SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (4): 381-386.doi: 10.3736/jcim20080411

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xuefu Zhuyu Capsule on endothelin-1 release in myocardium and vascular endothelium and nitric oxide/nitric oxide synthase system of swines after acute myocardial infarction and reperfusion

Xiao-lei Hou1, Bai-ling Li2, Lei Zhao1, Sheng-dong Huang2, Zhi-yun Xu2, Guan-xin Zhang2()   

  1. 1. Intensive Care Unit, Yueyang Hospital of Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
    2. Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2008-01-17 Online:2008-04-20 Published:2008-04-15
  • Contact: ZHANG Guan-xin E-mail:smmu_libailing@yahoo.com.cn

RichHTML

1

PDF (PC)

79

Like

Objective: To observe the effects of Xuefu Zhuyu Capsule (XFZYC), a compound traditional Chinese herbal medicine, on endothelin-1 (ET-1) release in myocardium and vascular endothelium and nitric oxide (NO)/nitric oxide synthase (NOS) system of swines after acute myocardial infarction (AMI) and reperfusion, and to explore the action mechanisms of XFZYC in improving the endothelium function.
Methods: Forty-five Yorkshire swines were randomized into 3 groups: sham-operated group, untreated group and XFZYC-treated group. A Yorkshire swine model of reperfusion in AMI was established by ligation of left anterior descending coronary artery for 90 min followed by 2 h relaxation. The content of serum ET-1 and NO was measured by radioimmunoassay before and after AMI and after reperfusion, respectively. Twenty-four hours after operation, all Yorkshire swines underwent diagnostic coronary angiography to delineate coronary arteries. The expressions of ET-1 and endothelial nitric oxide synthase (eNOS) in myocardial tissue of ischemic area were quantified with Western blotting. Microvessel density of the implanting sites was assessed by using HE staining.
Results: Compared with the untreated group, the levels of serum ET-1 after AMI and reperfusion were significantly decreased in XFZYC-treated group (P<0.01), while the NO levels after AMI and reperfusion in XFZYC-treated group were significantly increased (P<0.01). There was no significant difference in diagnostic coronary angiography between XFZYC-treated group and untreated group (P=0.253). Western blotting showed that the level of ET-1 in ischemic area in XFZYC-treated group was lower than that in the untreated group (P<0.01), while the eNOS protein expression in XFZYC-treated group was higher than that in the untreated group (P<0.01). The results of HE staining and microvessel density analysis of the implanting sites all showed that the degree of telangiectasis was reduced, the cardiac muscle damage was improved, and the density of capillaries was increased obviously in XFZYC-treated group as compared with the untreated group.
Conclusion: The endothelium injury may be one of the important mechanisms for no-reflow phenomenon. XFZYC may reduce the no-reflow by protecting endothelium cells.

Key words: Xuefu Zhuyu Capsule, endothelin-1, nitric oxide, nitric oxide synthase, myocardial infarction

CLC Number: 

  • R542.22

Table 1

Content of blood serum ET-1 before and after operation in three groups ($\bar{x}$±s, ng/L)"

Group n ET-1 level
Before operation 90 min after operation 60 min after reperfusion 120 min after reperfusion
Group Ⅰ 15 94.7±14.2 96.1±13.7 97.6±18.1 94.2±10.6
Group Ⅱ 13 95.5±10.8 167.7±23.5**△△ 182.7±17.8**△△ 221.6±19.7**△△
Group Ⅲ 14 93.3±11.8 124.9±19.4**△△▲▲ 141.3±15.7**△△▲▲ 156.1±21.5**△△▲▲

Table 2

Content of blood serum NO before and after operation in three groups ($\bar{x}$±s, μmol/L)"

Group n NO level
Before operation 90 min after operation 60 min after reperfusion 120 min after reperfusion
Group Ⅰ 15 57.6±21.5 55.3±14.6 58.9±23.3 58.2±11.9
Group Ⅱ 13 56.8±13.8 35.6±17.6**△△ 40.4±20.6**△△ 41.9±16.8**△△
Group Ⅲ 14 56.1±19.7 46.3±10.8*△▲▲ 52.2±18.3▲▲ 56.7±14.9▲▲

Figure 1

Coronary angiogram of the recovery of bloodstream A: Group Ⅱ (TIMI 3); B: Group Ⅲ (TIMI 2)."

Table 3

Evaluation of the recovery of bloodstream by coronary angiography"

Group n Swines in different TIMI flow grades χ2 P
TIMI 0 TIMI 1 TIMI 2 TIMI 3
Group Ⅱ 13 0 0 3 10 1.306 0.253
Group Ⅲ 14 0 0 1 13

Table 4

Expressions of ET-1 and eNOS in myocardium of swines in three groups ($\bar{x}$±s)"

Group n ET-1/β-actin eNOS/β-actin
Group Ⅰ 15 0.432±0.047 0.577±0.079
Group Ⅱ 13 0.987±0.064** 0.321±0.053**
Group Ⅲ 14 0.549±0.039△△ 0.568±0.032△△

Figure 2

Structure of cardiac muscle under optical microscope (HE staining, ×200) A: GroupⅠ; B: Group Ⅱ; C: Group Ⅲ."

Figure 3

Microvessels in cardiac muscle identified by factor Ⅷ immunohistochemical staining（Optical microscope, ×200） A: GroupⅠ; B: Group Ⅱ; C: Group Ⅲ."

[1] Reffelmann T, Kloner RA . The "no-reflow" phenomenon: basic science and clinical correlates[J]. Heart, 2002,87(2):162-168
doi: 10.1136/heart.87.2.162 pmid: 11796561
[2] Rezkalla SH, Kloner RA . No-reflow phenomenon[J]. Circulation, 2002,105(5):656-662
doi: 10.1161/hc0502.102867
[3] White FC, Carroll SM, Magnet A , et al. Coronary collateral development in swine after coronary artery occlusion[J]. Circ Res, 1992,71(6):1490-1500
doi: 10.1161/01.RES.71.6.1490 pmid: 1423941
[4] Eeckhout E, Kern MJ . The coronary no-reflow phenomenon: a review of mechanisms and therapies[J]. Eur Heart J, 2001,22(9):729-739
doi: 10.1053/euhj.2000.2172 pmid: 11350105
[5] Akiyama K, Suzuki H , Grant P, et al.Oxidation products of nitric oxide, NO2 and NO3, in plasma after experimental myocardial infarction[J]. J Mol Cell Cardiol, 1997,29(1):1-9
doi: 10.1016/S0735-1097(97)85607-0
[6] Taylor AJ, Bobik A, Richards M , et al. Myocardial endothelin-1 release and indices of inflammation during angioplasty for acute myocardial infarction and stable coronary artery disease[J]. Am Heart J, 2004,148(2):e10
doi: 10.1016/j.ahj.2004.03.064
[7] Doggrell SA .The endothelin system and its role in acute myocardial infarction[J].Expert Opin Ther Targets, 2004, 8: 191-201
doi: 10.1517/14728222.8.3.191 pmid: 15161426
[8] Bolotina VM, Najibi S, Palacino JJ , et al. Nitric oxide directly activates calcium-dependent potassium channels in vascular smooth muscle[J]. Nature, 1994,368(6474):850-853
doi: 10.1038/368850a0
[9] Wang BX, Dong XM, Guo AM , et al. Effects of Xuefu Zhuyu Decoction on functions of vascular endothelium in patients with unstable angina pectoris[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(3):256-259
王宝祥, 董雪梅, 郭爱民 , 等. 血府逐瘀汤对不稳定型心绞痛患者血管内皮功能的影响[J]. 中西医结合学报, 2006,4(3):256-259
[10] Gao CX, Ding ZS, Wo XD , et al. Experimental study of Xufu Zhuyu Tang on anti-myocardial ischemia and hypoxia tolerance[J]. Zhejiang Zhong Yi Xue Yuan Xue Bao, 2001,25(2):45-46
doi: 10.3969/j.issn.1005-5509.2001.02.033
高承贤, 丁志山, 沃兴德 , 等. 血府逐瘀汤抗心肌缺血及耐缺氧作用的实验研究[J]. 浙江中医学院学报, 2001,25(2):45-46
doi: 10.3969/j.issn.1005-5509.2001.02.033
[11] Zhou XQ, Wang DA, Luo YY , et al. Protective effect of Xuefuzhuyu(XFZHY) Decocttion on experimental ischemic myocardium[J]. Zhongguo Zhong Yi Yao Xin Xi Za Zhi, 2003,10(10):30-31
doi: 10.3969/j.issn.1005-5304.2003.10.016
周小青, 王大安, 罗尧岳 , 等. 血府逐瘀汤抗急性心肌缺血实验研究[J]. 中国中医药信息杂志, 2003,10(10):30-31
doi: 10.3969/j.issn.1005-5304.2003.10.016
[12] Xu FQ, Shi DZ, Chen KJ , et al. Effect of purified Xuefu Capsule on cardiac haemodynamics and oxygen consumption in acute myocardial ischemia of experimental dogs[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 1998,18(1):32-34
doi: 10.3321/j.issn:1003-5370.1998.01.011
徐凤芹, 史大卓, 陈可冀 , 等. 精制血府胶囊对犬急性心肌缺血心脏血流动力学和心肌耗氧量的影响[J]. 中国中西医结合杂志, 1998,18(1):32-34
doi: 10.3321/j.issn:1003-5370.1998.01.011
[13] Pernow J, Wang QD . Endothelin in myocardial ischaemia and reperfusion[J]. Cardiovasc Res, 1997,33(3):518-526
doi: 10.1016/S0008-6363(96)00265-9
[14] Watanabe T, Awane Y, Ikeda S , et al. Pharmacology of a non-selective ETA and ETB receptor antagonist, TAK-044 and the inhibition of myocardial infarct size in rats[J]. Br J Pharmacol, 1995,114(5):949-954
doi: 10.1111/bph.1995.114.issue-5
[1] Swatantra Kumar, Vimal K Maurya, Debadatta Nayak, Anil Khurana, Raj K Manchanda, Srinivasulu Gadugu, Madan LB Bhatt, Shailendra K Saxena. Calcarea carbonica treatment rescues lipopolysaccharide-induced inflammatory response in human mononuclear cells via downregulation of inducible cyclooxygenase pathway. Journal of Integrative Medicine, 2020, 18(5): 441-449.
[2] The Su Moe, Htet Htet Win, Thin Thin Hlaing, War War Lwin, Zaw Min Htet, Khin Mar Mya . Evaluation of in vitro antioxidant, antiglycation and antimicrobial potential of indigenous Myanmar medicinal plants. Journal of Integrative Medicine, 2018, 16(5): 358-366.
[3] Byung Hyuk Han, Yun Jung Lee, Jung Joo Yoon, Eun Sik Choi, Seung Namgung, Xian Jun Jin, Da Hye Jeong, Dae Gill Kang, Ho Sub Lee. Hwangryunhaedoktang exerts anti-inflammation on LPS-induced NO production by suppressing MAPK and NF-κB activation in RAW264.7 macrophages. Journal of Integrative Medicine, 2017, 15(4): 326-336.
[4] Omowumi Titilola Kayode, Musa Toyin Yakubu. Parquetina nigrescens leaves: chemical profile and influence on the physical and biochemical indices of sexual activity of male Wistar rats. Journal of Integrative Medicine, 2017, 15(1): 64-76.
[5] Rajendra Karki, Cheol-Ho Park, Dong-Wook Kim. Extract of buckwheat sprouts scavenges oxidation and inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated macrophages (RAW264.7). Journal of Chinese Integrative Medicine, 2013, 11(4): 246-252.
[6] Ju-yong Wang, Jing Guo, Chang-sheng Dong, Zhan Zheng, Qing Wang. Effects of Feiyanning Decoction, a compound traditional Chinese medicine, on iNOS and COX-2 expressions induced by tumor necrosis factor-α in lung adenocarcinoma cell line. Journal of Chinese Integrative Medicine, 2012, 10(6): 695-700.
[7] Lei Wang, Li-heng Guo, Jun Zhang, Xu-jie Zhao, Min-zhou Zhang. A study protocol for clinical pathways based on integrative medicine for patients with acute myocardial infarction. Journal of Chinese Integrative Medicine, 2011, 9(7): 725-731.
[8] Ai-ming Wu, Dong-mei Zhang, Li-xia Lou, Jian-ying Zhai, Xi-ying Lü, Li-min Chai, Shuo-ren Wang. Effects of Chinese herbal medicines Shengmai injection and Xuesaitong injection on ventricular fibrillation threshold and connexin 43 expression in rats with myocardial infarction. Journal of Chinese Integrative Medicine, 2011, 9(7): 775-782.
[9] Tapas Kumar Sur, Biswajit Auddy, Dipankar Bhattacharyya. Effects of Indian herbal formulation Body Revival on human platelet aggregation and myocardial ischemia in rats. Journal of Chinese Integrative Medicine, 2011, 9(7): 746-751.
[10] Bamidele Victor Owoyele, Omosunkanmi Toyin Adenekan, Ayodele Olufemi Soladoye. Effects of honey on inflammation and nitric oxide production in Wistar rats. Journal of Chinese Integrative Medicine, 2011, 9(4): 447-452.
[11] Zhao Li, Liang Xiao-chun, Zhang Hong, Wang Pu-yan, Sun Qing, Huang Wen-zhi, Li Bo-wu. Effects of Chinese herbal medicine Jinmaitong-medicated serum on inducible nitric oxide synthase and NADPH oxidase p22-phox subunit of rat Schwann cells cultured in high-glucose medium. Journal of Chinese Integrative Medicine, 2011, 9(3): 299-305.
[12] Gollapalle L. Viswanatha, Hanumanthappa Shylaja, K. Sadashiva Sandeep Rao, Yathiraj Ashwini, V. Ramaiah Santhosh Kumar, C. Gangadharaiah Mohan, Venkate Gowda Sunil, M. Venkateshappa Sarvesh Kumar, Subbanna Rajesh. Amelioration of immobilization stress-induced biochemical and behavioral alterations and mitochondrial dysfunction by naringin in mice: Possible mechanism of nitric oxide modulation. Journal of Chinese Integrative Medicine, 2011, 9(11): 1254-1263.
[13] Xue-ying Wang, Jie Wang, Ge Yang, Yong-mei Liu. Effects of Xuesaitong Soft Capsule on hemodynamics and cardiocyte apoptosis of rats after myocardial infarction. Journal of Chinese Integrative Medicine, 2010, 8(3): 269-274.
[14] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047.
[15] Fu-yong Chu, Jie Wang, Xiao-wei Sun , Yan-wei Xing, Kui-wu Yao, Shi-han Wang, Zhi-zhong Li. A randomized double-blinded controlled trial of Xuefu Zhuyu Capsule on short-term quality of life in unstable anginal patients with blood-stasis syndrome after percutaneous coronary intervention. Journal of Chinese Integrative Medicine, 2009, 7(8): 729-735.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3