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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (10): 949-954.doi: 10.3736/jcim20101006

• Original Experimental Research • Previous Articles     Next Articles

Preventive effects of a sachet of Chinese herbs on influenza and its immune regulation

L Liu,XQ Yue,LN Wang,W Gu,HL Xin,GY Zheng,CQ Ling   

  1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2010-03-24 Accepted:2010-06-17 Online:2010-10-20 Published:2010-10-15

Objective

To study the clinical efficacy of Pihui Fanggan Sachet (PHFGS), a sachet of traditional Chinese herbs, in preventing influenza and its immune regulation on mice.
Methods

In clinical study, 239 children from Shanghai Baoshan Xubeihong Art Kindergarten were randomly divided into two groups according to different class; 118 children were treated with PHFGS for 45 days as treatment group and 121 children were as blank control. During the observation period, the incidence rate of influenza, the course of disease and the severity of symptoms were recorded. In experimental study, 40 Kunming mice were randomly divided into normal control group (normal mice treated or not treated with PHFGS), and immunocompromised group (immunocompromised mice treated or not treated with PHFGS). Immunocompromise was induced by intraperitoneal injection of 80 mg/kg cyclophosphamide for 3 days. Some mice in the normal control and immunocompromised groups were then treated with extracted solution of PHFGS through nasal cavity for one week. Spleen index, content of CD3 + T cells and CD4 + T cells, CD4 +/CD8 +ratio, activity of natural killer (NK) cells, serum level of interferon γ (INF-γ) and respiratory level of secretory immunoglobulin A (SIgA) were evaluated.
Results

The incidence rate of influenza in the treatment group was much lower than that in the blank control group (P<0.01), and the average course was shortened as compared with the control group (P<0.01). The fever, rhinocleisis, runny nose, and throat congestion in the treatment group were improved as compared with the blank control group (P<0.05, P<0.01). After injection of cyclophosphamide, the spleen index, content of CD3 +T cells and CD4 +T cells, CD4 +/CD8 + ratio, activity of NK cells, serum level of INF-γ and respiratory level of SIgA in the immunocompromised group were significantly lower than those in the normal control group (P<0.05 or P<0.01), which indicated the immunosuppression. After treated with PHFGS for one week, the spleen index and the respiratory level of SIgA in the immunocompromised group were improved significantly. Although the content of CD3 + T cells and CD4 + T cells, CD4 +/CD8 + ratio and the serum level of INF-γ were improved, the differences did not reach the significant level. No significant effects on immune function of normal mice were observed.
Conclusion

PHFGS can prevent influenza effectively by improving the immunity, especially the respiratory mucosal immune function.

Key words: Influenza, Prevention, Defense mechanisms, Prevalence, Immunologic markers, Mice

Table 1

Contents of T lymphocyte subsets in different groups"

Table 2

Levels of INF-γ, NK activity, spleen index and SIgA in different groups"

[1] Liu YH, Dong JC . Progress in pharmacotherapy of integrated traditional Chinese and Western medicine for virus infection in respiratory tract[J]. J Chin Integr Med, 2004,2(4):226-227
刘羽华, 董竞成 . 呼吸道病毒感染中西医药物治疗进展[J]. 中西医结合学报, 2004,2(4):226-227
[2] Hu J, Liu JP . Chinese medicinal herbs for influenza[J]. J Chin Integr Med, 2007,5(2):197
胡俊, 刘建平 . 中药治疗流行性感冒[J]. 中西医结合学报, 2007,5(2):197
[3] Society of Respiratory Diseases, Chinese Medical Association . Guidelines for the diagnosis and treatment of influenza(draft)[J]. Zhonghua Jie He He Hu Xi Za Zhi, 2002,25(2):66-68
中华医学会呼吸病学分会. 流行性感冒临床诊断和治疗指南(草案)[J]. 中华结核和呼吸杂志, 2002,25(2):66-68
[4] Ministry of Health of the People’s Republic of China. Guidelines for clinical research on Chinese new herbal medicines[M]. Beijing: China Medical Science and Technology Press,1993:
中华人民共和国卫生部. 中药新药临床研究指导原则[M]. 北京: 中国医药科技出版社,1993:
[5] Zhao YQ, Luo X, Chen DH, Yu MY, Yang ZR . Comparison of immunosuppression induced by different doses of cyclophosphamide in normal mice[J]. Mian Yi Xue Za Zhi, 2005,21(3):122-124
赵弋清, 罗霞, 陈东辉, 余梦瑶, 杨志荣 . 不同剂量环磷酰胺诱导正常小鼠免疫抑制的对比研究[J]. 免疫学杂志, 2005,21(3):122-124
[6] Yan DX, Yu XP, Shi KH, Song WB, Zhang HY, Wei JL . Discussion about treatment of severe acute respiratory syndrome based on syndrome differentiation[J]. J Chin Integr Med, 2004,2(4):241-244
颜德馨, 余小萍, 石克华, 宋文宝 . 张惠勇, 魏江磊 . 传染性非典型性肺炎的中医证治探讨[J]. 中西医结合学报, 2004,2(4):241-244
[7] Li JX .Experience of treating severe acute respiratory syndrome with traditional Chinese medicine[J].J Chin Integr Med, 2004, 2(1): 6, 29
doi: 10.3736/jcim20040103
李军祥 .中医药治疗传染性非典型肺炎体会[J].中西医结合学报, 2004, 2(1): 6, 29
doi: 10.3736/jcim20040103
[8] World Health Organization. Confirmation of H1N1 viruses resistant to Tamiflu[J]. Shi Jie Bo Lan, 2009,( 15):16
世界卫生组织. 确认对达菲具有耐药性的H1N1病毒[J]. 世界博览, 2009,( 15):16
[9] Zhang CJ, Wang SP, Chen HH, Chen Q, Guo HY, Zhang JR . Clinical study of wind-warm and pulmonary heat syndrome treated with integrated traditional Chinese and Western medicine[J]. J Chin Integr Med, 2005,3(2):108-110
张存钧, 王松坡, 陈怀红, 陈钦, 郭海英, 张镜人 . 中西医结合治疗风温肺热证临床观察[J]. 中西医结合学报, 2005,3(2):108-110
[10] Zhang WB, Jiang HL, Zhou W, Zhong YQ, Yang HM, Fu JJ, Mao B . Chinese medicine for acute upper respiratory tract infection: a systematic review of randomized controlled trials[J]. J Chin Integr Med, 2009,7(8):706-716
张文斌, 蒋红丽, 周维, 钟云青, 杨红梅, 付娟娟, 毛兵 . 中药治疗急性上呼吸道感染随机对照试验的系统评价[J]. 中西医结合学报, 2009,7(8):706-716
[11] Mei QX, Gao YQ . Current progress in chemistry and pharmacology of Folium Artemisiae Argyi[J]. Zhong Cheng Yao, 2006,28(7):1030-1032
梅全喜, 高玉桥 . 艾叶化学及药理研究进展[J]. 中成药, 2006,28(7):1030-1032
[12] Huang WD, Lu WQ . Current progress in research of Borneolum Synthcticum[J]. Zhongguo Yao Ye, 2008,17(4):64-66
黄卫东, 吕武清 . 冰片的研究进展[J]. 中国药业, 2008,17(4):64-66
[13] Zhang Y, Zhang JC, Chen Y, Yang MS, Cao H, Xiao PG . Current progress in pharmacognosy, chemistry, and pharmacology of Herba Pogostemonis[J]. Zhong Cao Yao, 2006,37(5):786-790
doi: 10.7501/j.issn.0253-2670.2006.5.329
张英, 张金超, 陈瑶, 杨梦苏, 曹晖, 肖培根 . 广藿香生药、化学及药理学的研究进展[J]. 中草药, 2006,37(5):786-790
doi: 10.7501/j.issn.0253-2670.2006.5.329
[14] Wei DZ, Ning SJ, Lin WX . Current progress in research of Herba Eupatorii Fortunei[J]. Shi Zhen Guo Yi Guo Yao, 2007,18(7):1782-1783
魏道智, 宁书菊, 林文雄 . 佩兰的研究进展[J]. 时珍国医国药, 2007,18(7):1782-1783
[15] Liu HJ, Jin RM . Current progress in research of essential oil of Herba Menthae Haplocalycis[J]. Shandong Zhong Yi Yao Da Xue Xue Bao, 2006,30(6):502-505
刘红杰, 金若敏 . 薄荷油研究进展[J]. 山东中医药大学学报, 2006,30(6):502-505
[16] Xu GY . Sialylsugar chains on cell surface in attacking process of influenza virus[J]. Hua Xue Tong Bao, 2000,63(6):31-34
doi: 10.3969/j.issn.0441-3776.2000.06.005
徐桂云 . 糖链在流感病毒侵袭细胞中的作用[J]. 化学通报, 2000,63(6):31-34
doi: 10.3969/j.issn.0441-3776.2000.06.005
[17] Yang YQ, Chen HP . Current progress in basal and clinical research of SIgA: a literature review[J]. Shanghai MianYi Xue Za Zhi, 1991,11(3):176
杨永清, 陈汉平 . SIgA的基础和临床研究进展:文献综述[J]. 上海免疫学杂志, 1991,11(3):176
[18] Wang DF, Gan ZW, Wang NJ, Cao JH . Study on the correlation between QDS and SIgA of nasal secretion in chronic rhinitis[J]. Zhongguo Zhong Xi Yi Jie He Er Bi Yan Hou Ke Za Zhi, 1999,7(4):161-162
王东方, 干祖望, 王宁皎, 曹济航 . 慢性鼻炎鼻分泌物SIgA含量与气虚相关性研究[J]. 中国中西医结合耳鼻咽喉科杂志, 1999,7(4):161-162
[19] Zhang ZJ. Viral respiratory infection and viral pneumonia of child[M]. Beijing: China Medical Science and Technology Press,1990: 4-8
张梓荆 . 小儿病毒性呼吸道感染与病毒性肺炎[M]. 北京: 中国医药科技出版社,1990: 4-8
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