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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (7): 720-724.doi: 10.3736/jcim20080712

• Original Experimental Research • Previous Articles     Next Articles

Scutellaria barbata extract enhances efficacy and reduces toxicity of chemotherapy in hepatoma H22-bearing mice

Zhi-jun Dai1(), Xi-jing Wang1, Zong-zheng Ji2, Zong-fang Li2, Wei Tang3, Hua-feng Kang1, Xiao-bin Ma1, Lei Liu1   

  1. 1. Department of Oncology,Second Hospital,School of Medicine,Xi’an Jiaotong University,Xi’an,Shannxi Province 710004, China;
    2. Department of General Surgery, Second Hospital, School of Medicine, Xi’an Jiaotong University,Xi’an,Shannxi Province 710004, China;
    3. Department of Life Science, Shannxi Normal University, Xi'an, Shannxi Province 710062, China
  • Received:2007-10-13 Online:2008-07-20 Published:2008-07-15
  • Contact: DAI Zhi-jun E-mail:dzj0911@126.com

Objective: To study the assistant effect of Scutellaria barbata extract (ESB) in 5-fluorouracil (5-FU) chemotherapy.
Methods: A mouse model of transplanted hepatoma H22 was used in this study to evaluate the synergic and attenuating effects of ESB in chemotherapy. Tumor inhibition rate, life span of mice and the toxicity of chemotherapy were observed. The body weight, tumor weight, thymus index and spleen index in H22-bearing mice were also measured. The phagocytotic function of macrophages was studied by observing phagocytiz-ation of peritoneal macrophages.
Results: The increase of body weight in 5-FU plus ESB groups was higher than that in 5-FU group, and the side effects such as anorexia, abdominal distention and athrepsy were relieved. Compared with untreated group, prolonged lifetime in 5-FU plus high-dose ESB group and 5-FU plus low-dose ESB group was improved. Life prolongation rates in 5-FU plus high-dose ESB group and 5-FU plus low-dose ESB group were 61.46% and 23.59% respectively. High-dose ESB, 5-FU, 5-FU plus low-dose ESB and 5-FU plus high-dose ESB could inhibit the tumor growth, and the tumor inhibition rates were 36.98%, 42.26%, 52.45% and 65.28%, respectively. Thymus index and spleen index were increased significantly in 5-FU plus low-dose ESB group and 5-FU plus high-dose ESB group. White blood cell (WBC) count was decreased obviously in 5-FU group, while the count of WBC was increased in 5-FU plus low-dose ESB group and 5-FU plus high-dose ESB group. The phagocytotic function of macrophages was also increased in 5-FU plus low-dose ESB group and 5-FU plus high-dose ESB group.
Conclusion: ESB can enhance the effect of chemotherapy, relieve the side effects and improve immune function of mice in chemotherapy. These results suggest that ESB, as a biochemical modulator to enhance the therapeutic effects, is useful in cancer chemotherapy.

Key words: Scutellaria barbata, liver cancer, immunity, chemotherapy, mice

CLC Number: 

  • R735.7

Table 1

Effect of ESB on body weight in H22 solid tumor-bearing mice ($\bar{x}$±s, g)"

Group n Body weight
Pre-treatment Post-treatment Variation of body weight
Normal control 10 20.03±1.94 24.12±2.02 4.09±2.32
Untreated 10 20.03±2.07 23.50±3.24 3.47±3.82
Low-dose ESB 10 20.00±1.94 24.52±1.82 4.52±3.93
High-dose ESB 10 20.01±2.10 25.15±2.13 5.14±3.76△▲
5-FU 10 19.96±2.03 20.74±1.86 0.78±1.93
5-FU plus low-dose ESB 10 20.00±2.13 22.46±1.92 2.46±1.62
5-FU plus high-dose ESB 10 19.98±2.05 24.33±2.05 4.35±3.25△▲

Table 2

Effects of ESB on life-prolonging rate and tumor inhibition rate in H22-bearing mice ($\bar{x}$±s )"

Group n Ascites tumor-bearing mice Solid tumor-bearing mice
Survival time (d) Life-prolonging rate (%) Tumor weight (g) Tumor inhibition rate (%)
Normal control 10
Untreated 10 16.32±4.92 2.65±1.12
Low-dose ESB 10 18.47±5.23 13.17 2.27±0.93 14.34
High-dose ESB 10 24.65±5.80△▲ 51.04 1.67±0.76 36.98
5-FU 10 13.26±4.15 -3.72 1.53±0.64 42.26
5-FU plus low-dose ESB 10 20.17±4.54 23.59 1.26±0.45 52.45
5-FU plus high-dose ESB 10 26.35±5.65△▲ 61.46 0.92±0.58△▲ 65.28

Table 3

Effects of ESB on thymus index, spleen index and WBC count in H22 solid tumor-bearing mice ($\bar{x}$±s )"

Group n Thymus index (mg/g) Spleen index (mg/g) White blood cell count (109/L)
Normal control 10 3.64±2.03 5.67±2.37 9.08±0.74
Untreated 10 2.47±0.68* 3.82±2.32* 8.78±0.68
Low-dose ESB 10 2.62±1.54 4.25±1.92 8.82±0.59
High-dose ESB 10 3.65±2.36△▲ 5.44±3.06△▲ 9.16±0.70
5-FU 10 1.43±0.56 2.92±1.73 5.15±0.52
5-FU plus low-dose ESB 10 2.55±1.32 3.96±1.65 7.62±0.64
5-FU plus high-dose ESB 10 3.16±2.15 4.72±2.02 8.47±0.58

Table 4

Effect of ESB on phagocytotic function of macrophages in H22 solid tumor-bearing mice ($\bar{x}$±s )"

Group n Phagocytizing rate (%) Phagocytizing index
Normal control 10 45.83±3.33 0.94±0.04
Untreated 10 38.52±3.16 0.73±0.04
Low-dose ESB 10 41.32±3.05 0.79±0.05
High-dose ESB 10 47.46±3.55△▲ 0.93±0.06△▲
5-FU 10 22.47±2.12 0.49±0.03
5-FU plus low-dose ESB 10 32.35±2.53 0.71±0.06
5-FU plus high-dose ESB 10 40.12±3.68 0.89±0.05
[1] Yin X, Zhou J, Jie C , et al. Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549[J]. Life Sci, 2004,75(18):2233-2244
doi: 10.1016/j.lfs.2004.05.015
[2] Lin JM, Liu Y, Luo RC . Inhibition activity of Scutellariae barbata extracts against human hepatocellular carcinoma cells[J]. Nan Fang Yi Ke Da Xue Xue Bao, 2006,26(5):591-593
doi: 10.3321/j.issn:1673-4254.2006.05.014
林敬明, 刘煜, 罗荣城 . 半枝莲抑制人肝癌QGY-7701细胞增殖研究[J]. 南方医科大学学报, 2006,26(5):591-593
doi: 10.3321/j.issn:1673-4254.2006.05.014
[3] Ye J, Xu XK, Zhou JW , et al. Prevention and treatment of herba Scutellariae barbatae compound on diethylnitrosamine induced hepatocarcinoma of rat[J]. Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi, 2002,10(2):67-80
doi: 10.3969/j.issn.1671-038X.2002.02.001
叶健, 徐锡坤, 周建伟 , 等. 半枝莲防治二乙基亚硝胺诱发大鼠肝癌的研究[J]. 中国中西医结合消化杂志, 2002,10(2):67-80
doi: 10.3969/j.issn.1671-038X.2002.02.001
[4] Chen Z, Wang P, Huang WX , et al. Experimental study on effects of Shengmai Injection: enhancing 5-FU anti-tumor efficacy and reducing its toxicity[J]. Zhong Xi Yi Jie He Xue Bao, 2005,3(6):476-479
陈震, 王鹏, 黄雯霞 , 等. 生脉注射液对5-FU增效减毒作用的实验研究[J]. 中西医结合学报, 2005,3(6):476-479
[5] Wu SL, Sun ZJ, Yu L , et al. Effect of resveratrol and in combination with 5-FU on murine liver cancer[J]. World J Gastroenterol, 2004,10(20):3048-3052
doi: 10.1111/j.1365-2036.2004.02175.x pmid: 15378791
[6] Cao Y, Xia QH, Meng H , et al. Antitumor and synergistic effect of Chinese medicine bushen huayu jiedu recipe and chemotherapy on transplanted animal hepatocarcinoma[J]. World J Gastroenterol, 2005,11(33):5218-5220
[7] Li XJ, Mao YX, Zhang HL , et al. Study on effects of anticancer and immunoregulation of Fuganchun 6 on hepatoma of mouse[J]. Zhongguo Zhong Yao Za Zhi, 2006,31(19):1622-1625
李秀娟, 毛宇湘, 张焕铃 , 等. 复肝春6号对小鼠肝癌的抑制作用及对荷瘤小鼠免疫功能的影响[J]. 中国中药杂志, 2006,31(19):1622-1625
[8] Liu MH, Zhang Z, Gu L , et al. Effects of powders of Wu Shi Bai Ye San(WSBRYS) for the tumor growth and immune function in tumor bearing mice[J]. Zhongguo Xin Yao Za Zhi, 2006,15(3):201-204
doi: 10.3321/j.issn:1003-3734.2006.03.009
刘明华, 章卓, 顾立 , 等. 伍氏百叶散对荷瘤小鼠肿瘤生长及免疫功能的影响[J]. 中国新药杂志, 2006,15(3):201-204
doi: 10.3321/j.issn:1003-3734.2006.03.009
[9] Wang G, Dong M, Liu XS , et al. Anti-tumor activity study of extract from Scutellaria barbata D. Don[J]. Xian Dai Zhong Xi Yi Jie He Za Zhi, 2004,13(9):1141-1142
doi: 10.3969/j.issn.1008-8849.2004.09.010
王刚, 董玫, 刘秀书 , 等. 半枝莲醇提物抗肿瘤活性的研究[J]. 现代中西医结合杂志, 2004,13(9):1141-1142
doi: 10.3969/j.issn.1008-8849.2004.09.010
[10] Wang WS, Zhou YW, Ye YH , et al. Studies on the flavonoids in herb from Scutellaria barbata[J]. Zhongguo Zhong Yao Za Zhi, 2004,29(10):957-959
doi: 10.3321/j.issn:1001-5302.2004.10.009
王文蜀, 周亚伟, 叶蕴华 , 等. 半枝莲中黄酮类化学成分研究[J]. 中国中药杂志, 2004,29(10):957-959
doi: 10.3321/j.issn:1001-5302.2004.10.009
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