Search JIM Advanced Search

Journal of Integrative Medicine ›› 2015, Vol. 13 ›› Issue (1): 25-33.doi: 10.1016/S2095-4964(15)60157-1

• Research Article • Previous Articles     Next Articles

Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase

Anirudha A. Landea,Shirishkumar D. Ambavadea,Uma S. Swamia, Prafulla P. Adkara, Prashant D. Ambavadeb,Arun B. Waghamareb   

  1. a Department of Pharmacology, JSPM’s Jayawantrao Sawant College of Pharmacy and Research, University of Pune, Hadapasar, Pune-411028, India
    b Haffkine Biopharmaceutical Corporation Ltd., Pimpri, Pune-411018, India
  • Received:2014-05-24 Accepted:2014-10-30 Online:2015-01-10 Published:2015-01-15

Objective
The roots of Chlorophytum borivilanum are used in traditional medicine for the treatment of arthritis and inflammation. The aim of the work was to evaluate the anti-inflammatory activity of isolated saponins from Chlorophytum borivilianum (ISCB).
Methods
The ISCB was screened using the carrageenan-induced paw edema, histamine-induced paw edema, cotton pellet-induced granuloma, and Freund's adjuvant-induced arthritis in rats at orally administered doses of 3, 10, and 30 mg/kg. Effect of ISCB on histone deacetylase (HDAC) level was measured by the HDAC assay at the highest dose (30 mg/kg).
Results
The results showed that the ISCB significantly reduced carrageenan-induced inflammation, histamine-induced inflammation, cotton pellet-induced granuloma and Freund's adjuvant-induced arthritis in rats. The ISCB at a dose of 30 mg/kg significantly inhibited HDAC level in rat paw tissue.
Conclusion
It is concluded that saponins isolated from roots of C. borivilianum possess anti-inflammatory and anti-arthritic properties. ISCB may act by inhibiting histamine, prostaglandin and HDAC. This suggests that ISCBs have potential for therapeutic use in the treatment of inflammation and arthritis.

Key words: Chlorophytum borivilianum, Plants, Medicinal, Saponin, Carrageenan, Inflammation, Arthritis, Histon deacetylase, Rats

[1] Subhashini N, Purnima S, Devi JA, Thirupathi AT, Lavanya N . Anti-inflammatory activity of Erythrina stricta Roxb in albino rats. Int J PharmTech Res, 2011,3(2):1014-1018
[2] Wu JT, Wu LL . Acute and chronic inflammation: Effect of the risk factor(s) on the progression of the early inflammatory response to the oxidative and nitrosative stress. J Biomed Lab Sci, 2007,19(3):71-75
[3] Grivenninkov SI, Greten FR, Karin M . Immunity, inflammation, and cancer. Cell, 2010,140(6):883-889
doi: 10.1016/j.cell.2010.01.025
[4] Dhikanv V, Singh S, Pande S, Chawala A, Anand KS . Non-steroidal drug induced gastrointestinal toxicity: mechanisms and management. J Indian Acad Clin Med, 2003,4(4):315-322
[5] Elango V, Oliver C, Raghu PS . Anti-inflammatory activity of the flower extracts of Solanum nigrum in rats. Hygeia J Drugs Med, 2012,4(1):59-61
[6] Consumer Reports Health Best Buy Drugs. The nonsteroidal anti-inflammatory drugs: Treating osteoarthritis and pain. Consumers Union of United States, Inc. 2013: 1-22.
[7] Punchard NA, Whelan CJ, Adcock I . The Journal of Inflammation. J Inflamm (Lond), 2004,1(1):1-4
[8] Grausenburger R, Bilic I, Boucheron N, Zupkovitz G, El-Housseiny L, Tschismarov R, Zhang Y, Rembold M, Gaisberger M, Hartl A, Epstein MM, Matthias P, Seiser C, Ellmeier W . Conditional deletion of histone deacetylase 1 in T cells leads to enhanced airway inflammation and increased Th2 cytokine production. J Immunol, 2010,185(6):3489-3497
[9] Calixto JB, Campos MM, Santos ARS . Botanical analgesic and anti-inflammatory drugs. Ethnopharmacology, 2011,2:36-61
[10] Deore SL, Khadabadi SS . Screening of antistress properties of Chlorophytum borivilianum. Pharmacologyonline, 2009,1:320-328
[11] Rath SK, Panja AK . Clinical evaluation of root tubers of Shweta Musali(Chlorophytum borivilianum L.) and its effect on semen and testosterone. Ayu, 2013,34(3):273-275
doi: 10.4103/0974-8520.123118
[12] Panda SK, Das D, Tripathy NK . Anti-inflammatory potential of Chlorophytum borivilianum Sant. & Fern. root tubers. J Global Trends Pharm Sci, 2011,2(2):242-251
[13] Sharma SK, Kumar M . Hepatoprotective effect of Chlorophytum borivilianum root extract against arsenic intoxication. Pharmacologyonline, 2011,3:1021-1032
[14] Chakraborthy GS, Zafar R, Aeri V . Anti-inflammatory activity of methanolic extract of Chlorophytum borivilianum. J Pharm Res, 2008,1(1):58-60
[15] Panda SK, Das D, Tripathy NK . Preclinical evaluation of Chlorophytum borivilianum Sant. & Fern. root tubers. Int J Pharmacol Bio Sci, 2011,5(2):1-8
[16] Sharma G, Kumar M . Morphometroical study of seminiferous tubules of mice after using arsenic and Chlorophytum borivilianum. Pharmacologyonline, 2011,2:348-359
[17] Thakur M, Chsuhsn NS, Bhargava S, Dixit VK . A comparative study on aphrodisiac activity of some Ayurvedic herbs in male albino rats. Arch Sex Behav, 2009,38(6):1009-1015
doi: 10.1007/s10508-008-9444-8 pmid: 19139984
[18] Acharya D, Mitaine-Offer AC, Kaushik N, Miyamoto T, Paululat T, Lacaille-Dubois MA . Furostane-type steroidal saponins from the roots of Chlorophytum borivilianum. Helvetica Chimica Acta, 2008,91:2262-2269
doi: 10.1002/hlca.200890246
[19] Acharya D, Mitaine-Offer AC, Kaushik N, Miyamoto T, Pauluat T, Mirjolet JF, Duchamp O, Lacaille-Dubois MA . Cytotoxic spirostane-type saponins from the roots of Chlorophytum borivilianum. J Nat Prod, 2009,72(1):177-181
doi: 10.1021/np800559z pmid: 19128156
[20] Singh D, Pokhriyal B, Joshi YM, Kadam V . Phytopharmacological aspects of Chlorophytum borivilianum(safed musli): A review. Int J Res Phram Chem, 2012,2(3):853-859
[21] Acharya D, Mitaine-Offer AC, Kaushik N, Miyamoto T, Paululat T, Lacaille-Dubois MA . Steroidal saponins from the roots of Chlorophytum borivilianum. Planta Med. 2008; 74. PB23.
doi: 10.1055/s-0028-1084368
[22] Deore SL, Khadabadi SS . Larvicidal activity of the saponin fractions of Chlorophytum borivilianum santapau and Fernandes. J Entomol Nematol, 2009,1(5):64-66
[23] OECD Guidelines for the testing Chemicals, section 4. Test No.425. Acute oral toxicity. Statistical programme (AOT 425 Stat pgm) version: 1.0. 2010.
[24] Vogel HG . Drug discovery and evaluation: Pharmacological assays.2nd ed[M]. New York: Spinger-Verlag Berlin Heidelberg, 2002: 759-802
[25] Saénz MT, García MD, Fernández MA . Anti-inflammatory activity and acute toxicity of Anredera leptostachys. Phytomedicine, 1998,5(3):195-198
doi: 10.1016/S0944-7113(98)80027-5 pmid: 23195840
[26] ELISA kit for histone deacetylases 1 (HDAC1) organism: Rattus norvegicus (Rat) (Package insert) China: Unique product superb client favorite nicest service (USCN life science, Inc). 9th ed. 2013: 1-8.
[27] Vinegar R, Schreiber W, Hugo R . Biphasic development of carrageenan oedema in rats. J Pharmacol Exp Ther, 1969,166(1):96-103
doi: 10.1002/jps.2600581240 pmid: 5776026
[28] Lo TN, Almeida AP, Beaven MA . Dextran and carrageenan evoke different inflammatory responses in rat with respect to composition of infiltrates and effect of indomethacin. J Pharmacol Exp Ther, 1982,221(1):261-267
[29] Suleyman H, Gul HI, Gul M, Alkan M, Gocer F . Anti-inflammatory activity of bis(3-aryl-3-oxo-propyl)methylamine hydrochloride in rat. Biol Pharm Bull, 2007,30(1):63-67
doi: 10.1248/bpb.30.63
[30] Pereira LP, Silva KES, Silva RO, Assreuy AMS, Pereira MG . Anti-inflammatory polysaccharides of Azadirachta indica seed tegument. Rev Bras Farmacogn, 2012,22(3):617-622
doi: 10.1590/S0102-695X2012005000031
[31] Vasconcelos SM, Sales TM, Lima N, Lobato FG, Macedo DS, Filho MB, Leal AM, Fonteles MF, Sousa FF, Oliveira JL, Viana SB . Anti-inflammatory activities of the hydroalcoholic extracts from Erythrina velutina and E. mulungu in mice. Rev Bras Farmacogn, 2011,21(6):1155-1158
doi: 10.1590/S0102-695X2011005000134
[32] Channa S, Dar A, Anjum S, Yaqoob M, Atta-Ur-Rahman . Anti-inflammatory activity of Bacopa monniera in rodents. J Ethnopharmacol, 2006,1(2):286-289
doi: 10.1016/j.jep.2005.10.009 pmid: 16343831
[33] Rachchh MA, Yadav PN, Gokani RH, Jain SM . Anti-inflammatory activity of Benincasa hispida fruit. Int J Res Pharm Bio Sci, 2011,2(3):98-106
[34] Tamaddonfard E, Farshid AA, Hosseini L . Crocin alleviates the local paw edema induced by histamine in rats. Avicenna J Phytomed, 2012,2(2):97-104
doi: 10.4103/2231-0770.110743 pmid: 23826557
[35] Patel PP, Patil PH . Anti-inflammatory activity of saponin rich fraction isolated from the Thespesia populnea(L.) leaves. Int J Res Pharm Biomed Sci, 2012,3(4):1526-1532
[36] Katiyar NS, Rao NV, Gangwar AK . Evaluation of anti-inflammatory activity of stem extracts of Cuscuta reflexa(Roxb) in rats. Int J Res Pharm Biomed Sci, 2012,3(4):1805-1808
[37] Chitme HR, Patel NP . Antiarthritis activity of Aristolochia bracteata extract in experimental animals. Open Natural Products J, 2009,2:6-15
doi: 10.2174/1874848100902010006
[38] Rajendran R, Krishnakumar E . Anti-arthritic activity of Premna serratifolia Linn. wood against adjuvant induced arthritis. Avicenna J Med Biotech, 2010,2(2):101-106
pmid: 3558150
[39] Ekambaram S, Perumal SS, Subramanian V . Evaluation of antiarthritic activity of Strychnos potatorum Linn seeds in Freund’s adjuvant induced arthritic rat model. BMC Complement Altern Med, 2010,10(56):1-9
doi: 10.1186/1472-6882-10-1
[40] Tripathy S, Pradhan D, Anjana M . Anti-inflammatory and antiarthritic potential of Ammania baccifera Linn. Int J Res Pharm Biomed Sci, 2010,1(3):1-8
[41] Kore KJ, Shete RV, Desai NV . Anti-arthritic activity of hydroalcoholic extract of Lawsonia innermis. Int J Drug Dev Res, 2011,3(4):217-224
[42] Radhika P, Rao PR, Archana J, Rao NK . Anti-inflammatory activity of a new sphingosine derivative and cembrenoid diterpene(Lobohedleolide) isolated from marine soft corals of Sinularia crassa Tixier-Durivault and Lobophytum species of the Andaman and Nicobar Islands. Biol Pharm Bull, 2005,28(7):1311-1313
doi: 10.1248/bpb.28.1311
[43] Kaneria MS, Naik SR, Kohali RK . Anti-inflammatory, anti-arthritic and analgesic activity of a herbal formulation(DRF/AY/4012). Indian J Exp Biol, 2007,45(3):278-284
doi: 10.1097/00041433-200306000-00009 pmid: 17373374
[44] Sireeratawong S, Itharat A, Lerdvuthisopon N, Piyabhan P, Khonsung P, Boonraeng S, Jaijoy K . Anti-inflammatory, analgesic, and antipyretic activities of the ethanol extract of Piper interruptum Opiz. and Piper chaba Linn. ISRN Pharmacology, 2012,2012:480265
[45] Ramprasath VR, Shanthi P, Sachdanandam P . Anti-inflammatory effect of Semecarpus anacardium Linn. Nut extract in acute and chronic inflammatory conditions. Biol Pharm Bull, 2004,27(12):2028-2031
doi: 10.1248/bpb.27.2028
[46] Grabiec AM, Krausz S, de Jager W, Burakowski T, Groot D, Sanders ME, Prakken BJ, Maslinski W, Eldering E, Tak PP, Reedquist KA . Histone deacetylase inhibitors suppress inflammatory activation of rheumatoid arthritis patient synovial macrophages and tissue. J Immunol, 2010,184(5):2718-2728
doi: 10.4049/jimmunol.0901467
[47] Adcock IM . HDAC inhibitors as anti-inflammatory agents. Br J Pharmacol, 2007,150(7):829-831
doi: 10.1038/sj.bjp.0707166 pmid: 17325655
[1] Kayode Ezekiel Adewole. Nigerian antimalarial plants and their anticancer potential: A review. Journal of Integrative Medicine, 2020, 18(2): 92-113.
[2] Yogesh Subhash Biradar, Swathi Bodupally, Harish Padh. Evaluation of antiplasmodial properties in 15 selected traditional medicinal plants from India. Journal of Integrative Medicine, 2020, 18(1): 80-85.
[3] Dona Nirmani Ann Wijewickrama Samarakoon, Deepthi Inoka Uluwaduge, Malith Aravinda Siriwardhene. Mechanisms of action of Sri Lankan herbal medicines used in the treatment of diabetes: A review. Journal of Integrative Medicine, 2020, 18(1): 13-20.
[4] Zienab Mahmoudpour, Javad Shokri, Mohammad Kamalinejad, Neda Meftah, Soraya Khafri, Seyyed Ali Mozaffarpur, Hoda Shirafkan. The efficacy of a Persian herbal formulation on functional bloating: A double-blind randomized controlled trial. Journal of Integrative Medicine, 2019, 17(5): 344-350.
[5] Rebecca Kulbida, Alexander Mathes, Johannes Loeser. Beneficial effects of hirudotherapy in a chronic case of complex regional pain syndrome. Journal of Integrative Medicine, 2019, 17(5): 383-386.
[6] Deepak Kumar Semwal, Ashutosh Chauhan, Ankit Kumar, Sonali Aswal, Ruchi Badoni Semwal, Abhimanyu Kumar. Status of Indian medicinal plants in the International Union for Conservation of Nature and the future of Ayurvedic drugs: Shouldn’t think about Ayurvedic fundamentals?. Journal of Integrative Medicine, 2019, 17(4): 238-243.
[7] Shipra Shah, Jahangeer A. Bhat. Ethnomedicinal knowledge of indigenous communities and pharmaceutical potential of rainforest ecosystems in Fiji Islands. Journal of Integrative Medicine, 2019, 17(4): 244-249.
[8] Baxter Blonk, Ian E. Cock. Interactive antimicrobial and toxicity profiles of Pittosporum angustifolium Lodd. extracts with conventional antimicrobials. Journal of Integrative Medicine, 2019, 17(4): 261-272.
[9] Sandip B. Bharate, Vikas Kumar, Sonali S. Bharate, Bikarma Singh, Gurdarshan Singh, Amarinder Singh, Mehak Gupta, DeepikaSingh, AjayKumar, Surjeet Singh, Ram A. Vishwakarma. Discovery and preclinical development of IIIM-160, a Bergenia ciliata-based anti-inflammatory and anti-arthritic botanical drug candidate. Journal of Integrative Medicine, 2019, 17(3): 192-204.
[10] Hanae Naceiri Mrabti, Nidal Jaradat, Mohamed Reda Kachmar, Abdelaziz Ed-Dra, Abdelilah Ouahbi, Yahia Cherrah, Moulay El Abbes Faouzi. Integrative herbal treatments of diabetes in Beni Mellal region of Morocco. Journal of Integrative Medicine, 2019, 17(2): 93-99.
[11] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[12] The Su Moe, Htet Htet Win, Thin Thin Hlaing, War War Lwin, Zaw Min Htet, Khin Mar Mya . Evaluation of in vitro antioxidant, antiglycation and antimicrobial potential of indigenous Myanmar medicinal plants. Journal of Integrative Medicine, 2018, 16(5): 358-366.
[13] Bruno José Martins Da Silva, Amanda Anastácia Pinto Hage, Edilene Oliveira Silva, Ana Paula Drummond Rodrigues. Medicinal plants from the Brazilian Amazonian region and their antileishmanial activity: A review. Journal of Integrative Medicine, 2018, 16(4): 211-222.
[14] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[15] Uday P. Pratap, Hannah P. Priyanka, Karthik R. Ramanathan, Vishak Raman, Lalgi Hima, Srinivasan Thyagarajan. Noni (Morinda citrifolia L.) fruit juice delays immunosenescence in the lymphocytes in lymph nodes of old F344 rats. Journal of Integrative Medicine, 2018, 16(3): 199-207.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[3] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[4] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[5] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[6] Ming-qing Wei , Jin-zhou Tian , Jing Shi , Fu-yun Ma , Ying-chun Miao , Yong-yan Wang. Effects of Chinese medicine for promoting blood circulation and removing blood stasis in treating patients with mild to moderate vascular dementia: a randomized, double-blind and parallel-controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1240-1246
[7] Peng-fei Gao, Kenji Watanabe. Introduction of the World Health Organization project of the International Classification of Traditional Medicine. Journal of Chinese Integrative Medicine, 2011, 9(11): 1161-1164
[8] Zhang-ling Zhou, Yue-bo Jiang, Gao-bo Li, Yu-hong Gao. Analysis of facial far-infrared thermogram of patients with acute facial neuritis. Journal of Chinese Integrative Medicine, 2011, 9(11): 1221-1225
[9] Gollapalle L. Viswanatha, Hanumanthappa Shylaja, K. Sadashiva Sandeep Rao, Yathiraj Ashwini, V. Ramaiah Santhosh Kumar, C. Gangadharaiah Mohan, Venkate Gowda Sunil, M. Venkateshappa Sarvesh Kumar, Subbanna Rajesh. Amelioration of immobilization stress-induced biochemical and behavioral alterations and mitochondrial dysfunction by naringin in mice: Possible mechanism of nitric oxide modulation. Journal of Chinese Integrative Medicine, 2011, 9(11): 1254-1263
[10] Yi Chen, Wen-jian Wang. Relationships between insulin resistant and hypertension and traditional Chinese medicine intervention. Journal of Chinese Integrative Medicine, 2010, 8(12): 1114-1118