牛珀至宝微丸对内毒素休克大鼠肺组织高迁移率族蛋白B1表达的影响

doi:10.3736/jcim20090508

Abstract

Objective

To observe the effects of Niupo Zhibao Pellet, a compound traditional Chinese herbal medicine, on high-mobility group box-1 protein (HMGB1) expression in lung tissues of rats with endotoxin shock.
Methods

Thirty SPF Sprague-Dawley rats were randomly divided into control group, lipopolysaccharide (LPS) group and Niupo Zhibao Pellet group. Rats in Niupo Zhibao Pellet group were consecutively administered 7 days with 3 mL (1 g/L) Niupo Zhibao Pellet saline suspension every day by intragastric administration. Endotoxin shock was induced in rats of the LPS and Niupo Zhibao Pellet groups by intravenous injection of LPS (1.5 mg/kg) and intraperitoneal injection of D-galactosamine (100 mg/kg). Expression of HMGB1 in lung tissues was measured by immunohistochemical method with diaminobenzidine (DAB) coloration, fluorescein isothiocyanate (FITC) labeling, and by Western blotting.
Results

Expression of HMGB1 in lung tissues in the LPS group was increased and that in Niupo Zhibao Pellet group was higher than that in the LPS group and the control group. HMGB1 was presented in the cytoplasm of positive cells in the LPS group, but in the nucleus of positive cells in the Niupo Zhibao Pellet group. However, HMGB1 was little expressed in the lung tissues of normal rats.
Conclusion

Niupo Zhibao Pellet can increase HMGB1 expression and locate HMGB1 in the nucleus but not the cytoplasm, which may be one of its mechanisms in reducing endotoxin shock.

Key words: Niupo Zhibao Pellet, Lung injury, Endotoxin shock, High-mobility group box-1 protein, Rats

Hui Li , Shai-hui Du, Sai-xia Zhang, Ru-dong Deng, Chun Li, Yi-wei Li, Dong-feng Chen, Jian-hong Zhou . Effects of Niupo Zhibao Pellet on high-mobility group box-1 protein expression in lung tissues of endotoxin shock rats. Journal of Chinese Integrative Medicine, 2009, 7(5): 441-446.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090508

http://www.jcimjournal.com/EN/Y2009/V7/I5/441

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Effects of Niupo Zhibao Pellet on high-mobility group box-1 protein expression in lung tissues of endotoxin shock rats

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