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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (3): 289-293.doi: 10.3736/jcim20080314

• Original Experimental Research • Previous Articles     Next Articles

Amelioration effect of Zhenqing Capsule on peripheral neuropathy in type 1 diabetic rats

Xiu-ying Wen1(), Qin-hui Hu1, Fang Wang2   

  1. 1. Department of Integrated Traditional Chinese and Western Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei Province 430077, China
    2. Department of Senile Diseases, Mawangdui Geriatric Hospital, Changsha, Hunan Province 421001, China
  • Received:2007-07-27 Online:2008-03-20 Published:2008-03-15
  • Contact: XY Wen E-mail:Wenshowing@yahoo.com.cn

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Objective: To investigate the effects of Zhenqing Capsule (ZQC), a compound traditional Chinese herbal medicine, in treating type 1 diabetic rats with peripheral neuropathy.Methods: Type 1 diabetes was induced by caudal vein injection of high-dose streptozotocin in 30 male Wistar rats. The thirty diabetic rats were randomly divided into three groups: ZQC-treated group, untreated group and aminoguanidine-treated group. Another group of 10 rats was taken as normal control. After 10-week treatment, the changes of body weight and fasting plasma glucose level were measured, and the serum MDA level and the changes of neurological electrophysiology were analyzed. The samples of sciatic nerve in diabetic rats were taken for morphological observation.Results: The MDA level in type 1 diabetic rats was notably reduced in ZQC-treated group as compared with the untreated group (P<0.01). Compared with the untreated group, ZQC could improve the electrophysiology of sciatic nerve including conduction velocity (P<0.05), latency (P<0.01) and wave amplitude (P<0.05). The nerve myelin staining results showed that segmental demyelination of the nerve fibers in ZQC-treated group was not as serious as that in the untreated group.Conclusion: ZQC can obviously ameliorate the neurological electrophysiological function and the pathological changes of peripheral nerve in type 1 diabetic rats through the removal of free radical and resistance of lipid peroxidation.

Key words: Zhenqing Capsule, diabetes mellitus, type 1, diabetic neuropathies, malondialdehyde, rats

CLC Number: 

  • R587.25

Table 1

Body weight and fasting blood sugar level in different groups before and after treatment ($\bar{x}$±s )"

Group n Body weight (g) Fasting blood sugar (mmol/L)
Pre-treatment Post-treatment Pre-treatment Post-treatment
Normal control 9 143.43±11.28 334.20±16.69 5.05±0.95 5.12±0.93
Untreated 8 137.50±12.40 244.38 22.43** 19.06±2.30** 19.42±1.73**
Aminoguanidine-treated 8 139.50±15.21 248.75±33.57** 19.04±2.06** 19.29 2.02**
ZQC-treated 8 138.88±16.52 252.50±26.73**△ 18.87±2.18** 17.70±3.08**

Table 2

Serum MDA level in different groups ($\bar{x}$±s, μmol/L)"

Group n MDA level
Normal control 9 11.03±1.98
Untreated 8 21.67±2.11**
Aminoguanidine-treated 8 16.35±3.24**△△
ZQC-treated 8 15.58±3.68**△△

Table 3

Electrophysiology index in sciatic nerve in different groups ($\bar{x}$±s )"

Group n Conduction velocity (m/s) Latency (ms) Wave amplitude (mV)
Normal control 9 36.7±6.1 1.16±0.29 38.01±4.79
Untreated 8 22.1±5.9** 1.78±0.17** 28.25±3.54**
Aminoguanidine-treated 8 31.2±4.3*△△ 1.39±0.06*△△ 29.16±3.18**
ZQC-treated 8 29.5±5.2*△ 1.24±0.23△△ 32.37±4.00*△

Figure 1

Morphology characteristics of sciatic nerve in rats (Myelin staining, ×400) A: Normal control group; B: Untreated group; C: Aminoguanidine-treated group; D: ZQC-treated group."

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