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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (2): 170-173.doi: 10.3736/jcim20070214

• Original Experimental Research • Previous Articles     Next Articles

Effects of Huoxue Bushen Mixture on skin blood vessel neogenesis and vascular endothelial growth factor expression in hair follicle of C57BL/6 mice

Shang-pu Gao1, Lan Huang2, Xin-wei Yang3   

  1. 1. Department of Dermatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
    3. Department of Traditional Chinese Surgery, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai 200071, China
  • Online:2007-03-31 Published:2007-03-15

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Objective: To investigate the possible stimulating mechanism of Huoxue Bushen Mixture (HXBSM), a traditional Chinese compound medicine, on hair growth of mice via measuring the variance of skin blood vessel neogenesis and vascular endothelial growth factor (VEGF) expression in the hair follicle. 

Methods: Hot rosin and paraffin mixture depilation were used to induce C57BL/6 mice hair follicle to enter from telogen into anagen. Ninety C57BL/6 mice were divided into 3 groups randomly: HXBSM group, Yang-xue Shengfa Capsule (YXSFC, another traditional Chinese compound medicine) group and untreated group. The mice were fed with corresponding drugs after modeling. The hair growth of the mice was observed every day. Every ten mice out of each group were executed respectively at day 4, 11 and day 17. Skin blood vessel neogenesis was counted through pathological section and VEGF expression in the hair follicle was measured via immunohistochemical method. 

Results: The number of local blood vessel neogenisis in the HXBSM group observed was larger than that in the untreated group at day 4 (P＜0.05) ; and evidently larger than that in the YXSFC group and the untreated group at day 11 (P＜0.05). The expression of VEGF in the hair follicle was distinctively higher than that in the YXSFC group and the untreated group at day 11 and day 17 (P＜0.05). 

Conclusion: HXBSM up-regulates VEGF expression to accelerate blood vessel neogenesis and hair growth.

Key words: traditional Chinese medicine, blood vessel, vascular endothelial growth factor, immunohistochemistry, hair follicle, mice

CLC Number: 

  • R285.5

Table 1

Skin blood vessel neogenesis and expression of VEGF in hair follicle of mice in three groups ($\bar{x}±S$）"

GroupnNumber of skin blood vesselExpression of VEGF (%)
HXBSM
Day 41012±3▲ 21.67±2.34
Day 111016±3*▲□ 29.00±2.00*▲□
Day 17915±3*37.17±2.86*△▲□
YXSFC
Day 41011±220.17±1.17
Day 111012±3▲ 23.83±1.60*
Day 17912±227.33±3.39*△▲
Untreated
Day 4109±121.33±2.07
Day 11108±223.67±1.03*
Day 17811±2*△ 24.67±2.16*

Figure 1

The eleventh day's VEGF expression of mice hair follicle in three groupsA: HXBSM group; B: YXSFC group; C: Untreated group."

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doi: 10.1080/00015550260132622 pmid: 12223959
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