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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (4): 381-386.doi: 10.3736/jcim20080411

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xuefu Zhuyu Capsule on endothelin-1 release in myocardium and vascular endothelium and nitric oxide/nitric oxide synthase system of swines after acute myocardial infarction and reperfusion

Xiao-lei Hou1, Bai-ling Li2, Lei Zhao1, Sheng-dong Huang2, Zhi-yun Xu2, Guan-xin Zhang2()   

  1. 1. Intensive Care Unit, Yueyang Hospital of Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
    2. Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2008-01-17 Online:2008-04-20 Published:2008-04-15
  • Contact: ZHANG Guan-xin E-mail:smmu_libailing@yahoo.com.cn

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Objective: To observe the effects of Xuefu Zhuyu Capsule (XFZYC), a compound traditional Chinese herbal medicine, on endothelin-1 (ET-1) release in myocardium and vascular endothelium and nitric oxide (NO)/nitric oxide synthase (NOS) system of swines after acute myocardial infarction (AMI) and reperfusion, and to explore the action mechanisms of XFZYC in improving the endothelium function.
Methods: Forty-five Yorkshire swines were randomized into 3 groups: sham-operated group, untreated group and XFZYC-treated group. A Yorkshire swine model of reperfusion in AMI was established by ligation of left anterior descending coronary artery for 90 min followed by 2 h relaxation. The content of serum ET-1 and NO was measured by radioimmunoassay before and after AMI and after reperfusion, respectively. Twenty-four hours after operation, all Yorkshire swines underwent diagnostic coronary angiography to delineate coronary arteries. The expressions of ET-1 and endothelial nitric oxide synthase (eNOS) in myocardial tissue of ischemic area were quantified with Western blotting. Microvessel density of the implanting sites was assessed by using HE staining.
Results: Compared with the untreated group, the levels of serum ET-1 after AMI and reperfusion were significantly decreased in XFZYC-treated group (P<0.01), while the NO levels after AMI and reperfusion in XFZYC-treated group were significantly increased (P<0.01). There was no significant difference in diagnostic coronary angiography between XFZYC-treated group and untreated group (P=0.253). Western blotting showed that the level of ET-1 in ischemic area in XFZYC-treated group was lower than that in the untreated group (P<0.01), while the eNOS protein expression in XFZYC-treated group was higher than that in the untreated group (P<0.01). The results of HE staining and microvessel density analysis of the implanting sites all showed that the degree of telangiectasis was reduced, the cardiac muscle damage was improved, and the density of capillaries was increased obviously in XFZYC-treated group as compared with the untreated group.
Conclusion: The endothelium injury may be one of the important mechanisms for no-reflow phenomenon. XFZYC may reduce the no-reflow by protecting endothelium cells.

Key words: Xuefu Zhuyu Capsule, endothelin-1, nitric oxide, nitric oxide synthase, myocardial infarction

CLC Number: 

  • R542.22

Table 1

Content of blood serum ET-1 before and after operation in three groups ($\bar{x}$±s, ng/L)"

Group n ET-1 level
Before operation 90 min after operation 60 min after reperfusion 120 min after reperfusion
Group Ⅰ 15 94.7±14.2 96.1±13.7 97.6±18.1 94.2±10.6
Group Ⅱ 13 95.5±10.8 167.7±23.5**△△ 182.7±17.8**△△ 221.6±19.7**△△
Group Ⅲ 14 93.3±11.8 124.9±19.4**△△▲▲ 141.3±15.7**△△▲▲ 156.1±21.5**△△▲▲

Table 2

Content of blood serum NO before and after operation in three groups ($\bar{x}$±s, μmol/L)"

Group n NO level
Before operation 90 min after operation 60 min after reperfusion 120 min after reperfusion
Group Ⅰ 15 57.6±21.5 55.3±14.6 58.9±23.3 58.2±11.9
Group Ⅱ 13 56.8±13.8 35.6±17.6**△△ 40.4±20.6**△△ 41.9±16.8**△△
Group Ⅲ 14 56.1±19.7 46.3±10.8*△▲▲ 52.2±18.3▲▲ 56.7±14.9▲▲

Figure 1

Coronary angiogram of the recovery of bloodstream A: Group Ⅱ (TIMI 3); B: Group Ⅲ (TIMI 2)."

Table 3

Evaluation of the recovery of bloodstream by coronary angiography"

Group n Swines in different TIMI flow grades χ2 P
TIMI 0 TIMI 1 TIMI 2 TIMI 3
Group Ⅱ 13 0 0 3 10 1.306 0.253
Group Ⅲ 14 0 0 1 13

Table 4

Expressions of ET-1 and eNOS in myocardium of swines in three groups ($\bar{x}$±s)"

Group n ET-1/β-actin eNOS/β-actin
Group Ⅰ 15 0.432±0.047 0.577±0.079
Group Ⅱ 13 0.987±0.064** 0.321±0.053**
Group Ⅲ 14 0.549±0.039△△ 0.568±0.032△△

Figure 2

Structure of cardiac muscle under optical microscope (HE staining, ×200) A: GroupⅠ; B: Group Ⅱ; C: Group Ⅲ."

Figure 3

Microvessels in cardiac muscle identified by factor Ⅷ immunohistochemical staining（Optical microscope, ×200） A: GroupⅠ; B: Group Ⅱ; C: Group Ⅲ."

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