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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (9): 868-872.doi: 10.3736/jcim20090913

• Original Experimental Research • Previous Articles     Next Articles

Effects of ranitidine on pharmacokinetics of rhein from Dachengqi Decoction in rats after oral administration

Yan-yi Ren, Han-lin Gong, Wen-Fu Tang), Mei-hua Wan, Xi Huang   

  1. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2009-04-02 Accepted:2009-07-17 Online:2009-09-20 Published:2009-09-15
  • Contact: Wen-Fu Tang E-mail:wftang900@126.com

Objective

To explore the effects of ranitidine on pharmacokinetics of rhein in rats after oral administration of Dachengqi Decoction (DCQD), a compound traditional Chinese herbal medicine.
Methods

Twelve male Sprague-Dawley rats were divided into DCQD group and DCQD plus ranitidine group, and were orally administered with DCQD at a dose of 10 g/kg or DCQD (10 g/kg) combined with ranitidine (150 mg/kg), respectively. Blood samples were gathered after a series of time intervals. Metabolism of rhein was determined with a reversed-phase high-performance liquid chromatography with internal standard of 1, 8-dihydroxyanthraquinone and the data were analyzed with DAS 2.1 program. The pharmacokinetic parameters were compared between the two groups.
Results

The pharmacokinetic parameters of rhein in the DCQD group, including peak concentration (Cmax), area under the plasma concentration-time curve (AUC), distribution phase half-life (t1/2α), elimination rate constant (K10) and central to peripheral transfer rate constant (K12), were significantly different to those in the DCQD plus ranitidine group (P<0.05, P<0.01). There were no significant differences in the other parameters between the two groups.
Conclusion

Ranitidine can influence the pharmacokinetics of rhein in rats after oral administration of DCQD.

Key words: Dachengqi Decoction, Ranitidine, Rhein, Chromatography, High pressure liquid, Pharmacokinetics, Rats

Figure 1

Chemical structure of rhein"

Figure 2

Typical chromatograms for determination of rhein and 1, 8-dihydroxyanthraquinone in plasma A: Chromatogram of a blank plasma sample; B: Chromatogram of a plasma sample spiked with rhein (peak 1) and internal standard of 1, 8-dihydroxyanthraquinone (peak 2); C: Chromatogram of a plasma sample collected 20 min after oral administration of DCQD and ranitidine; D: Chromatogram of a plasma sample collected 20 min after oral administration of DCQD alone."

Table 1

Intra-day and inter-day accuracy and precision for rhein"

n Concentration (μg/mL) Concentration measured
($\bar{x}$±s, μg/mL)
Accuracy (%) Average (%) RSD (%) Average (%)
Intra-day 6 0.769 1 0.711 5±0.049 2 92.51 94.09 6.91 5.33
6 3.076 2 2.847 4±0.134 5 92.56 94.09 4.72 5.33
6 15.381 0 14.948 0±0.651 3 97.18 94.09 4.36 5.33
Inter-day 6 0.769 1 0.712 6±0.052 5 92.65 94.42 4.03 5.42
6 3.076 2 2.910 7±0.141 9 94.62 94.42 4.88 5.42
6 15.381 0 14.765 0±0.595 1 96.00 94.42 7.37 5.42

Table 2

Recovery of rhein in rat plasma"

Concentration spiked (μg/mL) n Recovery (Mean, %) Average (%) RSD (%) Average (%)
0.769 1 6 92.66 94.42 7.4 5.4
3.076 2 6 94.62 94.42 4.8 5.4
15.381 6 95.99 94.42 4.0 5.4

Figure 3

Concentration-time curve of rhein in rats Data points represent the mean (n=6)."

Table 3

Pharmacokinetic parameters of rhein in rat plasma"

Parameter ($\bar{x}$±s) DCQD (n=6) DCQD plus ranitidine (n=6) P value
t1/2α (h) 0.49±0.10 0.36±0.05 0.017 7
K10 (/h) 1.60±0.42 1.06±0.29 0.027 1
K12 (/h) 1.28±0.46 2.99±0.79 0.001 0
AUC(0-t) (mg/L·h) 12.25±3.76 6.28±2.27 0.007 7
AUMC(0-t) (mg/L·h2) 44.13±11.52 25.21±9.71 0.011 7
Cmax (mg/L) 6.01±3.59 2.25±1.12 0.034 5
[1] Katakai M, Akamaru T, Tani T . An analysis of the frequency of formulations and crude drugs described in Shan-Han-Lun[J]. Yakushigaku Zasshi, 2002,37(1):28-35
pmid: 12408167
[2] Xia Q, Huang ZW, Jiang JM, Chen GY, Yang XN, Tang WF, Zhang ZD, Liu XB, Hu WM, Tian BL, Li CS, Yan LN, Luo CX, Kang Y, Yuan CX, Song B, Luo Y . "Yi-Huo-Qing-Xia" method as the main therapy in integrated traditional Chinese and Western medicine on severe acute pancreatitis: a report of 1 161 cases[J]. Zhongguo Zhong Xi Yi Jie He Ji Jiu Za Zhi, 2006,13(3):131-134
夏庆, 黄宗文, 蒋俊明, 陈光远, 杨晓楠, 唐文富, 张肇达, 刘续宝, 胡伟明, 田伯乐, 李全生, 严律南, 罗传兴, 康焰, 袁朝新, 宋彬, 罗燕 . 以"益活清下"为主的中西医结合综合疗法治疗重症急性胰腺炎1 161例疗效报告[J]. 中国中西医结合急救杂志, 2006,13(3):131-134
[3] Tang WF, Wan MH, Zhu L, Chen GY, Xia Q, Huang X . Immuno-modulatory effect of somatostatin combined with traditional Chinese medicine on severe acute pancreatitis at early stage: a randomized control trial[J]. J Chin Integr Med, 2005,3(2):103-107
唐文富, 万美华, 朱林, 陈光远, 夏庆, 黄熙 . 生长抑素联合中药对急性胰腺炎患者免疫功能的影响及临床疗效[J]. 中西医结合学报, 2005,3(2):103-107
[4] Tang WF, Wan MH, Huang QR, Zhu ZY, Zhao JL, Wu YZ, Huang X . Effect of Da-Cheng-Qi decoction on the pharmacokinetics of ranitidine in rats[J]. Biomed Chromatogr, 2008,22(8):851-856
doi: 10.1002/bmc.1002 pmid: 18384065
[5] Tang WF, Yu Q, Wan MH, Qin F, Wang YG, Chen GY, Liang MZ, Huang X . Simultaneous determination and pharmacokinetic studies of aloe emodin and chrysophanol in rats after oral administration of Da-Cheng-Qi decoction by high-performance liquid chromatography[J]. Biomed Chromatogr, 2007,21(7):701-707
doi: 10.1002/(ISSN)1099-0801
[6] Tang WF, Huang X, Yu Q, Qin F, Wan MH, Wang YG, Liang MZ . Determination and pharmacokinetic comparison of rhein in rats after oral dosed with Da-Cheng-Qi decoction and Xiao-Cheng-Qi decoction[J]. Biomed Chromatogr, 2007,21(11):1186-1190
doi: 10.1002/(ISSN)1099-0801
[7] Tang WF, Wan M, Zhu Z, Chen G, Huang X . Simultaneous determination of eight major bioactive compounds in Dachengqi Tang(DT) by high-performance liquid chromatography[J]. Chin Med, 2008,( 3):5
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