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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (5): 441-446.doi: 10.3736/jcim20090508

• Original Experimental Research • Previous Articles     Next Articles

Effects of Niupo Zhibao Pellet on high-mobility group box-1 protein expression in lung tissues of endotoxin shock rats

Hui Lia,Shai-hui Dub,Sai-xia Zhanga,Ru-dong Denga,Chun Lia,Yi-wei Lia,Dong-feng Chena,Jian-hong Zhou a   

  1. a Department of Anatomy, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Proyince, China
    b Department of Internal Medicine, Shenzhen Hospital of Chinese Medicine, Shenzhen 518033; Guangdong Province, China
  • Received:2008-10-23 Accepted:2009-03-02 Online:2009-05-20 Published:2009-05-15

Objective

To observe the effects of Niupo Zhibao Pellet, a compound traditional Chinese herbal medicine, on high-mobility group box-1 protein (HMGB1) expression in lung tissues of rats with endotoxin shock.
Methods

Thirty SPF Sprague-Dawley rats were randomly divided into control group, lipopolysaccharide (LPS) group and Niupo Zhibao Pellet group. Rats in Niupo Zhibao Pellet group were consecutively administered 7 days with 3 mL (1 g/L) Niupo Zhibao Pellet saline suspension every day by intragastric administration. Endotoxin shock was induced in rats of the LPS and Niupo Zhibao Pellet groups by intravenous injection of LPS (1.5 mg/kg) and intraperitoneal injection of D-galactosamine (100 mg/kg). Expression of HMGB1 in lung tissues was measured by immunohistochemical method with diaminobenzidine (DAB) coloration, fluorescein isothiocyanate (FITC) labeling, and by Western blotting.
Results

Expression of HMGB1 in lung tissues in the LPS group was increased and that in Niupo Zhibao Pellet group was higher than that in the LPS group and the control group. HMGB1 was presented in the cytoplasm of positive cells in the LPS group, but in the nucleus of positive cells in the Niupo Zhibao Pellet group. However, HMGB1 was little expressed in the lung tissues of normal rats.
Conclusion

Niupo Zhibao Pellet can increase HMGB1 expression and locate HMGB1 in the nucleus but not the cytoplasm, which may be one of its mechanisms in reducing endotoxin shock.

Key words: Niupo Zhibao Pellet, Lung injury, Endotoxin shock, High-mobility group box-1 protein, Rats

Figure 1

Expression of HMGB1 in lung tissues of endotoxin shock rats observed by immunohistochemical method with DAB coloration In A and D of the control group, arrows show the same pulmonary macrophage with HMGB1 in the cytoplasm; in B of the LPS group, arrows show the HMGB1 positive cells in alveolar septum, and in E of the LPS group, arrows show the HMGB1 positive lymphocytes and macrophages in alveolar space; in C and F of the Niupo Zhibao Pellet group, arrows show HMGB1 positive cells in alveolar septum."

Figure 2

Expression of HMGB1 in lung tissues of endotoxin shock rats observed by laser-confocal microscope FITC-stained HMGB1 was green and PI-stained nucleus was red. Yellow in merged image indicates overlap of red and green labels."

Figure 3

Fluorescent quantitative analysis of HMGB1 expression observed by laser-confocal microscope Data were represented as x±s, n=10. *P<0.05, **P<0.01, vs control group; △P<0.05, vs LPS group."

Figure 4

HMGB1 expression observed by Western blotting 1: Control group; 2: LPS group; 3: Niupo Zhibao Pellet group."

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