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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 123-125.doi: 10.3736/jcim20040214

• Original Experimental Research • Previous Articles     Next Articles

An animal model of alcoholic fatty liver and the prevention and treatment with New Qinggan Decoction

Guo-jie Hu1, Dian-gui Li2, Shi-fang Li1, Zhen-bin Li3, Jun-xia Li3   

  1. 1. Department of Infectious Diseases, General Hospital of Huabei Oil Field,Renqiu,Hebei Province 062552, China
    2. Hebei Staff Medical College, Baoding, Hebei Province 071000, China
    3. Department of Traditional Chinese Medicine, Bethune International Peace Hospital, Shijiazhuang, Hebei Province 050073, China
  • Online:2004-03-20 Published:2018-10-26

Objective: To establish an animal model of alcoholic fatty liver (AFL) and to observe the prevention and treatment with New Qinggan Decoction (NQGD). Methods: Female SD rats were exposed to the mixture of white spirit and corn oil continuously for up to 6 weeks via an intragastric feeding protocol. The pathologic alteration of the liver and the changes of serum lipid were observed, and the effect of NQGD for preventing and treating AFL was studied. Results: Wide hepatic steatosis was induced in model group rats. Serum triglyceride (TG) and total cholesterol (TC) of the model group were higher than those of the normal group (P<0.01). Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of the model group were higher than those of the normal group (P<0.05). In NQGD group, hepatic steatosis was milder than that in the model group, and serum TG, TC, AST, ALT descended significantly as compared with the model group (P<0.01). Conclusion: AFL model of rats established by feeding the mixture of white spirit and corn oil is similar to the episode of the human AFL, and is easy to intervene with medicine. NQGD can prevent the occurrence of AFL in this model.

Key words: alcoholic fatty liver, animal model, rat, New Qinggan Decoction

CLC Number: 

  • R575.5

Figure 1

Histomorphological examination of SD rat liver tissue (HE staining,×200)A: Normal group; B: Model group; C: NQGD low dose group; D: NQGD high dose group"

Tab 1

Serum TG and TC of rats in 4 groups (ヌ±S, mmol/L)"

Group n TG TC
Normal 8 0.77±0.12 1.84±0.14
Model 8 1.19±0.30** 2.21±0.31**
NQGD high dose 8 0.74±0.17△△ 1.67±0.19△△
NQGD low dose 8 0.79±0.27△△ 1.68±0.22△△

Tab 2

Serum AST and ALT of rats in 4 groups (ヌ±S ,mmol/L)"

Group n AST ALT
Normal 8 204.85±46.23 36.92±4.43
Model 8 241.67±23.88* 43.71±4.42*
NQGD high dose 8 171.09±24.44△△ 32.61±4.84△△
NQGD low dose 8 193.98±32.90△△ 36.20±4.38△△
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