小续命汤有效成分组的高通量筛选研究

doi:10.3736/jcim20060117

Abstract

Objective

To present a new approach for the research of effective components extracted from compounds of traditional Chinese medicine and their action mechanisms by high-throughput screening assay.

Methods

We observed the anti-oxidation activities of 240 sequential components (L1-L120 and A1-A120) extracted from Xiaoxuming Recipe, the effects of these components on SH-SY5Y cells with H2O2-induced and L-glutamic acid-induced damages and the levels of resting calcium ion in neurocytes of rats.

Results

Some components (L1-L40, A100-A120) extracted from Xiaoxuming Recipe had the corresponding effects listed above. The combination of these components was regarded as the groups of effective components of Xiaoxuming Recipe in treating sequelae resulting from brain ischemia.

Conclusion

Xiaoxuming Recipe has protective effect on brain-ischemia-induced damages through the actions of multiple components with multiple targets.

Key words: High-throughput screening, Xiaoxuming Decoction, Brain ischemia

CLC Number:

R285

Yue-hua Wang, Hai-xia Zhang, Qi Li, Yi Ding, Juan-juan Hu, Guan-hua Du. High-throughput screening assay for groups of effective components extracted from Xiaoxuming Recipe. Journal of Chinese Integrative Medicine, 2006, 4(1): 64-67.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060117

http://www.jcimjournal.com/EN/Y2006/V4/I1/64

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High-throughput screening assay for groups of effective components extracted from Xiaoxuming Recipe

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