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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (1): 58-61.doi: 10.3736/jcim20040121

• Technique and Methods • Previous Articles     Next Articles

Genetic identification of internal transcribed spacers sequence in rDNA of Artemisis iwayomogi Kitam. and other two Artemisia species

Sung-yong KIM1(), Jian-wei Chen1, Zhong-quan Liu2, Yong-zhen Wang1   

  1. 1. Department of Identification of Chinese Drugs,Nanjing University of Traditional Chinese Medicine,Nanjing,Jiangsu Province 210029,China
    2. Research Institute of Genetic Resource,Nanjing Normal University,Nanjing,Jiangsu Province 210097,China
  • Online:2004-01-20 Published:2018-10-25

Objective: To make an useful identification method for the molecule of DNA on 3 herbs of Artemisia genus and compare the differences of the genes of Korean and Chinese species of Artemisia. Methods: Sequence of 3 herbs (Artemisia sacrorum Ledeb., Artemisia iwayomogi Kitam. and Artemisia capillaris Thunb.) was determined by PCR sequence system. DNA was extracted from rDNA/ITS (internal transcribed spacers) and 5.8 s. The analysis was based on the amplification through DNA sequence system. Results: There were profound differences between the Korean Artemisia and Artemisia sacrorum L.. These 2 herbs had a difference in the PCR amplifications of the agarose gel electrophoresis. There was a slight difference in the analysis of the DNA sequence system, and the substitution percentage for ITS gene fragments sequence was 3.96%.Conclusion: Analytic identification method on sequence system of ITS in rDNA is effective for these 3 herbs.

Key words: Artemisia iwayomogi Kitam., Artemisia sacrorum Ledeb., Artemisia capillalis Thunb., molecular identification, polymerase chain reaction

CLC Number: 

  • R284.1


Organization of plant nuclear ribosomal DNA internal transcribed spacer(ITS) region"


Agarose gel electrophoresis of DNA extractedfrom 3 species of ArtemisiaYin: Artemisia sacrorum Ledeb.; Bai: Artemisia iwayomogi Kitam.; Han: Artemisia capillaris Thunb.; P: extract control; M: DNA size marker: EcoRⅠ, Hind Ⅲ digested λDNA"


Agarose gel electrophoresis of PCR amplifications of gene fragment from DNA extracted from 3 species of ArtemisiaYin: Artemisia sacrorum Ledeb.; Bai: Artemisia iwayomogi Kitam.; Han: Artemisia capillaris Thunb.; P: amplify control; M:DNA size marker: EcoRⅠ, Hind Ⅲ digested λDNA"

Tab 1

Numbers of transitions/transversions (above diagonal) and substitution percentage (below diagonal) for ITS gene frag- ment sequences of 3 species of Artemisia"

Code Bai Han Yin
Bai - 14/11 27/17
Han 3.96 - 26/10
Yin 6.98 5.71 -


Sequence database of rDNA ITS gene fragment from 3 species of ArtemisiaYin: Artemisia sacrorum Ledeb.; Bai: Artemisia iwayomogi Ki-tam.; Han: Artemisia capillaris Thunb.. Dots indicate identity"

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