SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (1): 58-61.doi: 10.3736/jcim20040121

• Technique and Methods • Previous Articles     Next Articles

Genetic identification of internal transcribed spacers sequence in rDNA of Artemisis iwayomogi Kitam. and other two Artemisia species

Sung-yong KIM1(), Jian-wei Chen1, Zhong-quan Liu2, Yong-zhen Wang1   

  1. 1. Department of Identification of Chinese Drugs,Nanjing University of Traditional Chinese Medicine,Nanjing,Jiangsu Province 210029,China
    2. Research Institute of Genetic Resource,Nanjing Normal University,Nanjing,Jiangsu Province 210097,China
  • Online:2004-01-20 Published:2018-10-25

RichHTML

0

PDF (PC)

129

Cited

11

Like

Objective: To make an useful identification method for the molecule of DNA on 3 herbs of Artemisia genus and compare the differences of the genes of Korean and Chinese species of Artemisia. Methods: Sequence of 3 herbs (Artemisia sacrorum Ledeb., Artemisia iwayomogi Kitam. and Artemisia capillaris Thunb.) was determined by PCR sequence system. DNA was extracted from rDNA/ITS (internal transcribed spacers) and 5.8 s. The analysis was based on the amplification through DNA sequence system. Results: There were profound differences between the Korean Artemisia and Artemisia sacrorum L.. These 2 herbs had a difference in the PCR amplifications of the agarose gel electrophoresis. There was a slight difference in the analysis of the DNA sequence system, and the substitution percentage for ITS gene fragments sequence was 3.96%.Conclusion: Analytic identification method on sequence system of ITS in rDNA is effective for these 3 herbs.

Key words: Artemisia iwayomogi Kitam., Artemisia sacrorum Ledeb., Artemisia capillalis Thunb., molecular identification, polymerase chain reaction

CLC Number: 

  • R284.1

Fig.1

Organization of plant nuclear ribosomal DNA internal transcribed spacer（ITS） region"

Fig.2

Agarose gel electrophoresis of DNA extractedfrom 3 species of ArtemisiaYin: Artemisia sacrorum Ledeb.; Bai: Artemisia iwayomogi Kitam.; Han: Artemisia capillaris Thunb.; P: extract control; M: DNA size marker: EcoRⅠ, Hind Ⅲ digested λDNA"

Fig.3

Agarose gel electrophoresis of PCR amplifications of gene fragment from DNA extracted from 3 species of ArtemisiaYin: Artemisia sacrorum Ledeb.; Bai: Artemisia iwayomogi Kitam.; Han: Artemisia capillaris Thunb.; P: amplify control; M:DNA size marker: EcoRⅠ, Hind Ⅲ digested λDNA"

Tab 1

Numbers of transitions/transversions (above diagonal) and substitution percentage (below diagonal) for ITS gene frag- ment sequences of 3 species of Artemisia"

Code Bai Han Yin
Bai - 14/11 27/17
Han 3.96 - 26/10
Yin 6.98 5.71 -

Fig.4

Sequence database of rDNA ITS gene fragment from 3 species of ArtemisiaYin: Artemisia sacrorum Ledeb.; Bai: Artemisia iwayomogi Ki-tam.; Han: Artemisia capillaris Thunb.. Dots indicate identity"

[1] 中华人民共和国卫生部药典委员会. 中华人民共和国药典[S]. 2000年版. 北京: 化学工业出版社, 2000. 192.
[2] 凌一揆, 颜正华 .中药学(第1版)[M]. 上海: 上海科学技术出版社, 1997,( 第1版):102-103
[3] 全国韩医科大学本草学教授共同编著. 本草学(第1版)[M]. 汉城: 永林社, 1994,( 第1版):328-329
[4] 许浚 . 东医宝鉴汤液篇(卷三)(第1版)[M]. 北京: 人民卫生出版社, 1955,( 第1版):726
[5] 难波恒雄, 津田喜典 . 生药学概论(第1版)[M]. 东京: 南江堂, 1990,( 第1版):216
[6] 北村四郎, 村田, 堀腾 . 原色日本植物图鉴(草木编Ⅰ)(第1版)[M]. 东京: 保育社, 1983,( 第1版):542-543
[7] 韩大锡 . 生药学(第1版)[M]. 汉城: 东明社, 1996,( 第1版):343-344
[8] 中国科学院植物研究所. 中国高等植物图鉴(第4册)(第1版)[M]. 北京: 科学出版社, 1983,( 第1版):535
[9] 中国科学院中国植物志编辑委员会. 中国植物志(第七十六卷第二分册)[M]. 北京: 科学出版社,1991,(): 44-49
[10] WEN J, Zimmer EA . Phylogeny and biogegraphy of Panax L.(the ginseng genus,araliaceae): inferences from ITS sequences of nuclear ribosomal DNA[J]. Mol Phylogenet Evol, 1996,6(2):167-177
[11] Kohjyouma M, Nakjima S, Namera A , et al. Random amplified polymorphic DNA analysis and variation of essential oil components of Atractylodes plants[J]. Biol Pharm Bull, 1997,20(5):502-506
[12] Clark M . Plant Molecular Biology: A Laboratory Manual(1st ed)[M]. Berlin: Springer, 1997,( 1st ed):5-8
[1] De Arnab, Das Durba, Dutta Suman, Chakraborty Debrup, Boujedaini Naoual, Rahman Khuda-Bukhsh Anisur. Potentized homeopathic drug Arsenicum Album 30C inhibits intracellular reactive oxygen species generation and up-regulates expression of arsenic resistance gene in arsenite-exposed bacteria Escherichia coli. Journal of Chinese Integrative Medicine, 2012, 10(2): 210-227.
[2] Durba Das, Arnab De, Suman Dutta, Raktim Biswas, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Potentized homeopathic drug Arsenicum Album 30C positively modulates protein biomarkers and gene expressions in Saccharomyces cerevisae exposed to arsenate. Journal of Chinese Integrative Medicine, 2011, 9(7): 752-760.
[3] Thanasekaran Jayakumar, Philip Aloysius Thomas, Mathivanan Isai, Pitchairaj Geraldine. An extract of the oyster mushroom, Pleurotus ostreatus, increases catalase gene expression and reduces protein oxidation during aging in rats. Journal of Chinese Integrative Medicine, 2010, 8(8): 774-780.
[4] Wu-xuan Mei, Shao-xian Lao, Na Yu, Zheng Zhou, Lie-ping Huang, Bin Hu. Relationship between gene expressions of aquaporin 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis. Journal of Chinese Integrative Medicine, 2010, 8(2): 111-115.
[5] Ying-hong Tang, Shu-ping Zhang, Yan Liang, Chang-qing Deng. Effects of Panax notoginseng saponins on mRNA expressions of interleukin-1β, its correlative factors and cysteinyl-aspartate specific protease after cerebral ischemia-reperfusion in rats. Journal of Chinese Integrative Medicine, 2007, 5(3): 328-332.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3