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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (3): 216-218.doi: 10.3736/jcim20040320

• Technique and Methods • Previous Articles     Next Articles

A preliminary report on solid-state fermentation of Ganoderma lucidum with Radix Astragali containing medium

Hua-zhen Chen, Jian-wei Chen   

  1. Institute of Medicinal Fungi & Bio-Technology of Traditional Chinese Medicine, College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
  • Online:2004-06-20 Published:2018-10-25

Objective: To test the practicability of the solid-state fermentation for medicinal fungi by fermenting Ganoderma lucidum with Radix Astragali containing medium. Methods: Ganoderma lucidum was fermented in ordinary medium, drug-containing medium (containing Radix Astragali) and selenium-rich drug-containing medium respectively. The polysaccharide contents of fermentation products from the three kinds of culture media were tested at different time, and the changes were compared. Results: The polysaccharide contents of fermentation products from the three kinds of culture media were 4.65%, 3.76% and 4.50% respectively and their relative standard deviation were 1.61%, 1.99% and 1.86% respectively. By observing the changes of the contents of polysaccharide, protein and total saponin in fermentation products from the drug-containing medium at different time, it was found that the 28th fermentation day was the time when secondary metabolism was most active, and it should be the fermented terminal point. Conclusion: The fermentative combination of Ganoderma lucidum and Radix Astragali is practicable.

Key words: fermentation, Ganoderma lucidum, Radix Astragali,  polysaccharide

CLC Number: 

  • R283.1

Tab 1

Dry rates and polysaccharide contents of fermentation products from three culture media"

Culture medium Dry Wet weight Dry weight Dry rate Consumption Extracting rate Contents of RSD of
weight of of of (%) rate(%) of unrefined polysaccharide contents of
matrix fermentation fermentation polysaccharide (%) polysaccharide
(g) products products (%) (%)
(g) (g)
Ordinary medium 150 283 105 37.1 30.1 7.6 4.65 1.61
Drug-containing medium 150 279 103 37.1 31.3 8.01 3.76 1.99
Selenium-rich 150 278 105 37.6 30.5 9 4.5 1.86
drug-containing medium


Tested substance Contents at different fermentation time
10 d 15 d 20 d 25 d 28 d 30 d 40 d 50 d
Polysaccharide 3.11 2.74 2.30 1.95 1.21 1.51 1.33 1.50
Protein 7.01 11.58 11.43 13.83 6.91 10.39 13.36 9.82
Total saponin 1.98 3.26 2.70 1.91 1.37 1.60 1.28 1.19
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