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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (4): 361-365.doi: 10.3736/jcim20080407

• Original Experimental Research • Previous Articles     Next Articles

Effects of Bushen Huayu Qutan Recipe on local ovarian factors in androgen-sterilized rats

Zhen-zhi Wang, Chao-qin Yu()   

  1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2007-07-22 Online:2008-04-20 Published:2008-04-15
  • Contact: YU Chao-qin E-mail:chqyu81@gmail.com

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Objective: To explore the effects of Bushen Huayu Qutan Recipe (BHQR), a compound traditional Chinese herbal medicine, on local ovarian factors in androgen-sterilized rats (ASR).
Methods: One hundred and five 9-day-old SD rats were randomly divided into normal control group (15 rats) and ASR group (90 rats). ASR was induced by subcutaneous injection of testosterone propionate, and the rat model of ASR was established successfully in 30 rats in the ASR group. Thirty ASR rats were randomly divided into untreated group and BHQR-treated group. There were 15 rats in each group. The rats in BHQR-treated group were intragastrically administered with BHQR. The rats in the normal control and untreated groups were intragastrically administered with distilled water. They were all treated for 30 days. The levels of serum testosterone and insulin (INS) were measured by radioimmunoassay. The inhibin (INH) level, and the expressions of insulin-like growth factor-Ⅰ(IGF-Ⅰ) and vascular endothelial growth factor (VEGF) in ovary were determined by immunohistochemical method.
Results: None of the ASR ovulated and all the rats in the normal control group ovulated. The rate of ovulation in BHQR-treated group was 66.67%. The serum concentrations of testosterone and INS in the untreated group were significantly higher than those in the normal control group (P<0.05), and the serum concentrations of testosterone and INS in BHQR-treated group were decreased as compared with the untreated group (P<0.05). The levels of ovarian INH, IGF-Ⅰand VEGF in the untreated group were significantly higher than those in the normal control group (P<0.01), and the levels of ovarian INH, IGF-Ⅰand VEGF in BHQR-treated group were decreased as compared with the untreated group (P<0.01).
Conclusion: BHQR is an effective recipe in promoting ovulation, and the effects of BHQR on balancing the internal environment of ovary may be due to the reduction of serum INS level and decrease in the expressions of ovarian INH, IGF-Ⅰand VEGF.

Key words: Bushen Huayu Qutan Recipe, infertility, inhibins, insulin-like growth factor I, vascular endothelial growth factors, rats

CLC Number: 

  • R271.14

Table 1

Results of INS in oral glucose tolerance test in three groups ($\bar{x}$± s, μg/L)"

Group n INS level
0 min 30 min 60 min 120 min
Normal control 15 1.01±0.23 1.22±0.21 1.54±0.21 1.14±0.13
Untreated 15 1.77±0.44* 2.10±0.47* 2.27±0.67* 1.94±0.50*
BHQR-treated 15 1.36±0.33*△ 1.53±0.43*△ 1.77±0.42*△ 1.61±0.47*

Table 2

Expressions of ovarian INH, IGF-Ⅰand VEGF in three groups ($\bar{x}$±s )"

Group n INH IGF-Ⅰ VEGF
Normal control 15 0.50±0.11 1.70±0.12 0.90±0.15
Untreated 15 2.10±0.55** 3.22±0.72** 3.67±0.38**
BHQR -treated 15 1.06±0.14**△△ 1.81±0.55△△ 1.67±0.36**△△

Figure 1

Expressions of ovarian INH, IGF-Ⅰand VEGF in three groups (Immunohistochemical method, ×250) A: Expression of ovarian INH in normal control group; B: Expression of ovarian INH in untreated group; C: Expression of ovarian INH in BHQR-treated group; D: Expression of ovarian IGF-Ⅰ in normal control group; E: Expression of ovarian IGF-Ⅰ in untreated group; F: Expression of ovarian IGF-Ⅰ in BHQR-treated group; G: Expression of ovarian VEGF in normal control group; H: Expression of ovarian VEGF in untreated group; I: Expression of ovarian VEGF in BHQR-treated group."

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