

Xiao-ning Yan, Jie Feng, Wen-bin Li, Rong Cui, Bin-jun Sui   

  1. Department of Dermatology , Second Hospital of Xi'an Jiaotong University , Xi'an , Shannxi Province 710004 , China
  • Online:2007-09-20 Published:2007-09-15

Objective: To study the effects of Wenyang Chubi Decoction (WYCBD), a compound traditional Chinese herbal medicine, on connective tissue growth factor (CTGF) and collagen-Ⅰ (COL-Ⅰ) in a mouse model of scleroderma.Methods: Scleroderma was induced in BALB/c mice by daily local injection of bleomycin for three weeks and the mice were randomly divided into untreated, WYCBD-treated and normal saline (NS) treated groups, with another group of BALB/c mice as normal control. WYCBD and NS were given orally for one month respectively. Histopathology in the skin and lungs of the mice were examined. The CTGF and COL-Ⅰ expressions in the skin or skin lesions were detected by immunohistochemical Elivision assay.Results: The expression levels of CTGF and COL-Ⅰ in the untreated group were significantly higher than those in the normal control group (P<0.05). Compared with the NS-treated group, the WYCBD-treated group had significant improvement in the skin and lung histopathology and remarkably decreased expression levels of CTGF and COL-Ⅰ (P<0.05).Conclusion: Scleroderma mice showed high expressions of CTGF and COL-Ⅰin the skin. WYCBD had the effects of decreasing the CTGF and COL-Ⅰ expressions and improving the skin fibrosis.

Key words: scleroderma, drugs, Chinese herbal, experimental animal models, bleomycin, connective tissue, growth factors, collagen type Ⅰ, mice

CLC Number: 

  • R593.25

Figure 1

Morphology of skin tissue in different groups of mice (HE, ×10) A: Normal control group; B: Untreated group; C: NS-treated group; D: WYCBD-treated group."

Figure 2

Morphology of lung tissue in different groups of mice (HE, ×10) A: Normal control group; B: Untreated group; C: NS-treated group; D: WYCBD-treated group."

Table 1

Depth of dermis in different groups of mice (ヌ±S)"

Group n Depth of dermis
Normal control 8 134.37±89.61
Untreated 8 217.78±27.25*
NS-treated 12 204.83±42.32*
WYCBD-treated 12 149.14±32.81

Table 2

Immunohistochemical parameters of CTGF and COL-Ⅰin skin of mice in different groups (ヌ±S)"

Group n CTGF COL-Ⅰ
Normal control 8 42 256.74±2 447.86 28 456.50±3 112.62
Untreated 8 164 003.65±7 824.88* 159 948.20±8 965.22*
NS-treated 12 156 418.97±8 725.96* 149 894.68±7 963.87*
WYCBD-treated 12 64 896.79±7 264.83 78 659.72±2 764. 87
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