[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (10): 1171-1178.doi: 10.3736/jcim20121015

Previous Articles     Next Articles

Renoprotective effects of Moringa oleifera pods in 7,12-dimethylbenz[a]anthracene-exposed mice

Veena Sharma1, Ritu Paliwal1, Pracheta Janmeda1, Shatruhan Sharma2   

  • Received:2012-04-10 Accepted:2012-05-03 Online:2012-10-20 Published:2018-10-15

RichHTML

1

PDF (PC)

619

Like

Objective: To investigate the potential of hydroethanolic extract of Moringa oleifera (MOHE) against 7, 12-dimethylbenz[a]anthracene (DMBA)-induced toxicity in male Swiss albino mice. .

Methods: Experimental mice were respectively pretreated with 200 and 400 mg/kg of MOHE, and 0.5% and 1% of butylated hydroxyanisole (BHA) for two weeks prior to the administration of 15 mg/kg of DMBA, respectively. Levels of xenobiotic metabolizing enzymes such as cytochrome (Cyt) P450 and Cyt b5, activities of reduced glutathione (GSH) and glutathione-S-transferase (GST) and renal aspartate aminotransaminase (AST), alanine aminotransaminase (ALT) and alkaline phosphatase (ALP), and content of protein and total cholesterol were measured to determine the nephrotoxicity caused by DMBA and to elucidate the ameliorating role of M. oleifera.

Results: Single oral administration of 15 mg/kg of DMBA resulted in significant increases in Cyt P450 and Cyt b5 (P<0.01). The toxic effect of DMBA was justified by the significant decreases in the activities of GSH and GST in renal tissues (P<0.05). The levels of renal AST, ALT and ALP and protein content which are indicative of renocellular damage were also found decreased along with significant increase in total cholesterol content in DMBA-treated mice (P<0.01). The DMBA-induced alterations in the tissues were significantly reversed after pretreatment with 200 and 400 mg/kg of MOHE orally for 14 d (P<0.01).

Conclusion: The effects of MOHE in enhancing the levels of antioxidants and enhancing the levels of biochemical assays in DMBA-induced carcinogenesis are by reducing the formation of free radicals. This study rationalizes the ethnomedicinal use of M. oleifera for the protection against nephrotoxicity induced by chemical carcinogens.

Key words: Moringa oleifera, plant extracts, 9,10-dimethyl-1,2-benzanthracene, carcinogens, cytotoxicity, immunologic, mice

"

Group n Cyt P450 Cyt b5
Control 10 0.87±0.07 0.31±0.08
DMBA (15 mg/kg) 10 7.83±0.09** 4.95±0.02**
MOHE (200 mg/kg) plus DMBA (15 mg/kg) 10 5.92±0.05△△ 3.57±0.06△△
MOHE (400 mg/kg) plus DMBA (15 mg/kg) 10 2.46±0.08△△ 1.59±0.09△△
BHA (0.5%) plus DMBA
(15 mg/kg)		 10 6.25±0.15△△ 4.32±0.11△△
BHA (1%) plus DMBA
(15 mg/kg)		 10 5.32±0.12△△ 2.81±0.13△△

"

Group n GSH (nmol/g) GST (nmol CDNB/(min·mg protein))
Control 6 1.73±0.20 93.11±0.03
DMBA (15 mg/kg) 6 1.23±0.13** 61.23±0.09*
MOHE (200 mg/kg) plus DMBA (15 mg/kg) 6 1.64±0.15△△ 81.67±0.06△△
MOHE (400 mg/kg) plus DMBA (15 mg/kg) 6 1.75±0.05△△ 89.11±0.08△△
BHA (0.5%) plus DMBA (15 mg/kg) 6 1.55±0.07△△ 69.43±0.15△△
BHA (1%) plus DMBA (15 mg/kg) 6 1.59±0.05△△ 75.34±0.07△△

"

Group n AST (IU/L) ALT (IU/L) ALP (μmol PNP/
(min·g tissue))		 Total protein
(g/mL)		 Total cholesterol
(mg/g)
Control 10 75.23±0.03 47.34±0.02 95.23±0.05 5.32±0.06 75.34±0.04
DMBA (15 mg/kg) 10 36.55±0.07** 21.54±0.06** 52.33±0.12** 3.98±0.09** 116.28±0.08**
MOHE (200 mg/kg) plus
DMBA (15 mg/kg)		 10 61.43±0.05△△ 37.33±0.06△△ 81.44±0.13△△ 4.98±0.13△△ 92.87±0.05△△
MOHE (400 mg/kg) plus
DMBA (15 mg/kg)		 10 72.54±0.04△△ 44.67±0.07△△ 92.44±0.05△△ 5.25±0.07△△ 81.65±0.03△△
BHA (0.5%) plus DMBA
(15 mg/kg)		 10 43.58±0.17△△ 26.41±0.09△△ 63.77±0.09△△ 4.32±0.09△△ 109.65±0.10△△
BHA (1%) plus DMBA
(15 mg/kg)		 10 55.67±0.14△△ 32.42±0.02△△ 76.42±0.06△△ 4.56±0.02△△ 101.23±0.12△△
[1] Sharma P, Parmar J, Verma P, Sharma P, Goyal PK . Chemopreventive effect of Phyllanthus niruri on DMBA induced skin papillomagenesis in Swiss albino mice[J]. Int J Biol Med Res, 2010,1(4):158-164
[2] Moringa oleifera Lam . on hepatic carcinogen metabolising enzymes, antioxidant parameters and skin papillomagenesis in mice[J]. Asian Pac J Cancer Prev, 2003,4(2):131-139
[3] International Agency for Research on Cancer. IARC monographs on the evaluation of carcinogenic risk of chemicals to humans. Vol. 3. Certain polycyclic aromatic hydrocarbons and heterocyclic compounds. Lyon: International Agency for Research on Cancer. 1973.
doi: 10.1136/jcp.33.1.98-b pmid: 6998267
[4] Internaitonal Agency of Research on Cancer. IARC monographs on the evaluation of carcinogenic risk of chemicals to humans. Vol. 32. Polynuclear aromatic compounds, Part 1, chemical, environmental and experimental data. Lyon: International Agency for Research on Cancer. 1983.
doi: 10.1136/jcp.33.1.98-b pmid: 6998267
[5] International Agency for Research on Cancer. IARC monographs on the evaluation of carcinogenic risks to humans supplement. Overall evaluations of carcinogenicity: an updating of IARC monographs volumes 1 to 42. Lyon: International Agency for Research on Cancer. 1987.
[6] Miyata M, Furukawa M, Takahashi K, Gonzalez FJ, Yamazoe Y . Mechanism of 7,12-dimethylbenz[a]anthracene-induced immunotoxicity: role of metabolic activation at the target organ[J]. Jpn J Pharmacol, 2001,86(3):302-309
doi: 10.1254/jjp.86.302
[7] Lindhe O, Granberg L, Brandt I . Target cells for cytochrome p450-catalysed irreversible binding of 7,12-dimethylbenz[a]anthracene(DMBA) in rodent adrenal glands[J]. Arch Toxicol, 2002,76(8):460-466
doi: 10.1007/s00204-002-0367-1
[8] Buters J, Quintanilla-Martinez L, Schober W, Soballa VJ, Hintermair J, Wolff T, Gonzalez FJ, Greim H . CYP1B1 determines susceptibility to low doses of 7,12-dimethylbenz[a]anthracene-induced ovarian cancers in mice: correlation of CYP1B1-mediated DNA adducts with carcinogenicity[J]. Carcinogenesis, 2003,24(2):327-334
doi: 10.1093/carcin/24.2.327
[9] Paliwal R, Sharma V , Pracheta.A review on horse radish tree(Moringa oleifera): a multipurpose tree with high economic and commercial importance[J]. Asian J Biotechnol, 2011,3(4):317-328
doi: 10.3923/ajbkr.2011.317.328
[10] Moringa oleifera Lam . in amelioration of DMBA-induced renal carcinogenesis in Swiss albino mice[J]. Biol Med, 2011,3(2):27-35
[11] Paliwal R, Sharma V, , Pracheta Sharma SH . Hepatoprotective and antioxidant potential of Moringa oleifera pods against DMBA-induced hepatocarcinogenesis in male mice. Int[J] Drug Dev Res. 2011; 3(2):128-138.
[12] Paliwal R, Sharma V, , Pracheta Sharma S . Elucidation of free radical scavenging and antioxidant activity of aqueous and hydro-ethanolic extracts of Moringa oleifera pods. Res[J] Pharm Technol. 2011; 4(4):566-571.
[13] Moringa oleifera Lam . pods[J]. J Pharm Res, 2011,4(2):554-557
[14] Song LL, Myers SR, Lantvit D, Lubet RA, Steele VE, Kelloff GJ, Moon RC, Pezzuto JM . Chemoprevention of DMBA-induced mammary carcinogenesis: relationship between induction of phase Ⅱ enzymes, effects on DMBA-induced hemoglobin adducts and decreases in mammary tumor multiplicity[J]. Polycycl Aromat Compd, 2000,18(2):193-210
doi: 10.1080/10406630008028145
[15] Mohandas J, Marshall JJ, Duggin GG, Horvath JS, Tiller DJ . Differential distribution of glutathione and glutathione-related enzymes in rabbit kidney. Possible implications in analgesic nephropathy[J]. Biochem Pharmacol, 1984,33(11):1801-1807
doi: 10.1016/0006-2952(84)90353-8
[16] Omura T, Sato R . The carbon monoxide binding pigment of liver[J]. J Biol Chem, 1964, 2392370-2378
[17] Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR . Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite[J]. Pharmacology, 1974,11(3):151-169
doi: 10.1159/000136485
[18] Habig WH, Pabst MJ, Jakoby WB.GlutathioneS-transferases . The first enzymatic step in mercapturic acid formation[J]. J Biol Chem, 1974,249(22):7130-7139
[19] Reitman S, Frankel S . A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases[J]. Am J Clin Pathol, 1957,28(1):56-63
doi: 10.1093/ajcp/28.1.56
[20] Sadashivam S, Manickam A . Biochemical methods[M]. New Delhi: New Age International Pvt Ltd Publishers, 2004, 193-194
[21] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ . Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951,193(1):265-275
[22] Zak B . Cholesterol methodologies: a review[J]. Clin Chem, 1977,23(7):1201-1214
[23] Pezzuto JM . Plant-derived anticancer agents[J]. Biochem Pharmacol, 1997,53(2):121-133
doi: 10.1016/S0006-2952(96)00654-5
[24] Prior RL, Cao G . In vivo total antioxidant capacity: comparison of different analytical methods[J]. Free Radic Biol Med, 1999,27(11-12):1173-1181
doi: 10.1016/S0891-5849(99)00203-8 pmid: 10641708
[25] Izzotti A, Camoirano A, Cartiglia C, Grubbs CJ, Lubet RA, Kelloff GJ, De Flora S . Patterns of DNA adduct formation in liver and mammary epithelial cells of rats treated with 7,12-dimethylbenz(a)anthracene, and selective effects of chemopreventive agents[J]. Cancer Res, 1999,59(17):4285-4290
[26] Bharali R, Azad MR, Tabassum J . Chemopreventive action of Boerhaavia diffusa on DMBA-induced skin carcinogenesis in mice[J]. Indian J Physiol Pharmacol, 2003,47(4):459-464
pmid: 15266960
[27] Parimalakrishnan S, Dey A, Manavalan R . Effect of methanolic extract of Cleome chelidonii on drug metabolising enzymes, antioxidant status and chemomodulatory efficacy of mice[J]. J Basic Appl Sci, 2009,5(1):37-46
[28] Bianchini F, Vainio H . Allium vegetables and organosulfur compounds: do they help prevent cancer? Environ Health Perspect. 2001; 109(9):893-902.
doi: 10.2307/3454989
[29] Fahmy H, Zjawiony JK, Konoshima T, Tokuda H, Khan S, Khalifa S . Potent skin cancer chemopreventing activity of some novel semi-synthetic cembranoids from marine sources[J]. Mar Drugs, 2006,4(2):28-36
doi: 10.3390/md402028
[30] Liu J, Wang X, Shigenaga MK, Yeo HC, Mori A, Ames BN . Immobilization stress causes oxidative damage to lipid, protein, and DNA in the brain of rats[J]. FASEB J, 1996,10(13):1532-1538
doi: 10.1006/excr.1996.0334 pmid: 8940299
[31] Preston TJ, Abadi A, Wilson L, Singh G . Mitochondrial contributions to cancer cell physiology: potential for drug development[J]. Adv Drug Deliv Rev, 2001,49(1-2):45-61
doi: 10.1016/S0169-409X(01)00127-2 pmid: 11377802
[32] Parmar J, Sharma P, Verma P, Sharma P, Goyal PK . Modulation of DMBA-induced biochemical and histopathological changes by Syzygium cumini seed extract during skin carcinogenesis[J]. Int J Curr Biomed Pharm Res, 2011,1(2):24-30
[33] Sharma V, Sharma A, Kansal L . The effect of oral administration of Allium sativum extracts on lead nitrate induced toxicity in male mice[J]. Food Chem Toxicol, 2010,48(3):928-936
doi: 10.1016/j.fct.2010.01.002
[34] Surh YJ . Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review[J]. Food Chem Toxicol, 2002,40(8):1091-1097
doi: 10.1016/S0278-6915(02)00037-6
[1] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[2] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[3] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[4] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[5] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[6] Lin Wang, Guan-liang Wang, Jia-han Liu, Di Li, De-zeng Zhu, Liang-neng Wu . Effects of ursolic acid in ameliorating insulin resistance in liver of KKAy mice via peroxisome proliferator-activated receptors α and γ. Journal of Chinese Integrative Medicine, 2012, 10(7): 793-799.
[7] Mei-jiao Mao, Jun-ping Hu, Cong Wang, Yi-yi Zhang, Ping Liu. Effects of Chinese herbal medicine Guanxinkang on expression of PPARγ-LXRα-ABCA1 pathway in ApoE-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(7): 814-820.
[8] Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.
[9] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
[10] Yue-hua Wang, Xiao-li He, Xiao-xiu Li, Hai-lin Qin, Guan-hua Du . Effects of the effective component group of Chinese herbal medicine Xiaoxuming Decoction on brain mitochondria in rats with chronic cerebral ischemia. Journal of Chinese Integrative Medicine, 2012, 10(5): 569-576.
[11] Jing Guo, Ju-yong Wang, Zhan Zheng, Qing Wang, Chang-sheng Dong . Effects of Chinese herbal medicine Feiyanning Decoction on the ratio of CD4+CD25+ regulatory T cells and expression of transcription factor Foxp3 in mice bearing Lewis lung carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(5): 584-590.
[12] Surendra Kr. Sharma, Naveen Goyal. Protective effect of Heliotropium eichwaldi against cisplatin-induced nephrotoxicity in mice. Journal of Chinese Integrative Medicine, 2012, 10(5): 555-560.
[13] Zhan gheng, Ju-yong Wang, Qing Wang, Ling Xu, Zhen-ye Xu. Effects of Chinese herbal medicine Feiyanning decoction on expressions of nucleosome conformation-regulating factors H3-K56, Rtt109, Asf1 and E2F1 in Lewis-bearing mice. Journal of Chinese Integrative Medicine, 2012, 10(4): 448-453.
[14] Mahmoudian Alireza, Rajaei Ziba, Haghir Hossein, Banihashemian Shahaboldin, Hami Javad. Effects of valerian consumption during pregnancy on cortical volume and the levels of zinc and copper in the brain tissue of mouse fetus. Journal of Chinese Integrative Medicine, 2012, 10(4): 424-429.
[15] Gang Guo, Jian-hua Xu, Jian-hong Han, Fang Liang, Yong Zhang, Qiang Zhang, Jue Sun, Zhong-ze Fan . Chinese herbal decoction Shiquan Dabu Tang inhibits tumor growth and angiogenesis of metastasis after primary tumor surgical removal in mice. Journal of Chinese Integrative Medicine, 2012, 10(4): 436-447.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[an error occurred while processing this directive]