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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (3): 294-297.doi: 10.3736/jcim20080315

• Original Experimental Research • Previous Articles     Next Articles

Preventive and therapeutic effects of Bushen Huoxue Recipe on autoimmune premature ovarian failure in mice

Li Dong(), Lin Jiang, Wei Meng, Nan-sun Zhu   

  1. Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2007-09-13 Online:2008-03-20 Published:2018-10-11
  • Contact: L Dong E-mail:dongli888@yahoo.com

Objective: To investigate the preventive effect of Bushen Huoxue Recipe (BSHXR), a compound traditional Chinese herbal medicine, on autoimmune premature ovarian failure (POF) in mice.Methods: Ovarian antigen was prepared with the ovarian tissue of female BALB/c mice. A mouse model of POF was established by immunization injection of the ovarian antigen of isotype female mice on multiple subcutaneous sites and two posterior soles. The POF mice were treated with BSHXR after the first and third immunization. The levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) in peripheral blood were detected by enzyme linked immunosorbent assay.Results: Lymphocyte infiltration was appeared in ovarian stroma of POF mice. The levels of FSH and LH were evaluated and the E2 level was decreased significantly (P<0.05). BSHXR could reduce the increased levels of FSH and LH, increase the level of E2 and the number of growing and mature follicles. The efficacy of early treatment was better than that of late treatment.Conclusion: BSHXR can recover ovarian function in POF mice mainly by regulating the indiscriminate hormone level, and BSHXR has preventive effect on autoimmune POF in mice.

Key words: ovarian failure, premature, herbal preparations, mice

CLC Number: 

  • R588.6

Figure 1

Morphology characteristics of ovarian tissue (Light microscope, ×40) A: Normal group; B: Untreated group; C: Prevention group; D: BSHXR-treated group."

Table 1

Levels of FSH, LH and E2 in mice in different groups ($\bar{x}$±s )"

Group n FSH (U/L) LH (U/L) E2 (ng/L)
Normal 15 1.6±0.12 1.03±0.14 38.6±4.4
Untreated 15 2.6±0.13* 1.85±0.09* 16.6±2.3*
Prevention 15 1.8±0.18 0.93±0.13 24.3±4.1*
BSHXR-treated 15 2.1±0.15* 1.15±0.18* 19.6±2.7*
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