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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (12): 1286-1289.doi: 10.3736/jcim20081215

• Original Experimental Research • Previous Articles     Next Articles

Extraction of praeruptorin A from Radix Peucedani by supercritical fluid extaction-CO2

Ai-dong Liu1, Wei Hou2, Xue-song Chen3, Zhi-dong Qiu4()   

  1. 1. Department of Cardiology, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, Jilin Province 130021, China
    2. Institute of Special Wild Economic Animal and Plant, Chinese Academy of Agricultural Sciences, Changchun, Jilin Province 132109, China
    3. Department of Research and Development, Chuangzhi Medical Development Company Limited, Jilin Province, Changchun, Jilin Province 130051, China
    4. Department of Pharmaceutics, College of Pharmacy, Changchun University of Traditional Chinese Medicine, Changchun, Jilin Province 130117, China
  • Received:2008-04-20 Online:2008-12-20 Published:2008-12-15

Objective:

To extract praeruptorin A from Radix Peucedani by supercritical fluid extraction (SFE)-CO2.


Methods: 

After preliminary experiment, three main factors were acquired that could influence the result of SFE-CO2, including the time, pressure and temperature of the extraction. The optimal extraction process was carried out on orthogonal design, and SFE-CO2 was compared with the traditional methods.


Results: 

In the extraction of the praeruptorin A, the best extraction conditions were 60 ℃, 20 MPa, and duration for three hours. As cosolvent alcohol was added, the amount of extraction of the praeruptorin A increased, and the amount of SFE-CO2 extraction was higher than those of decoction and heating reflux.


Conclusion:

The SFE-CO2 in extracting praeruptorin A from Radix Peucedani is feasible and reliable.

Key words: chromatography, supercritical fluid, Peucedanum decursivum, praeruptorin A

CLC Number: 

  • R284.2

Table 1

Factors and levels in SFE-CO2 method"

Level Factor
A: Extract temperature (℃) B: Extract pressure (MPa) C: Extract time (h)
1 40 20 1
2 50 30 2
3 60 40 3

Table 2

Result of orthogonal experiment"

No. A B C D (blank) Extraction of praeruptorin A (mg)
1 1 1 1 1 7.78
2 1 2 2 2 13.08
3 1 3 3 3 16.27
4 2 1 2 3 15.63
5 2 2 3 1 19.45
6 2 3 1 2 13.30
7 3 1 3 2 22.27
8 3 2 1 1 15.66
9 3 3 2 3 17.31
j 37.13 45.68 36.74 44.54
j 48.38 48.19 46.02 48.65
j 55.24 46.88 57.99 47.56

Table 3

Result of analysis of variance"

Source SS Degree of freedom Mean square F P
A 6.036 665 2 55.732 7 53.036 47 <0.05
B 0.836 667 2 1.050 8 2.876 22 >0.05
C 7.083 333 2 75.662 6 72.002 18 <0.05
D 1.370 000 2 3.022 4 1 >0.05
[1] Zhang YJ, Yang PQ . Progress on the extraction of chemical constituents in traditional Chinese materia medica by supercritical CO2 extraction[J]. Hua Xi Yao Xue Za Zhi, 1998,13(4):242-244, 246
张印俊, 杨培全 . 超临界二氧化碳流体萃取技术提取中草药成分的进展[J]. 华西药学杂志, 1998,13(4):242-244, 246
[2] Yang T, Li JH . Research progress in supercritical fluid extraction of pharmaceuticals[J]. Hua Gong Ye Jin, 1997,18(4):377-384
杨涛, 李静海 . 超临界流体萃取天然药物的研究现状及发展趋势[J]. 化工冶金, 1997,18(4):377-384
[3] Ge FH, Li J, Wang HB , et al. The latest advancement and prospect of supercritical fluid extraction of CO2 in natural product extraction and pharmaceutical analysis[J]. Zhong Yao Cai, 1995,18(6):316-319
葛发欢, 李菁, 王海波 , 等. 超临界CO2流体萃取技术在天然产物提取及药物分析中的最新研究进展和前景[J]. 中药材, 1995,18(6):316-319
[4] Yuan YF, Zhou J, Zheng XM , et al. Study on chemical composition of Rhixoma Chuanxiong aetherolea extracted by supercritical fluid extraction of CO2[J]. Zhongguo Yao Xue Za Zhi, 2000,35(2):84-87
doi: 10.3321/j.issn:1001-2494.2000.02.005
原永芳, 周践, 郑晓梅 , 等. 超临界流体CO2萃取川芎挥发油化学成分的研究[J]. 中国药学杂志, 2000,35(2):84-87
doi: 10.3321/j.issn:1001-2494.2000.02.005
[5] Liu Y, Wang LN, Du XF . The application of supercritical extraction technology in Chinese medicinal herbs[J]. Liaoning Zhong Yi Xue Yuan Xue Bao, 2005,7(3):283-284
doi: 10.3969/j.issn.1673-842X.2005.03.076
刘洋, 王丽娜, 杜晓峰 . 超临界萃取技术在中药方面的应用[J]. 辽宁中医学院学报, 2005,7(3):283-284
doi: 10.3969/j.issn.1673-842X.2005.03.076
[6] Wang CZ, Wu ZH, Hu XM , et al. The application of supercritical extraction technology in research and development of new Chinese medicinal drugs[J]. Zhong Yao Yan Jiu Yu Xin Xi, 2005,7(2):20-23, 44
doi: 10.3969/j.issn.1673-4890.2005.02.008
王承智, 吴志宏, 胡筱敏 , 等. 超临界流体萃取技术在中药新药研发中的应用[J]. 中药研究与信息, 2005,7(2):20-23, 44
doi: 10.3969/j.issn.1673-4890.2005.02.008
[7] Yan JZ, Tong SQ, Jin J , et al. Application of supercritical extraction technology in analysis of effective components in traditional Chinese medicine[J]. Shi Zhen Guo Yi Guo Yao, 2004,15(3):179-180
doi: 10.3969/j.issn.1008-0805.2004.03.043
颜继忠, 童胜强, 金洁 , 等. 超临界流体技术在中草药有效成分分析中的应用[J]. 时珍国医国药, 2004,15(3):179-180
doi: 10.3969/j.issn.1008-0805.2004.03.043
[8] Zhang C, Li L, Geng LD , et al. Pharmacognostic studies on Desmodium gyrans[J]. Zhongguo Zhong Yao Za Zhi, 2005,30(3):177-179
doi: 10.3321/j.issn:1001-5302.2005.03.004
张村, 李丽, 耿立冬 , 等. 白花前胡质量标准研究[J]. 中国中药杂志, 2005,30(3):177-179
doi: 10.3321/j.issn:1001-5302.2005.03.004
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