Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (1): 105-108.doi: 10.3736/jcim20110117

• Short Report • Previous Articles     Next Articles

Identification and quantification of adrenaline from the leaves of Clerodendrum phlomidis using thin-layer chromatography

Muthu Kumaradoss Mohan Maruga Raja(), Ruchi Sureshbhai Patel, Shri Hari Mishra   

  1. Herbal Drug Technology Laboratory, Pharmacy Department, The Maharaja Sayajirao University of Baroda, Vadodara 390002, Gujarat, India
  • Received:2010-09-21 Accepted:2010-10-25 Online:2011-01-20 Published:2011-01-15

Key words: Clerodendrum, epinephrine, chromatography, thin-layer, plant extracts

[1] Mohan Maruga Raja MK, Mishra SH . Comprehensive review of Clerodendrum phlomidis: a traditionally used bitter[J]. J Chin Integr Med, 2010,8(6):510-524
Mohan Maruga Raja MK, Mishra SH. 传统苦味药用植物苦郎树[J]. 中西医结合学报, 2010,8(6):510-524
[2] Tretyn A, Kendrick RE . Acetylcholine in plants: presence, metabolism and mechanism of action[J]. Bot Rev, 1991,57(1):33-73
doi: 10.1007/BF02858764
[3] Kuklin AI, Conger BV . Catecholamines in plants[J]. J Plant Growth Regul, 1995,14(2):91-97
doi: 10.1007/BF00203119
[4] Smith TA . Phenethylamine and related compounds in plants[J]. Phytochemistry, 1977,16(1):9-18
doi: 10.1016/0031-9422(77)83004-5
[5] Lundstrom J. Simple isoquinoline alkaloids. In: Brossi A. The alkaloids: chemistry and biology. Vol 2 1. New York: Academic Press. 1983: 255-327
[6] Dai YR, Michaels PJ, Flores HE . Stimulation of ethylene production by catecholamines and phenylethylamine in potato cell suspension cultures[J]. Plant Growth Regul, 1993,12(3):219-222
doi: 10.1007/BF00027201
[7] Protacio CM, Dai YR, Lewis EF, Flores HE . Growth stimulation by catecholamines in plant tissue/organ cultures[J]. Plant Physiol, 1992,98(1):89-96
doi: 10.1104/pp.98.1.89
[8] Grossi G, Bargossi AM, Lucarelli C, Paradisi R, Sprovieri C, Sprovieri G . Improvements in automated analysis of catecholamine and related metabolites in biological samples by column-switching high-performance liquid chromatography[J]. J Chromatogr, 1991,541(1-2):273-284
doi: 10.1016/S0021-9673(01)95999-0
[9] Sarzanini C, Mentasti E, Nerva M . Determination of catecholamines by ion chromatography and electrochemical detection[J]. J Chromatogr A, 1994,671(1-2):259-264
doi: 10.1016/0021-9673(94)80248-3
[10] Hansen AM, Kristiansen J, Nielsen JL, Byrialsen K, Christensen JM . Validation of a high performance liquid chromatography analysis for the determination of noradrenaline and adrenaline in human urine with an on-line sample purification[J]. Talanta, 1999,50(2):367-379
doi: 10.1016/S0039-9140(99)00029-6
[11] Geissler HE, Mutschler E . Die direkte quantitative auswertung von dünnschichtchromatogrammen durch remissions- und fluoreszenzmessungen: 6. Mitteilung bestimmung von adrenalin, noradrenalin und dopamin aus dem harn[J]. J Chromatogr A, 1971,56:271-279
doi: 10.1016/S0021-9673(00)97809-9
[12] ICHHT Guideline. Validation of analytical procedures: text and methodology Q2(R1).
[1] Wan-jun Guo, Yi Wang, Yu Deng, Lin-yan Cheng, Xin Liu, Ruo-fan Xi, Sheng-jie Zhu, Xin-yi Feng, Liang Hua, Kan Ze, Jian-yong Zhu, Dong-jie Guo, Fu-lun Li. Therapeutic effects of the extract of Sancao Formula, a Chinese herbal compound, on imiquimod-induced psoriasis via cysteine-rich protein 61. Journal of Integrative Medicine, 2022, 20(4): 376-384.
[2] Abdulhakim Abubakar, Abdullahi Balarabe Nazifi, Idris Mohammed Maje, Yusuf Tanko, Joseph Akpojo Anuka, Ezzeldin Mukthar Abdurahman. Antihyperglycaemic activity of ethylacetate extract of Chlorophytum alismifolium in type 2 diabetes: The involvement of peroxisome proliferator-activated receptor-γ and dipeptidyl peptidase-4. Journal of Integrative Medicine, 2021, 19(1): 78-84.
[3] Rabie Kachkoul, Tarik Squalli Housseini, Mohamed Mohim, RadouaneEl Habbani, Youssef Miyah, Anissa Lahrichi. Chemical compounds as well as antioxidant and litholytic activities of Arbutus unedo L. leaves against calcium oxalate stones. Journal of Integrative Medicine, 2019, 17(6): 430-437.
[4] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[5] Asmae Assaidi, Ikram Dib, Monique Tits, Luc Angenot, Said Bellahcen, Nourelhouda Bouanani, Abdelkhaleq Legssyer, Mohammed Aziz, Hassane Mekhfi, Mohammed Bnouham, Michel Frederich, Abderrahim Ziyyat. Chenopodium ambrosioides induces an endothelium-dependent relaxation of rat isolated aorta. Journal of Integrative Medicine, 2019, 17(2): 115-124.
[6] Rohitash Jamwal. Bioavailable curcumin formulations: A review of pharmacokinetic studies in healthy volunteers. Journal of Integrative Medicine, 2018, 16(6): 367-374.
[7] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Thongchai Koobkokkruad, Chutichot Pattamadilok, Nontima Vardhanabhuti . Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2018, 16(6): 427-434.
[8] Shohini Chakraborty, Nashra Afaq, Neelam Singh, Sukanta Majumdar. Antimicrobial activity of Cannabis sativaThuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus. Journal of Integrative Medicine, 2018, 16(5): 350-357.
[9] Sandeep Kumar Singh, Arjun Patra. Evaluation of phenolic composition, antioxidant, anti-inflammatory and anticancer activities of Polygonatum verticillatum (L.). Journal of Integrative Medicine, 2018, 16(4): 273-282.
[10] Bruno José Martins Da Silva, Amanda Anastácia Pinto Hage, Edilene Oliveira Silva, Ana Paula Drummond Rodrigues. Medicinal plants from the Brazilian Amazonian region and their antileishmanial activity: A review. Journal of Integrative Medicine, 2018, 16(4): 211-222.
[11] Nanaware Sadhana, Sathiyanarayanan Lohidasan, Kakasaheb Ramoo Mahadik. Marker-based standardization and investigation of nutraceutical potential of Indian propolis. Journal of Integrative Medicine, 2017, 15(6): 483-494.
[12] Bhanuz Dechayont, Arunporn Itharat, Pathompong Phuaklee, Jitpisute Chunthorng-Orn, Thana Juckmeta, Nuntika Prommee, Nitra Nuengchamnong, Pintusorn Hansakul. Antioxidant activities and phytochemical constituents of Antidesma thwaitesianum Müll. Arg. leaf extracts. Journal of Integrative Medicine, 2017, 15(4): 310-319.
[13] Madan Mohan Pandey, Sayyada Khatoon, Subha Rastogi, Ajay Kumar Singh Rawat. Determination of flavonoids, polyphenols and antioxidant activity of Tephrosia purpurea: A seasonal study. Journal of Integrative Medicine, 2016, 14(6): 447-455.
[14] Nisha Panth, Keshav Raj Paudel, Rajendra Karki. Phytochemical profile and biological activity of Juglans regia. Journal of Integrative Medicine, 2016, 14(5): 359-373.
[15] Musa Toyin Yakubu, Abdulquadri Funmbi Olutoye. Aphrodisiac activity of aqueous extract of Anthonotha macrophylla P. Beauv. leaves in female Wistar rats. Journal of Integrative Medicine, 2016, 14(5): 400-408.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228