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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (11): 1174-1177.doi: 10.3736/jcim20081113

• Original Experimental Research • Previous Articles     Next Articles

α-linolenic acid and linoleic acid in extract of Portulaca oleracea L. determined by high-performance liquid chromatography

Hai-liang Xin1,2, Yin-huan Hou3, Min Li4, Jin-cai Lu3, Chang-quan Lin2()   

  1. 1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
    2. Department of Drugs and Medical Appliances, No. 546 Hospital of People's Liberation Army, Urumqi, Xinjiang Uygur Autonomous Region 841700, China
    3. School of Traditiona Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province 110016, China
    4. Division of Military Health, Department of Naval Medicine, Second Military Medical University, Shanghai 200433, China
  • Received:2008-03-31 Online:2008-11-20 Published:2008-11-15

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Objective: 

To determine α-linolenic acid and linoleic acid in extract of Portulaca oleracea L. by high performance liquid chromatography (HPLC).


Methods: 

The determination was done with a Shim-pack CLC-ODS (250 mm×4.6 mm, 5 μm) and a DIKMA Easyguard C18 (10 mm×4.6 mm). Elution was employed with the mobile phase of methanol-acetonitrile-0.5% phosphonic acid (60︰22︰18) at flow rate of 1.1 ml/min. Column temperature was 26 ℃. Detection wavelength was 210 nm. Injection volume was 25 μl.


Results:

The standard curves of α-linolenic acid and linoleic acid were linear in the range 0.016 2 to 0.194 4 mg/ml and 0.016 9 to 0.203 0 mg/ml, respectively. The regression equations were A=2.915 8×10 7 C+12 250.9, r=0.999 9 and A=1.366 4×10 7 C–9 759.39, r=0.999 9, respectively. The average recovery rates were 100.5 % and 100.8 %, respectively.


Conclusion:

The present method (HPLC) may be considered to be reliable and simple for the determination of α-linolenic acid and linoleic acid in extract of Portulaca oleracea L.

Key words: Portulaca oleracea L, plant extracts, Chinese, alpha-linolenic acid, linoleic acid, chromatography, high performance liquid

CLC Number: 

  • R9-39

Table 1

Recovery rates of α-linolenic acid and linoleic acid"

Reference substance Assay (mg) Added
amount (mg)		 Detected
amount (mg)		 Recovery
rate (%)		 Average
recovery rate (%)		 RSD
(n=6, %)
α-linolenic acid 0.272 5
0.271 4
0.271 4
0.273 6
0.272 5
0.273 6		 0.243 0
0.243 0
0.243 0
0.243 0
0.243 0
0.243 0		 0.514 5
0.519 3
0.513 1
0.516 0
0.519 2
0.518 1		 99.59
101.99
99.48
99.78
101.54
100.62		 100.5 1.06
Linoleic acid 0.237 0
0.236 0
0.236 0
0.237 9
0.237 0		 0.253 8
0.253 8
0.253 8
0.253 8
0.253 8		 0.494 3
0.492 0
0.494 0
0.492 4
0.492 5		 101.38
100.85
101.63
100.26
100.67		 100.8 0.66

Table 2

Determination of α-linolenic acid and linoleic acid in extract of Portulaca oleracea L."

No α-linolenic acid Linoleic acid
Concentration (%) x (n=3, %) RSD (n=3, %) Concentration (%) x (n=3, %) RSD (n=3, %)
1 0.98 0.87
2 0.98 0.98 0.64 0.88 0.87 1.26
3 0.97 0.86

Figure 1

HPLC chromatograms of reference substance and sample A: Reference substance; B: Sample; Ⅰ: α-linolenic acid; Ⅱ: Linoleic acid"

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