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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (11): 1159-1163.doi: 10.3736/jcim20081110

• Original Experimental Research • Previous Articles     Next Articles

Protective effect of Huangban Granule against light-induced retinal damage in rats

Yuan-yuan Gong, Yi Song, Zheng-gao Xie, Xing-wei Wu()   

  1. Department of Ophthalmology, First People’s Hospital,Shanghai Jiaotong University, Shanghai 200080, China
  • Received:2008-03-19 Online:2008-11-20 Published:2008-11-15

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Objective:

To observe the protective effect of Huangban Granule, a compound of traditional Chinese herbal medicine, on rats with retinal damage induced by light.


Methods:

A total of 24 male Sprague-Dawley (SD) rats were randomly divided into normal control group, untreated group and Huangban Granule group. Retinal light damage was induced by exposure to constant white fluorescent light for 5 hours at an illumination of 2 800 Lux. The Huangban Granule was given 10 days before light exposure until the animals were sacrificed in Huangban Granule group, and an equal volume of distilled water for the rats in untreated group. Electroretinogram (ERG) was recorded in all animals 2 weeks after light exposure and the animals were sacrificed for histopathological examination of retina. The outer nuclear layers (ONLs) on the superior and inferior retina were counted.


Results:

Fourteen days after light exposure, the ONLs on the superior retina were 3 to 6 in the untreated group and 7 to 9 in treatment group. There were 9 to 11 layers in normal group. The mean number of ONLs in the untreated group (4.68±1.64) was less than that in the treatment group (8.23±1.35) (P<0.01). B-wave amplitudes were (319.38±71.96) μV and (135.16±42.30) μV in Huangban Granule group and the untreated group respectively (P<0.01). A-wave amplitudes were (184.63±47.23) μV and (83.35±27.75) μV (P<0.01), and oscillatory potential amplitudes were (239.38±20.19) μV and (125.44±26.23) μV (P<0.01) respectively in the two group. There was no significant difference in implicit times of a-wave and b-wave among the three groups.


Conclusion:

Huangban Granule obviously protects both function and morphology of the retina from light-induced retinal damage in rats.

Key words: Huangban granule, electroretinography, light damage, retinal degeneration, rats

CLC Number: 

  • R774.13

Figure 1

Morphological changes 14 days after light damage in rats (HE staining, ×400) PE: Pigment epithelium; ONL: Outer nuclear layer; INL: Inner nuclear layer; GC: Ganglion cell layer. A: Normal control group, the layers of ONL were 9 to 11 and the neuclei arrangement was regular; B: Huangban Granule group, the layers of ONL were 7 to 9 and also in regular arrangement; C: Untreated group, the layers of ONL were 3 to 6 and in irregular arrangement; the outer and inner segments of photoreceptor were disappeared."

Figure 2

Changes of ONL thickness (layer number of neuclei) on different location across the vertical meridian 14 days after light damage in rats ONH refers to optic nerve head."

Table 1

Changes of ERG 14 days after light damage (x±s)"

Group n Implicit time (ms) Amplitude (μV)
a wave b wave a wave b wave OPs
Normal control 8 12.75±1.16 48.00±7.56 231.88±28.78 381.38±42.06 269.75±17.25
Untreated 8 11.88±0.83 55.25±7.42 83.35±27.75** 135.16±42.30** 125.44±26.23**
Huangban Granule 8 12.63±0.52 48.63±7.69 184.63±47.23*△△ 319.38±71.96*△△ 239.38±20.19*△△
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