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Journal of Integrative Medicine ›› 2021, Vol. 19 ›› Issue (5): 439-450.doi: 10.1016/j.joim.2021.05.002

• Original Experimental Research • Previous Articles     Next Articles

A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice

Victor Abou Nehmi Filhoa, Gilson Masahiro Muratab, Ruan Carlos Macêdo de Moraesa, Gabriely Cristina Alves Limaa, Danielle Araujo de Mirandac, Katrin Radloffd, Raquel Galvão Figuerêdo Costae, Joyce de Cassia Rosa de Jesusa, Jéssica Alves De Freitasb, Nayara Izabel Vianaf, Ruan Pimentafg, Katia Ramos Moreira Leitef, José Pinhata Otochah, Ana Flávia Marçal Pessoaai   

  1. a Laboratory of Medical Investigation (LIM-26), Department of Surgery, University of Sao Paulo Medical School, São Paulo, SP, 01246903, Brazil
    b Laboratory of Medical Investigation (LIM-29), Clinic Medical Department, University of Sao Paulo Medical School, São Paulo, SP, 01246903, Brazil
    Department of Physiology, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, 04023062, Brazil
    d Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, BW, 76131, Germany
    Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, United States
    Laboratory of Medical Investigation (LIM-55), Urology Department, University of Sao Paulo Medical School, São Paulo, SP, 01246903, Brazil
    D'Or Institute for Research and Education, Rio de Janeiro, RJ, 22281-100, Brazil
    Program in Anesthesiology, Surgical Sciences, and Perioperative Medicine, University of São Paulo, São Paulo, SP, 01246903, Brazil
    Brazilian Academic Consortium for Integrative Health (CABSIN), Natural Products Committee, São Paulo, SP, 05449-070, Brazil
  • Received:2020-10-10 Accepted:2021-03-06 Online:2021-09-15 Published:2021-09-30

Objective
To evaluate the synergic effects of a novel oral supplement formulation, containing prebiotics, yeast β-glucans, minerals and silymarin (Silybum marianum), on lipid metabolism and inflammatory and mitochondrial proteins of the liver, in control and high-fat diet-induced obese mice.

Methods
After an acclimation period, 32 male C57BL/6 mice were divided into the following groups: nonfat diet (NFD) vehicle, NFD supplemented, high-fat diet (HFD) vehicle and HFD supplemented. The vehicle and experimental formulation were administered orally by gavage once a day during the last four weeks of the diet (28 consecutive days). We then evaluated energy homeostasis, inflammation, and mitochondrial protein expression in these groups of mice.

Results
After four weeks of supplementation, study groups experienced reduced glycemia, dyslipidemia, fat, and hepatic fibrosis levels. Additionally, proliferator-activated receptor-α, AMP-activated protein kinase-1α, peroxisome proliferator-activated receptor γ co-activator 1-α, and mitochondrial transcription factor A expression levels were augmented; however, levels of IκB kinase α and p65 nuclear factor-κB expression, and oxidative markers were reduced. Notably, the cortisol/C-reactive protein ratio, a well-characterized marker of the hypothalamic-pituitary-adrenal axis immune interface status, was found to be modulated by the supplement.

Conclusion
We found that the novel supplement modified different antioxidant, metabolic and inflammatory pathways, improved energy homeostasis and the inflammatory state, and consequently alleviated hepatic steatosis.

Key words: Supplement, Silymarin, Prebiotic, Obese, Mice, Steatosis, Inflammation, Mitochondrial biogenesis

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