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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (9): 934-938.doi: 10.3736/jcim20080911

• Original Experimental Research • Previous Articles     Next Articles

Bushen Rougan Recipe in prevention of hepatic fibrosis in rats induced by dimethylnitrosamine: a study on its preliminary mechanism

Bin Zhang1(), Ling-tai Wang2   

  1. 1. Department of Oncology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
    2. Department of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
  • Received:2008-05-18 Online:2008-09-20 Published:2008-09-15

Objective: To study the effects of Bushen Rougan Recipe (BSRGR), a compound traditional Chinese herbal medicine, on hepatic fibrosis in rats induced by dimethylnitrosamine (DMN), and to explore its preliminary mechanism.
Methods: A total of 40 male Wistar rats were randomly divided to normal control group (n=10), untreated group (n=15), and BSRGR group (n=15). Except for the rats in normal control group, hepatic fibrosis in rats was induced by peritoneal injection of DMN for 4 weeks. And the rats in the BSRGR group were also intragastrically administered BSRGR within the 4-week course. At the end of the 4-week course, rats were all sacrificed. The liver functions were determined by automatic biochemistry analyzer, including serum total bilirubin (Tbil), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin. Expressions of connective tissue growth factor (CTGF) and collagen type Ⅰ mRNA in liver tissue were detected by reverse transcription polymerase chain reaction.
Results: It was found that the serum Tbil level and the activities of AST, ALT were declined in the BSRGR group as compared with those in the untreated group (P<0.01). The serum albumin content in the BSRGR group was increased as compared with that in the untreated group (P<0.01). The expressions of collagen type Ⅰ and CTGF mRNAs in the untreated group were higher than those in the BSRGR group (P<0.01).
Conclusion: BSRGR can decrease the expressions of collagen type Ⅰ and CTGF mRNAs in the rats with hepatic fibrosis, which may be one of possible mechanisms in treating hepatic fibrosis.

Key words: Bushen Rougan Recipe, hepatic fibrosis, dimethylnitrosamine, collagen type I, connective tissue growth factor, rats

CLC Number: 

  • R285.5

Table 1

Serum ALT, AST activities and albumin, Tbil levels in different groups(x±s)"

Group n Tbil (μmol/L) ALT (U/L) AST (U/L) Albumin (g/L)
Normal control 10 1.49±0.15 34.10±5.65 125.70±13.91 38.75±5.08
Untreated 13 35.46±4.59** 94.46±10.71** 284.38±38.86** 25.42±2.52**
BSRGR 14 22.14±5.71△△ 74.92±7.71△△ 224.01±13.19△△ 28.64±3.78△△

Figure 1

Expression of CTGF mRNA in liver tissue of rats in different groups Lane 1-3: Amplified products of β-actin (300 bp); Lane 4-6: Amplified products of CTGF mRNA in normal control group, untreated group and BSRGR group, respectively (383 bp)."

Figure 2

Expression of collagen type Ⅰ mRNA in liver tissue of rats in different groups Lane 1-3: Amplified products of β-actin (300 bp); Lane 4-6: Amplified products of collagen type Ⅰ mRNA in normal control group, untreated group and BSRGR group, respectively (225 bp)."

[1] Attallah AM, Mosa TE, Omran MM , et al. Immunodetection of collagen types Ⅰ, Ⅱ, Ⅲ, and Ⅳ for differentiation of liver fibrosis stages in patients with chronic HCV[J]. J Immunoassay Immunochem, 2007,28(2):155-168
doi: 10.1080/15321810701212088
[2] Zhang C, Zhu Y, Wan J , et al. Effects of Ginkgo biloba extract on cell proliferation, cytokines and extracellular matrix of hepatic stellate cells[J]. Liver Int, 2006,26(10):1283-1290
doi: 10.1111/liv.2006.26.issue-10
[3] Rachfal AW, Brigstock DR . Connective tissue growth factor(CTGF/CCN2) in hepatic fibrosis[J]. Hepatol Res, 2003,26(1):1-9
doi: 10.1016/S1386-6346(03)00115-3 pmid: 12787797
[4] Gressner AM, Yagmur E, Lahme B , et al. Connective tissue growth factor in serum as a new candidate test for assessment of hepatic fibrosis[J]. Clin Chem, 2006,52(9):1815-1817
doi: 10.1373/clinchem.2006.070466
[5] Sakaida I, Hironaka K, Terai S , et al. Gadolinium chloride reverses dimethylnitrosamine(DMN)-induced rat liver fibrosis with increased matrix metallo-proteinases(MMPs) of Kupffer cells[J]. Life Sci, 2003,72(8):943-959
doi: 10.1016/S0024-3205(02)02342-1
[6] Zhang B, Wan MB, Wang LT . Expression of Ⅳ-C and TGF-β1 protein in rat with hepatic fibrosis induced by DMN and the therapeutic effects of Chinese traditional medicine[J]. Zhong Yi Yao Xue Bao, 2005,33(4):25-27
doi: 10.3969/j.issn.1002-2392.2005.04.014
张斌, 万谟彬, 王灵台 . 中药复方对DMN诱导的肝纤维化大鼠Ⅳ型胶原和TGF-β1蛋白表达的影响[J]. 中医药学报, 2005,33(4):25-27
doi: 10.3969/j.issn.1002-2392.2005.04.014
[7] Zhang B, Wan MB, Wang LT . Therapeutic effect of Bushen Rougan Recipe on hepatic fibrosis in rats[J]. Zhong Xi Yi Jie He Xue Bao, 2005,3(2):132-135
doi: 10.3736/jcim20050214
张斌, 万谟彬, 王灵台 . 补肾柔肝方治疗大鼠肝纤维化的实验研究[J]. 中西医结合学报, 2005,3(2):132-135
doi: 10.3736/jcim20050214
[8] Parsons CJ, Takashima M, Rippe RA . Molecular mechanisms of hepatic fibrogenesis[J]. J Gastroenterol Hepatol, 2007,22(Suppl 1):S79-S84
doi: 10.1111/j.1440-1746.2006.04659.x pmid: 17567474
[9] Kisseleva T, Brenner DA . Role of hepatic stellate cells in fibrogenesis and the reversal of fibrosis[J]. J Gastroenterol Hepatol, 2007,22(Suppl 1):S73-S78
doi: 10.1111/j.1440-1746.2006.04658.x pmid: 17567473
[10] Brocks B, Kraft S, Zahn S , et al. Generation and optimization of human antagonistic antibodies against TIMP-1 as potential therapeutic agents in fibrotic diseases[J]. Hum Antibodies, 2006,15(4):115-124
[11] Sentíes-Góámez MD, Gélvez-Gastílum FJ, Meza-García E , et al. Hepatic fibrosis: role of matrix metallo-proteases and TGFbeta[J]. Gac Med Mex, 2005,141(1):315-322
pmid: 16164129
[12] Campbell JS, Hughes SD, Gilbertson DG , et al. Platelet-derived growth factor C induces liver fibrosis, steatosis, and hepatocellular carcinoma[J]. Proc Natl Acad Sci U S A, 2005,102(9):3389-3394
doi: 10.1073/pnas.0409722102
[13] Li G, Xie Q, Shi Y , et al. Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats[J]. J Gene Med, 2006,8(7):889-900
doi: 10.1002/jgm.894 pmid: 16652398
[14] George J , Tsutsumi M.siRNA-mediated knockdown of connective tissue growth factor prevents N-nitrosodimethylamine-induced hepatic fibrosis in rats[J]. Gene Ther, 2007,14(10):790-803
doi: 10.1038/
[15] Weng HL, Ciuclan L, Liu Y , et al. Profibrogenic transforming growth factor-beta/activin receptor-like kinase 5 signaling via connective tissue growth factor expression in hepatocytes[J]. Hepatology, 2007,46(4):1257-1270
doi: 10.1002/hep.v46:4
[16] Hora C, Negro F, Leandro G , et al. Connective tissue growth factor, steatosis and fibrosis in patients with chronic hepatitis C[J]. Liver Int, 2008,28(3):370-376
doi: 10.1111/j.1478-3231.2007.01608.x pmid: 17976159
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