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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (3): 278-282.doi: 10.3736/jcim20080312

• Original Experimental Research • Previous Articles     Next Articles

Effects of ginsenoside and berberine on secretion of immunosuppressive cytokines in lung carcinoma cell line PG

Yu Hao(), Ping Wang, Jun Wu, Quan-ying Qiu   

  1. Department of Pathogenic Organism and Immunology, School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2007-07-25 Online:2008-03-20 Published:2008-03-15
  • Contact: Y Hao E-mail:yuhao64@sina.com

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Objective: To observe the effects of ginsenoside (Gs) and berberine (Ber), two kinds of active components of traditional Chinese herbal medicine, on transforming growth factor-beta1 (TGF-β1) and prostaglandin E2 (PGE2) in PG cells.Methods: Co-culture system of human lung carcinoma cell line PG and human T lymphocyte cell line Jurkat was established. PG cells were treated with Gs (100 μg/ml) and Ber (10 μg/ml) for twenty-four hours, and then cocultured with Jurkat cells. After 24-hour coculture, the state of Jurkat cells was observed with inverted microscope. The viable count of Jurkat cells was detected by trypan blue staining after 6- and 24-hour coculture, and the apoptosis of Jurkat cells was evaluated by flow cytometry. PG cells were treated with 100, 50, 25 μg/ml Gs and 10, 5, 2.5 μg/ml Ber respectively, and the content of TGF-β1 and PGE2 in PG cells was detected by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) method.Results: After coculture with PG cells treated with Gs and Ber, the number of Jurkat cells was less than blank control group, and the apoptosis rates of Jurkat cells in Gs- and Ber-treated groups were higher than blank control group. Gs and Ber could promote the secretion of TGF-β1 in PG cells, but could not change the level of PGE2.Conclusion: Gs and Ber can promote the growth inhibition and apoptosis of Jurkat cells induced by PG cells, which may be related to the up-regulation of Gs and Ber on TGF-β1 secretion in PG cells.

Key words: ginsenosides, berberine, suppressor factors, immunologic, transforming growth factor (31, prostaglandins E

CLC Number: 

  • R734.2

Figure 1

Growth status of Jurkat cells cocultured with PG cells treated with Gs and Ber for 24 h (Inverted microscope, ×100) A: Jurkat cells group (uncocultured control); B: Blank control group (cocultured with untreated PG cells); C: Gs-treated group (cocultured with Gs-treated PG cells); D: Ber-treated group (cocultured with Ber-treated PG cells)."

Table 1

Viable cell count of Jurkat cells after coculture with PG cells ($\bar{x}$±s,105/ml)"

Group n Viable cell count of Jurkat cells
After 6 h coculture After 24 h coculture
Jurkat cells 3 3.0±0.2 5.4±0.3
Blank control 3 2.4±0.2△ 3.6±0.2△
Gs-treated 3 1.4±0.1**△△ 2.2±0.2**△△
Ber-treated 3 2.4±0.1△ 2.7±0.2**△△

Table 2

Apoptosis rate of Jurkat cells detected by flow cytometry ($\bar{x}$±s, %)"

Group n Early phase (Annexin-V-FITC) Middle-terminal phase ( PI ) Total apoptosis rate
Jurkat cells 3 5.57±1.20 4.67±0.57 10.24±1.49
Blank control 3 11.40±1.94△△ 36.69±2.29△△ 48.10±0.51△△
Gs-treated 3 15.79±0.54*△△ 40.40±1.81△△ 56.19±1.28**△△
Ber-treated 3 12.05±3.28△△ 40.03±3.26△△ 52.08±2.17*△△

Table 3

Content of TGF-β1 and PGE2 in PG cell culture supernatants ($\bar{x}$±s, ng/L)"

Group n TGF-β1 PGE2
Blank control 4 14.9±4.2 28.7±2.3
Gs1 (100 μg/ml)-treated 4 24.9±2.0* 28.9±11.3
Gs2 (50 μg/ml)-treated 4 16.0±3.5 18.2±6.8
Gs3 (25 μg/ml)-treated 4 20.4±2.4 19.1±8.5
Ber1 (10 μg/ml)-treated 4 21.0±1.2 28.9±6.1
Ber2 (5 μg/ml)-treated 4 30.4±4.4* 19.1±7.8
Ber3 (2.5 μg/ml)-treated 4 28.8±4.2* 24.3±1.1

Figure 2

Concentration of TGF-β1 in PG cells"

Figure 3

PG cell number detected by MTT method"

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