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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (3): 294-297.doi: 10.3736/jcim20080315

• Original Experimental Research • Previous Articles     Next Articles

Preventive and therapeutic effects of Bushen Huoxue Recipe on autoimmune premature ovarian failure in mice

Li Dong(), Lin Jiang, Wei Meng, Nan-sun Zhu   

  1. Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2007-09-13 Online:2008-03-20 Published:2018-10-11
  • Contact: L Dong E-mail:dongli888@yahoo.com

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Objective: To investigate the preventive effect of Bushen Huoxue Recipe (BSHXR), a compound traditional Chinese herbal medicine, on autoimmune premature ovarian failure (POF) in mice.Methods: Ovarian antigen was prepared with the ovarian tissue of female BALB/c mice. A mouse model of POF was established by immunization injection of the ovarian antigen of isotype female mice on multiple subcutaneous sites and two posterior soles. The POF mice were treated with BSHXR after the first and third immunization. The levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) in peripheral blood were detected by enzyme linked immunosorbent assay.Results: Lymphocyte infiltration was appeared in ovarian stroma of POF mice. The levels of FSH and LH were evaluated and the E2 level was decreased significantly (P<0.05). BSHXR could reduce the increased levels of FSH and LH, increase the level of E2 and the number of growing and mature follicles. The efficacy of early treatment was better than that of late treatment.Conclusion: BSHXR can recover ovarian function in POF mice mainly by regulating the indiscriminate hormone level, and BSHXR has preventive effect on autoimmune POF in mice.

Key words: ovarian failure, premature, herbal preparations, mice

CLC Number: 

  • R588.6

Figure 1

Morphology characteristics of ovarian tissue (Light microscope, ×40) A: Normal group; B: Untreated group; C: Prevention group; D: BSHXR-treated group."

Table 1

Levels of FSH, LH and E2 in mice in different groups ($\bar{x}$±s )"

Group n FSH (U/L) LH (U/L) E2 (ng/L)
Normal 15 1.6±0.12 1.03±0.14 38.6±4.4
Untreated 15 2.6±0.13* 1.85±0.09* 16.6±2.3*
Prevention 15 1.8±0.18△ 0.93±0.13△ 24.3±4.1*△
BSHXR-treated 15 2.1±0.15*△ 1.15±0.18*△ 19.6±2.7*△
[1] Orshan SA, Furniss KK, Forst C , et al. The lived experience of premature ovarian failure[J]. J Obstet Gynecol Neonatal Nurs, 2001,30(2):202-208
doi: 10.1111/j.1552-6909.2001.tb01536.x pmid: 11308110
[2] Pasquali EA . The impact of premature menopause on women's experience of self[J]. J Holist Nurs, 1999,17(4):346-364
doi: 10.1177/089801019901700404 pmid: 10818847
[3] 闫洁, 王涛 . 卵巢早衰的病因及中西医结合治疗的研究[J]. 天津药学, 2006,18(3):66-68
doi: 10.3969/j.issn.1006-5687.2006.03.034
Yan J, Wang T . Study on premature ovary failure etiology and the treatment with combined traditional Chinese-Western medicine[J]. Tianjin Yao Xue, 2006,18(3):66-68
[4] Lin JH, Yan JH, Lin QD , et al. Determination of cellular and humoral immunity in mice of autoimmune ovarian failure[J]. Zhonghua Fu Chan Ke Za Zhi, 1998,33(12):735-736
doi: 10.3760/j.issn:0529-567X.1998.12.010
林建华, 严隽鸿, 林其德 , 等. 自身免疫性卵巢功能衰退小鼠体液免疫和细胞免疫的测定[J]. 中华妇产科杂志, 1999,33(12):735-736
doi: 10.3760/j.issn:0529-567X.1998.12.010
[5] Cheng BB, Zhu L, Li CZ , et al. Effect of Zuogui pill on reproductive endocrine of female Yin-deficiency mice[J]. Xian Dai Zhong Xi Yi Jie He Za Zhi, 2003,12(13):1362-1364
doi: 10.3969/j.issn.1008-8849.2003.13.008
程彬彬, 朱玲, 李长征 , 等. 左归丸对雌性小鼠阴虚模型生殖内分泌的影响[J]. 现代中西医结合杂志, 2003,12(13):1362-1364
doi: 10.3969/j.issn.1008-8849.2003.13.008
[6] Shen W, Yu CQ . Treating premature ovarian failure by reinforcing kidney[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(6):653-656
沈慰, 俞超芹 . 从肾论治卵巢早衰[J]. 中西医结合学报, 2006,4(6):653-656
[7] Yang ZW, Tan ZY, You ZL , et al. Treatment of polycystic ovary syndrome by tonifying kidney and promoting blood circulation[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(4):422-424
杨正望, 谈珍瑜, 尤昭玲 , 等. 补肾活血法治疗多囊卵巢综合征临床观察[J]. 中西医结合学报, 2006,4(4):422-424
[8] Cai LR, Li DJ, Sun XX , et al. Experimental study on preventive and therapeutic effect of Bushen Huoxue Recipe on autoimmune premature ovarian failure model[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2001,21(2):126-129
蔡立荣, 李大金, 孙晓溪 , 等. 补肾活血方对小鼠实验性卵巢早衰防治作用的研究[J]. 中国中西医结合杂志, 2001,21(2):126-129
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