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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (9): 934-938.doi: 10.3736/jcim20080911

• Original Experimental Research • Previous Articles     Next Articles

Bushen Rougan Recipe in prevention of hepatic fibrosis in rats induced by dimethylnitrosamine: a study on its preliminary mechanism

Bin Zhang1(), Ling-tai Wang2   

  1. 1. Department of Oncology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
    2. Department of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
  • Received:2008-05-18 Online:2008-09-20 Published:2008-09-15

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Objective: To study the effects of Bushen Rougan Recipe (BSRGR), a compound traditional Chinese herbal medicine, on hepatic fibrosis in rats induced by dimethylnitrosamine (DMN), and to explore its preliminary mechanism.
Methods: A total of 40 male Wistar rats were randomly divided to normal control group (n=10), untreated group (n=15), and BSRGR group (n=15). Except for the rats in normal control group, hepatic fibrosis in rats was induced by peritoneal injection of DMN for 4 weeks. And the rats in the BSRGR group were also intragastrically administered BSRGR within the 4-week course. At the end of the 4-week course, rats were all sacrificed. The liver functions were determined by automatic biochemistry analyzer, including serum total bilirubin (Tbil), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin. Expressions of connective tissue growth factor (CTGF) and collagen type Ⅰ mRNA in liver tissue were detected by reverse transcription polymerase chain reaction.
Results: It was found that the serum Tbil level and the activities of AST, ALT were declined in the BSRGR group as compared with those in the untreated group (P<0.01). The serum albumin content in the BSRGR group was increased as compared with that in the untreated group (P<0.01). The expressions of collagen type Ⅰ and CTGF mRNAs in the untreated group were higher than those in the BSRGR group (P<0.01).
Conclusion: BSRGR can decrease the expressions of collagen type Ⅰ and CTGF mRNAs in the rats with hepatic fibrosis, which may be one of possible mechanisms in treating hepatic fibrosis.

Key words: Bushen Rougan Recipe, hepatic fibrosis, dimethylnitrosamine, collagen type I, connective tissue growth factor, rats

CLC Number: 

  • R285.5

Table 1

Serum ALT, AST activities and albumin, Tbil levels in different groups（x±s）"

Group n Tbil (μmol/L) ALT (U/L) AST (U/L) Albumin (g/L)
Normal control 10 1.49±0.15 34.10±5.65 125.70±13.91 38.75±5.08
Untreated 13 35.46±4.59** 94.46±10.71** 284.38±38.86** 25.42±2.52**
BSRGR 14 22.14±5.71△△ 74.92±7.71△△ 224.01±13.19△△ 28.64±3.78△△

Figure 1

Expression of CTGF mRNA in liver tissue of rats in different groups Lane 1-3: Amplified products of β-actin (300 bp); Lane 4-6: Amplified products of CTGF mRNA in normal control group, untreated group and BSRGR group, respectively (383 bp)."

Figure 2

Expression of collagen type Ⅰ mRNA in liver tissue of rats in different groups Lane 1-3: Amplified products of β-actin (300 bp); Lane 4-6: Amplified products of collagen type Ⅰ mRNA in normal control group, untreated group and BSRGR group, respectively (225 bp)."

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