[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (5): 441-446.doi: 10.3736/jcim20090508

• Original Experimental Research • Previous Articles     Next Articles

Effects of Niupo Zhibao Pellet on high-mobility group box-1 protein expression in lung tissues of endotoxin shock rats

Hui Lia,Shai-hui Dub,Sai-xia Zhanga,Ru-dong Denga,Chun Lia,Yi-wei Lia,Dong-feng Chena,Jian-hong Zhou a   

  1. a Department of Anatomy, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Proyince, China
    b Department of Internal Medicine, Shenzhen Hospital of Chinese Medicine, Shenzhen 518033; Guangdong Province, China
  • Received:2008-10-23 Accepted:2009-03-02 Online:2009-05-20 Published:2009-05-15

Objective

To observe the effects of Niupo Zhibao Pellet, a compound traditional Chinese herbal medicine, on high-mobility group box-1 protein (HMGB1) expression in lung tissues of rats with endotoxin shock.
Methods

Thirty SPF Sprague-Dawley rats were randomly divided into control group, lipopolysaccharide (LPS) group and Niupo Zhibao Pellet group. Rats in Niupo Zhibao Pellet group were consecutively administered 7 days with 3 mL (1 g/L) Niupo Zhibao Pellet saline suspension every day by intragastric administration. Endotoxin shock was induced in rats of the LPS and Niupo Zhibao Pellet groups by intravenous injection of LPS (1.5 mg/kg) and intraperitoneal injection of D-galactosamine (100 mg/kg). Expression of HMGB1 in lung tissues was measured by immunohistochemical method with diaminobenzidine (DAB) coloration, fluorescein isothiocyanate (FITC) labeling, and by Western blotting.
Results

Expression of HMGB1 in lung tissues in the LPS group was increased and that in Niupo Zhibao Pellet group was higher than that in the LPS group and the control group. HMGB1 was presented in the cytoplasm of positive cells in the LPS group, but in the nucleus of positive cells in the Niupo Zhibao Pellet group. However, HMGB1 was little expressed in the lung tissues of normal rats.
Conclusion

Niupo Zhibao Pellet can increase HMGB1 expression and locate HMGB1 in the nucleus but not the cytoplasm, which may be one of its mechanisms in reducing endotoxin shock.

Key words: Niupo Zhibao Pellet, Lung injury, Endotoxin shock, High-mobility group box-1 protein, Rats

Figure 1

Expression of HMGB1 in lung tissues of endotoxin shock rats observed by immunohistochemical method with DAB coloration In A and D of the control group, arrows show the same pulmonary macrophage with HMGB1 in the cytoplasm; in B of the LPS group, arrows show the HMGB1 positive cells in alveolar septum, and in E of the LPS group, arrows show the HMGB1 positive lymphocytes and macrophages in alveolar space; in C and F of the Niupo Zhibao Pellet group, arrows show HMGB1 positive cells in alveolar septum."

Figure 2

Expression of HMGB1 in lung tissues of endotoxin shock rats observed by laser-confocal microscope FITC-stained HMGB1 was green and PI-stained nucleus was red. Yellow in merged image indicates overlap of red and green labels."

Figure 3

Fluorescent quantitative analysis of HMGB1 expression observed by laser-confocal microscope Data were represented as x±s, n=10. *P<0.05, **P<0.01, vs control group; △P<0.05, vs LPS group."

Figure 4

HMGB1 expression observed by Western blotting 1: Control group; 2: LPS group; 3: Niupo Zhibao Pellet group."

[1] Wang H, Bloom O, Zhang M, Vishnubhakat JM, Ombrellino M, Che J, Frazier A, Yang H, Ivanova S, Borovikova L, Manogue KR, Faist E, Abraham E, Andersson J, Andersson U, Molina PE, Abumrad NN, Sama A, Tracey KJ . HMG-1 as a late mediator of endotoxin lethality in mice[J]. Science, 1999,285(5425):248-251
doi: 10.1126/science.285.5425.248
[2] Li J, Wang H, Mason JM, Levine J, Yu M, Ulloa L, Czura CJ, Tracey KJ, Yang H . Recombinant HMGB1 with cytokine-stimulating activity[J]. J Immunol Methods, 2004,289(1-2):211-223
doi: 10.1016/j.jim.2004.04.019
[3] Yang H, Ochani M, Li J, Qiang X, Tanovic M, Harris HE, Susarla SM, Ulloa L, Wang H, DiRaimo R, Czura CJ, Wang H, Roth J, Warren HS, Fink MP, Fenton MJ, Andersson U, Tracey KJ .Reversing established sepsis with antagonists of endogenous high-mobility group box 1[J]. Proc Natl Acad Sci U S A, 2004,101(1):296-301
doi: 10.1073/pnas.2434651100
[4] Zhang WH, Wang GH, Zhang JJ . Research on production of Niupo Zhibao Pellet[J]. Zhong Cheng Yao, 1999,21(3):112-114
张文惠, 汪国华, 张佳佳 . 牛珀至宝微丸的研制[J]. 中成药, 1999,21(3):112-114
[5] Luo ZY . Empirical study on septic shock with the method of dredging obstruction and preventing exhaustion[J]. Zhongguo Bing Li Sheng Li Za Zhi, 1994,10(S):67-72
罗正曜 . 开闭固脱法治疗感染性休克的实验研究[J]. 中国病理生理杂志, 1994,10(S):67-72
[6] Shen YP, Deng RD, Li H, Du SH, Chen DF, Zhou JH, Li YW . Optimized endotoxin shock model sensitized with D-galactosamine in Sprague-Dawley rat[J]. Zhongguo Bing Li Sheng Li Za Zhi, 2007,23(8):1662-1664
doi: 1000-4718
沈逸萍, 邓汝东, 黎晖, 杜少辉, 陈东风, 周健洪, 李伊为 . D-氨基半乳糖致敏的内毒素休克SD大鼠模型[J]. 中国病理生理杂志, 2007,23(8):1662-1664
doi: 1000-4718
[7] Wang H, Li W, Li J, Rendon-Mitchell B, Ochani M, Ashok M, Yang L, Yang H, Tracey KJ, Wang P, Sama AE . The aqueous extract of a popular herbal nutrient supplement, Angelica sinensis, protects mice against lethal endotoxemia and sepsis[J]. J Nutr, 2006,136(2):360-365
doi: 10.1093/jn/136.2.360
[8] Xu J, Liu ZF, Wang J, Deng P, Jiang Y . Study of localization and translocation of human high mobility group protein B1 in eukaryotic cells[J]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue, 2006,18(6):338-342
徐佳, 刘志锋, 王娟, 邓鹏, 姜勇 . 人高迁移率族蛋白B1细胞内定位及移位研究[J]. 中国危重病急救医学, 2006,18(6):338-342
[9] Bonaldi T, Talamo F, Scaffidi P, Ferrera D, Porto A, Bachi A, Rubartelli A, Agresti A, Bianchi ME . Monocytic cells hyperacetylate chromatin protein HMGB1 to redirect it towards secretion[J]. EMBO J, 2003,22(20):5551-5560
doi: 10.1093/emboj/cdg516
[10] Ulloa L, Messmer D . High-mobility group box 1(HMGB1) protein: friend and foe[J]. Cytokine Growth Factor Rev, 2006,17(3):189-201
doi: 10.1016/j.cytogfr.2006.01.003
[11] Lotze MT, Tracey KJ . High-mobility group box 1 protein(HMGB1): nuclear weapon in the immune arsenal[J]. Nat Rev Immunol, 2005,5(4):331-342
doi: 10.1038/nri1594
[12] Kokkola R, Andersson A, Mullins G, Ostberg T, Treutiger CJ, Arnold B, Nawroth P, Andersson U, Harris RA, Harris HE . RAGE is the major receptor for the proinflammatory activity of HMGB1 in rodent macrophages[J]. Scand J Immunol, 2005,61(1):1-9
doi: 10.1111/sji.2005.61.issue-1
[13] Yu M, Wang H, Ding A, Golenbock DT, Latz E, Czura CJ, Fenton MJ, Tracey KJ, Yang H . HMGB1 signals through toll-like receptor(TLR) 4 and TLR2[J]. Shock, 2006,26(2):174-179
doi: 10.1097/01.shk.0000225404.51320.82
[14] Li J, Kokkola R, Tabibzadeh S, Yang R, Ochani M, Qiang X, Harris HE, Czura CJ, Wang H, Ulloa L, Wang H, Warren HS, Moldawer LL, Fink MP, Andersson U, Tracey KJ, Yang H . Structural basis for the proinflammatory cytokine activity of high mobility group box 1[J]. Mol Med, 2003,9(1-2):37-45
doi: 10.1007/BF03402105
[15] Park JS, Arcaroli J, Yum HK, Yang H, Wang H, Yang KY, Choe KH, Strassheim D, Pitts TM, Tracey KJ, Abraham E . Activation of gene expression in human neutrophils by high mobility group box 1 protein[J]. Am J Physiol Cell Physiol, 2003,284(4):C870-C879
doi: 10.1152/ajpcell.00322.2002
[1] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[2] Lawrence A. Olatunji, Taofeek O. Usman, Joseph O. Adebayo, Victoria A. Olatunji . Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats. Journal of Chinese Integrative Medicine, 2012, 10(9): 1049-1055.
[3] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[4] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[5] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[6] Karuna A. Talpate, Uma A. Bhosale, Mandar R. Zambare. Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 939-947.
[7] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[8] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[9] Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[10] Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[11] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[12] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[13] Feng Xin-ying, Zhu Min, Zhang Qi-qi, Chen Yi-ping, Li Wen-wei. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. Journal of Chinese Integrative Medicine, 2012, 10(7): 777-783.
[14] Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
[15] Hui-qing Zhang , Zi-fei Yin , Jia-yu Sheng , Zhu-qian Jiang , Bao-yu Wu , Yong-hua Su. A comparison study of pharmacokinetics between bufalin-loaded bovine serum albumin nanoparticles and bufalin in rats. Journal of Chinese Integrative Medicine, 2012, 10(6): 674-680.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[an error occurred while processing this directive]