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Journal of Integrative Medicine ›› 2021, Vol. 19 ›› Issue (1): 42-49.doi: 10.1016/j.joim.2020.09.006

• Original Experimental Research • Previous Articles     Next Articles

Anti-hemorrhoidal activity of Lian-Zhi-San, a traditional Chinese medicine, in an experimental hemorrhoidal model in rats

Hua Zhanga, Xiang-yang Yaoa, Dan-feng Zhanga, Qi-le Guoa, Jun-jun Yanga, Bao-guo Zhua, Dong-xiao Shena, Ke-feng Ruanb,c, Yuan Wangb, Jun-liang Penga   

  1. a Anorectal Department, Shanghai Jinshan TCM-Integrated Hospital, Shanghai 201501, China
    b Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    c Shanghai Zhangjiang Engineering Research Center of Modern Preparations of TCM, Shanghai 201203, China
  • Received:2020-04-14 Accepted:2020-09-07 Online:2021-01-12 Published:2021-01-22
  • Contact: Jun-liang Peng


Hemorrhoidal disease (HD) is the most common proctological disease, with an estimated prevalence rate of 4.4%, and a peak in individuals between 45 and 65 years of age. This study was done to evaluate whether Lian-Zhi-San (LZS), a clinically used anti-hemorrhoidal ointment could alleviate the inflammatory injury, with its associated changes of inflammatory cytokines and morphology of anorectal tissues, in an experimental model of HD in rats.


HD was induced by croton oil preparation (COP) applied to the anorectal region. Rats were then treated with cotton swabs soaked in LZS ointment, water or white vaseline, twice a day for 7 d. At the end of the experiment, HD was evaluated by measuring hemorrhoidal and biochemical parameters along with histopathological observations. 


In this study, COP induced a significant increase in the macroscopic severity score, anorectal coefficient and Evans blue extravasation, compared to normal rats. Additionally, it greatly enhanced the expression and secretion levels of some important inflammation-related cytokines along with marked histological damage, compared to normal rats. Rats treated with LZS ointment experienced significantly ameliorated Evans blue extravasation (P < 0.05), decreased macroscopic severity score (0.86 ± 0.14 vs. 1.65 ± 0.16) and the anorectal coefficient (P < 0.01); its use also attenuated tissue damage and inhibited the expression and secretion levels of inflammation-related cytokines (interleukin [IL]-1β, IL-6 and tumor necrosis factor-α).


This study validates a preliminary understanding of the use of LZS ointment to treat inflammatory factors and tissue damage in an experimental model of HD in rats.

Key words: Lian-Zhi-San, Hemorrhoidal disease, Anti-hemorrhoidal effect, Croton oil preparation, Berberine hydrochloride

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