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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (12): 1460-1464.doi: 10.3736/jcim20121219

• Original Experimental Research • Previous Articles     Next Articles

Leaf extract of Alpinia purpurata (Vieill.) K. Schum screened for its phytochemical constituents and antibacterial and anticancer activities

Arul Raj Chinthamony1,Sophia Dominic1,Ragavendran Paramasivam1,Starlin Thangarajan2,Ahalliya Rathi Muthian3,Kanniappan Gopalakrishnan Velliyur1,2()   

  1. 1. Department of Biochemistry, Karpagam University, Coimbatore 641021, Tamilnadu, India
    2. Department of Bioinformatics, Karpagam University, Coimbatore 641021, Tamilnadu, India
    3. Department of Biochemistry, Sree Narayana Guru College, Coimbatore 641105, Tamilnadu, India
  • Received:2012-05-19 Accepted:2012-07-03 Online:2012-12-20 Published:2018-12-15

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Objective: The study was formulated with the objective to assess phytochemical constituents, antibacterial activity and anticancer activity of Alpinia purpurata.
Methods: The leaves of A. purpurata were washed thoroughly by tap water, shade dried and powdered. The plant powder was extracted with successive solvent system. Phytochemical constituents were evaluated, antibacterial activity was carried out by disc diffusion method and anticancer activity of the ethyl acetate leaf extract was evaluated by using 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results: The ethyl acetate extract of A. purpurata showed most of the phytochemicals. The extract exhibited antibacterial activity with a zone of inhibition from 5 to 14 mm at various concentrations and the extract showed potential anticancer activity against PA1 ovarian cancer cell line at the 48 h with half maximal inhibitory concentration value of 110.25 μg/mL and exhibited a dose-dependent decrease in cell count for all the concentrations tested.
Conclusion: The present study scientifically proved that ethyl acetate leaf extract of A. purpurata is a good source of phytocontituents, showing antibacterial and anticancer activities.

Key words: Alpinia, plant extracts, antibacterial agents, antineoplastic agents, in vitro

"

Extract AL SA TP FL ST CG OF TN AP CH
Petroleum ether + - + + - + - + - -
Chloroform + + + - - + - + + +
Ethyl acetate + - + + + + + + + +
Ethanol + - + + - + - + - +
Water + - + + - + - - + +

"

Tested organism Antibiotic
(Novomycin)		 Negative
control (DMSO)		 A. purpurata
(2.5 mg/mL) (5 mg/mL) (7.5 mg/mL) (10 mg/mL)
B. cereus (MTCC 441) 20 mm - 7 mm 9 mm 11 mm 12 mm
S. aureus (MTCC 96) 21 mm - 9 mm 11 mm 12 mm 14 mm
E. coli (ATCC 25922) 20 mm - 5 mm 7 mm 8 mm 10 mm
K. pneumoniae (MTCC 530) 23 mm - 9 mm 10 mm 12 mm 14 mm
S. paratyphi (MTCC 734) 23 mm - N/A N/A 6 mm 9 mm

Figure 1

Cell viability of PA1 ovarian cancer cell line incubated with A. purpurata for 48 h tested by MTT assay Data are presented as mean±standard deviation, n=3. Cells were treated with different concentrations of ethyl acetate leaf extract of A. purpurata at 48 h. Viability was quantitated by MTT assay. MTT: 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide."

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doi: 10.1002/(ISSN)1099-0844
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