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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (4): 376-380.doi: 10.3736/jcim20080410

• Original Experimental Research • Previous Articles     Next Articles

Comparative study of Scrophulariae and Aconite in inhibiting myocardial hypertrophy in rats and mice

Wei-liang Gu, Chang-xun Chen(), Ying Wang   

  1. Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2007-09-06 Online:2008-04-20 Published:2008-04-15
  • Contact: CHEN Chang-xun E-mail:cxchen6@126.com

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Objective: To explore the effects of Scrophulariae of cold nature and Aconite of hot nature on myocardial hypertrophy and neuroendocrine factors in rats and mice.
Methods: A mouse model of myocardial hypertrophy was established by hypodermic injection of isoproterenol. Sixty myocardial hypertrophy mice were randomly divided into five groups: normal control group, untreated group, metoprolol-treated group, Scrophulariae-treated group and Aconite-treated group. A rat model of myocardial hypertrophy was established by peritoneal injection of L-thyroxin. Fifty rats were randomly divided into five groups: normal control group, untreated group, captopril-treated group, Scrophulariae-treated group and Aconite-treated group. After 7-9 days of treatment with intragastric administration of the corresponding drugs, the effects of Scrophulariae and Aconite on left ventricular weight index (LVWI) and heart weight index (HWI) were determined. The concentrations of cyclic adenosine monophosphate (cAMP) in plasma and angiotensinⅡ (AngⅡ) in myocardium were detected through radio-immunity method. Cardiocyte cross-section area was determined by using HE staining.
Results: Scrophulariae of cold nature could significantly reduce the LVWI, HWI and cardiocyte cross-section area, and could decrease the content of cAMP and AngⅡ. However, Aconite had no such effects.
Conclusion: Scrophulariae of cold nature can inhibit myocardial hypertrophy through restraining the activity of sympathetic nervous system and decreasing the level of AngⅡ. The inhibition of Aconite of hot nature on cardiac hypertrophy is not significant.

Key words: Scrophulariae , Aconite, cardiomyopathy, hypertrophic, comparative study, rats, mice

CLC Number: 

  • R542.2

Table 1

Effects of Scrophulariae and Aconite on cardiac weight indexes in mice with myocardial hypertrophy induced by isoproterenol ($\bar{x}$±s, mg/g)"

Group n LVWI HWI
Normal control 12 3.02±0.23** 4.02±0.32**
Untreated 12 3.43±0.19 4.54±0.28
Metoprolol-treated 12 2.95±0.19** 3.92±0.24**
Scrophulariae-treated 12 3.00±0.27** 3.99±0.35**
Aconite-treated 12 3.34±0.16△△ 4.40±0.17△△

Table 2

Effects of Scrophulariae and Aconite on serum cAMP and myocardium AngⅡ content in mice with myocardial hypertrophy induced by isoproterenol ($\bar{x}$±s)"

Group n cAMP (nmol/L) AngⅡ (ng/L)
Normal control 10 7.8±0.5** 20.4±2.1**
Untreated 10 12.3±3.1 49.2±16.3
Metoprolol-treated 10 9.3±1.4*△ 37.1±3.6*△△
Scrophulariae-treated 10 8.9±1.1*△ 30.0±9.7**△△
Aconite-treated 10 15.7±6.0△△ 52.8±12.1△△

Table 3

Effects of Scrophulariae and Aconite on cardiac weight indexes in rats with myocardial hypertrophy induced by L-thyroxin ($\bar{x}$±s, mg/g)"

Group n LVWI HWI
Normal control 10 2.37±0.09** 3.12±0.11**
Untreated 10 3.17±0.17 4.19±0.24
Captopril-treated 10 2.87±0.16** 3.91±0.22**
Scrophulariae-treated 10 2.89±0.21** 3.85±0.31**
Aconite-treated 10 3.11±0.24△△ 4.09±0.31△△

Table 4

Effects of Scrophulariae and Aconite on myocardium AngⅡcontent in rats with myoca rdial hypertrophy induced by L-thyroxin ($\bar{x}$±s, ng/g)"

Group n AngⅡ
Normal control 10 35.0±10.6**
Untreated 10 66.3±2.8
Captopril-treated 10 50.6±9.8**△△
Scrophulariae-treated 10 59.4±6.1*△△
Aconite-treated 10 62.5±7.5△△

Table 5

Effects of Scrophulariae and Aconite on cardiocyte cross-section area in rats with myocardial hypertrophy induced by L-thyroxin ($\bar{x}$±s, pixel)"

Group n Cardiocyte cross-section area
Normal control 8 6 705±1 163**
Untreated 8 10 300±824
Captopril-treated 8 7 532±1 264**
Scrophulariae-treated 8 7 843±1 111**
Aconite-treated 8 9 679±2 029△△

Figure 1

Observation of cardiocyte cross-section area (HE staining, ×400） A: Normal control group; B: Untreated group; C: Captopril-treated group; D: Scrophulariae-treated group; E: Aconite-treated group."

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