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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (8): 774-780.doi: 10.3736/jcim20100808

• Original Experimental Research • Previous Articles     Next Articles

An extract of the oyster mushroom, Pleurotus ostreatus, increases catalase gene expression and reduces protein oxidation during aging in rats

Thanasekaran Jayakumara,Philip Aloysius Thomasb,Mathivanan Isaia,Pitchairaj Geraldinea   

  1. a Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
    b Institute of Ophthalmology, Joseph Eye Hospital, Tiruchirappalli 620 001, Tamil Nadu, India
  • Received:2010-04-09 Accepted:2010-05-18 Online:2010-08-20 Published:2010-08-15


The objective of the present study was to address the effect of mushroom Pleurotus ostreatus on the catalase (CAT) gene expression and the protein carbonyls in liver and kidney of aged (24 months old) rats.

Eighteen acclimated rats were divided into 3 groups of 6 each: groupⅠ, normal young (4 months old) rats; group Ⅱ, normal aged (24 months old) untreated rats; group Ⅲ, normal aged rats treated with mushroom P. ostreatus extract (200 mg/kg body weight administered intraperitoneally for 30 days). On the 31st day, rats were sacrificed by decapitation; the livers and kidneys were removed, washed free of blood, blotted dry and processed immediately. Reverse transcriptase-polymerase chain reaction (RT-PCR) and spectrophotometry were utilized for the analyses of CAT gene expression and protein carbonyl content in the tissues of livers and kidneys.

In aged rats that had been treated with the extract of P. ostreatus (group Ⅲ), the level of the transcript of CAT gene was found to be higher than that in liver (P<0.01) and kidney (P<0.05) of aged untreated (group Ⅱ) rats, respectively. Treatment of aged rats with P. ostreatus extract (group Ⅲ) resulted in protein carbonyl levels being significantly lower in liver (P<0.05) and kidney (P<0.01) than those observed in aged untreated (group Ⅱ) rats.

These results suggest that an extract of P. ostreatus can enhance the antioxidant enzyme (CAT) gene expression and could decrease the incidence of free radical-induced protein oxidation in aged rats, thereby protecting the occurrence of age-associated disorders that involve free radicals.

Key words: Pleurotus ostreatus, Catalase, Reactive oxygen species, Reverse transcriptase-polymerase chain reaction, Protein carbonyl, Rats


Figure 1

Catalase gene transcripts in the liver and kidney of different groups of rats"

Figure 2

Agarose gel electrophoresis of RT-PCR products from total RNA isolated by using TRIzol M: 100 bp DNA ladder; L1: Liver of young rats; L2: Liver of aged rats; L3: Liver of aged rats treated with mushroom extract; L4: Kidney of young rats; L2: Kidney of aged rats; L3: Kidney of aged rats treated with mushroom extract."


[1] Ashok BT, Ali R . The aging paradox: free radical theory of aging[J]. Exp Gerontol, 1999,34(3):293-303
doi: 10.1016/S0531-5565(99)00005-4
[2] Wickens AP . Ageing and the free radical theory[J]. Respir Physiol, 2001,128(3):379-391
doi: 10.1016/S0034-5687(01)00313-9 pmid: 11718765
[3] Chandra J, Samali A, Orrenius S . Triggering and modulation of apoptosis by oxidative stress[J]. Free Radic Biol Med, 2000,29(3-4):323-333
doi: 10.1016/S0891-5849(00)00302-6 pmid: 11035261
[4] Lang JD, McArdle PJ, O’Reilly PJ, Matalon S .Oxidant-antioxidant balance in acute lung injury[J]. Chest, 2002,122(6 Suppl):314S-320S
doi: 10.1378/chest.122.6_suppl.314S
[5] Suzuki T, Spitz DR, Gandhi P, Lin HY, Crawford DR . Mammalian resistance to oxidative stress: a comparative analysis[J]. Gene Expr, 2002,10(4):179-191
doi: 10.3727/000000002783992442 pmid: 12173744
[6] Park DU, Kim CH, Hong SE, Yu BP, Chung HY . Aging DB: a database for oxidative stress calorie restriction in the study of aging[J]. Age, 2003,26(1-2):11-17
doi: 10.1007/s11357-003-0002-y pmid: 23604914
[7] Yoon SO, Yun CH, Chung AS . Dose effect of oxidative stress on signal transduction in aging[J]. Mech Ageing Dev, 2002,123(12):1597-1604
doi: 10.1016/S0047-6374(02)00095-7 pmid: 12470897
[8] Kim HJ, Yu BP, Chung HY . Molecular exploration of age-related NF-kappaB/IKK downregulation by calorie restriction in rat kidney[J]. Free Radic Biol Med, 2002,32(10):991-1005
doi: 10.1016/S0891-5849(02)00798-0
[9] Kim HJ, Jung KJ, Yu BP, Cho CG, Chung HY . Influence of aging and calorie restriction on MAPKs activity in rat kidney[J]. Exp Gerontol, 2002,37(8-9):1041-1053
doi: 10.1016/S0531-5565(02)00082-7 pmid: 12213555
[10] Wiesenberg I, Missbach M, Kahlen JP, Schrder M, Carlberg C .Transcriptional activation of the nuclear receptor RZR alpha by the pineal gland hormone melatonin and identification of CGP 52608 as a synthetic ligand[J]. Nucleic Acids Res, 1995,23(3):327-333
doi: 10.1093/nar/23.3.327
[11] Fraga CG, Shigenaga MK, Park JW, Degan P, Ames BN . Oxidative damage to DNA during aging: 8-hydroxy-2′-deoxyguanosine in rat organ DNA and urine[J]. Proc Natl Acad Sci U S A, 1990,87(12):4533-4537
doi: 10.1073/pnas.87.12.4533
[12] Requena JR, Levin RL, Stadtman ER . Recent advances in the analysis of oxidized proteins[J]. Amino Acids, 2003,25(3-4):221-226
doi: 10.1007/s00726-003-0012-1
[13] Stadtman ER, Levine RL . Free radical-mediated oxidation of free amino acids and amino acid residues in proteins[J]. Amino Acids, 2003,25(3-4):207-218
doi: 10.1007/s00726-003-0011-2 pmid: 14661084
[14] Stadtman ER, Berlett BS . Reactive oxygen-mediated protein oxidation in aging and disease[J]. Chem Res Toxicol, 1997,10(5):485-494
doi: 10.1021/tx960133r
[15] Baynes JW . Role of oxidative stress in development of complications in diabetes[J]. Diabetes, 1991,40(4):405-412
[16] Sohal RS, Agarwal S, Sohal BH . Oxidative stress and aging in the Mongolian gerbil(Meriones unguiculatus)[J]. Mech Ageing Dev, 1995,81(1):15-25
doi: 10.1016/0047-6374(94)01578-A pmid: 7475349
[17] Liu J, Wang X, Shigenaga MK, Yeo HC, Mori A, Ames BN . Immobilization stress causes oxidative damage to lipid, protein, and DNA in the brain of rats[J]. FASEB J, 1996,10(13):1532-1538
doi: 10.1006/excr.1996.0334 pmid: 8940299
[18] Fang YZ, Yang S, Wu G . Free radicals, antioxidants, and nutrition[J]. Nutrition, 2002,18(10):872-879
doi: 10.1016/S0899-9007(02)00916-4 pmid: 12361782
[19] Balu M, Sangeetha P, Haripriya D, Panneerselvam C . Rejuvenation of antioxidant system in central nervous system of aged rats by grape seed extract[J]. Neurosci lett, 2005,383(3):295-300
doi: 10.1016/j.neulet.2005.04.042 pmid: 15955424
[20] Augustyniak A, Waszkiewicz E, Skrzydlewska E . Preventive action of green tea from changes in the liver antioxidant abilities of different aged rats intoxicated with ethanol[J]. Nutrition, 2005,21(9):925-932
doi: 10.1016/j.nut.2005.01.006
[21] Srinivasan M, Rukkumani R, Ram Sudheer A, Menon VP . Ferulic acid, a natural protector against carbon tetrachloride-induced toxicity[J]. Fundam Clin Pharmacol, 2005,19(4):491-496
doi: 10.1111/j.1472-8206.2005.00332.x pmid: 16011737
[22] Ng TB, Gao W, Li L, Niu SM, Zhao L, Liu J, Shi LS, Fu M, Liu F . Rose(Rosa rugosa)-flower extract increases the activities of antioxidant enzymes and their gene expression and reduces lipid peroxidation[J]. Biochem Cell Biol, 2005,83(1):78-85
doi: 10.1139/o04-100
[23] Murcia MA, Martínez-Tomé M, Jiménez AM, Vera AM, Honrubia M, Parras P . Antioxidant activity of edible fungi(truffles and mushrooms): losses during industrial processing[J]. J Food Prot, 2002,65(10):1614-1622
doi: 10.4315/0362-028X-65.10.1614
[24] Jose N, Janardhanan KK . Antioxidant and antitumour activity of Pleurotus florida[J]. Curr Sci, 2000,79(7):941-943
[25] Isai M, Elanchezhian R, Sakthivel M, Chinnakkaruppan A, Rajamohan M, Jesudasan CN, Thomas PA, Geraldine P . Anticataractogenic effect of an extract of the oyster mushroom, Pleurotus ostreatus, in an experimental animal model[J]. Curr Eye Res, 2009,34(4):264-273
doi: 10.1080/02713680902774069
[26] Jayakumar T, Thomas PA, Geraldine P . Protective effect of an extract of the oyster mushroom, Pleurotus ostreatus, on antioxidants of major organs of aged rats[J]. Exp Gerontol, 2007,42(3):183-191
doi: 10.1016/j.exger.2006.10.006
[27] Mattila P, Salo-Väänänen P, Könkö K, Aro H, Jalava T .Basic composition and amino acid contents of mushrooms cultivated in Finland[J]. J Agric Food Chem, 2002,50(22):6419-6422
doi: 10.1021/jf020608m pmid: 12381127
[28] Sambrook J, Fritsch EF, Maniatis T. Molecular cloning, a laboratory manual[M]. New York: Laboratory Cold Spring Harbor Laboratory Press,1989:
[29] Nakamura A, Goto S . Analysis of protein carbonyls with 2,4-dinitrophenyl hydrazine and its antibodies by immunoblot in two-dimensional gel electrophoresis[J]. J Biochem, 1996,119(4):768-774
doi: 10.1093/oxfordjournals.jbchem.a021306
[30] Weindruch R, Kayo T, Lee CK, Prolla TA . Gene expression profiling of aging using DNA microarrays[J]. Mech Ageing Dev, 2002,123(2-3):177-193
doi: 10.1016/S0047-6374(01)00344-X pmid: 11718811
[31] Yoon SO, Yun CH, Chung AS . Dose effect of oxidative stress on signal transduction in aging[J]. Mech Ageing Dev, 2002,123(12):1597-1604
doi: 10.1016/S0047-6374(02)00095-7 pmid: 12470897
[32] Butterfield DA, Koppal T, Howard B, Subramaniam R, Hall N, Hensley K, Yatin S, Allen K, Aksenov M, Aksenova M, Carney J .Structural and functional changes in proteins induced by free radical-mediated oxidative stress and protective action of the antioxidants N-tert-butyl-alpha-phenylnitrone and vitamin E[J].Ann N Y Acad Sci, 1998, 854: 448-462
doi: 10.1111/nyas.1998.854.issue-1
[33] Hatao H, Oh-ishi S, Itoh M, Leeuwenburgh C, Ohno H, Ookawara T, Kishi K, Yagyu H, Nakamura H, Matsuoka T .Effects of acute exercise on lung antioxidant enzymes in young and old rats[J]. Mech Ageing Dev, 2006,127(4):384-390
doi: 10.1016/j.mad.2005.12.008 pmid: 16442588
[34] Jayachandran M, Jayanthi B, Sundaravadivel B, Panneerselvam C . Status of lipid, lipid peroxidation, and antioxidant systems with Vitamin C supplementation during aging in rats[J]. J Nutr Biochem, 1996,7(5):270-275
doi: 10.1016/0955-2863(96)00027-7
[35] Valls V, Peiro C, Muñiz P, Saez GT .Age-related changes in antioxidant status and oxidative damage to lipids and dna in mitochondria of rat liver[J]. Proc Biochem, 2005,40(2):903-908
doi: 10.1016/j.procbio.2004.02.025
[36] Arivazhagan P, Thilakavathy T, Panneerselvam C . Antioxidant lipoate and tissue antioxidants in aged rats[J]. J Nutr Biochem, 2000,11(3):122-127
doi: 10.1016/S0955-2863(99)00079-0 pmid: 10742655
[37] Lombard DB, Chua KF, Mostoslavsky R, Franco S, Gostissa M, Alt FW . DNA repair, genome stability, and aging[J]. Cell, 2005,120(4):497-512
doi: 10.1016/j.cell.2005.01.028
[38] Rao G, Xia E, Richardson A . Effect of age on the expression of antioxidant enzymes in male Fisher F344 rats[J]. Mech Ageing Dev, 1990,53(1):49-60
doi: 10.1016/0047-6374(90)90033-C pmid: 2325440
[39] Limaye PV, Raghuram N, Sivakami S . Oxidative stress and gene expression of antioxidant enzymes in the renal cortex of streptozotocin-induced diabetic rats[J]. Mol Cell Biochem, 2003,243(1-2):147-152
doi: 10.1023/A:1021620414979
[40] Chen J, Rider DA, Ruan R . Identification of valid housekeeping genes and antioxidant enzyme gene expression change in the aging rat liver[J]. J Gerontol A Biol Sci Med Sci, 2006,61(1):20-27
doi: 10.1093/gerona/61.1.20
[41] Ghosh B, Hhanevold CD, Dobashi K, Orak JK, Singh I . Tissue differences in antioxidant enzyme gene expression in response to endotoxin[J]. Free Radic Biol Med, 1996,21(4):533-540
doi: 10.1203/00006450-199604001-00317 pmid: 8886805
[42] Joseph JA, Shukitt-Hale B, Casadesus G . Reversing the deleterious effects of aging on neuronal communication and behavior: beneficial properties of fruit polyphenolic compounds[J]. Am J Clin Nutr, 2005,81(1 Suppl):313S-316S
doi: 10.1093/ajcn/81.1.313S
[43] Hensley K, Hall N, Subramaniam R, Cole P, Harris M, Aksenov M, Aksenova M, Gabbita SP, Wu JF, Carney JM, Lovell M, Markesbery WR, Butterfield DA . Brain regional correspondence between Alzheimer’s disease histopathology and biomarkers of protein oxidation[J]. J Neurochem, 1995,65(5):2146-2156
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